Cobalt in PDB 8sfk: Crystal Structure of the Engineered Ssopox Variant IVE2

All present enzymatic activity of Crystal Structure of the Engineered Ssopox Variant IVE2:
3.1.8.1;

The structure of Crystal Structure of the Engineered Ssopox Variant IVE2, PDB code: 8sfk was solved by P.Jacquet, R.Billot, A.Shimon, N.Hoekstra, C.Bergonzi, A.Jenks, D.Daude, M.H.Elias, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	49.48 / 1.40
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	66.06, 74.67, 137.4, 90, 90, 90
R / Rfree (%)	13.5 / 16.9

The structure of Crystal Structure of the Engineered Ssopox Variant IVE2 also contains other interesting chemical elements:

Iron

(Fe)

1 atom

The binding sites of Cobalt atom in the Crystal Structure of the Engineered Ssopox Variant IVE2 (pdb code 8sfk). This binding sites where shown within 5.0 Angstroms radius around Cobalt atom.
In total only one binding site of Cobalt was determined in the Crystal Structure of the Engineered Ssopox Variant IVE2, PDB code: 8sfk:

Cobalt binding site 1 out of 1 in the Crystal Structure of the Engineered Ssopox Variant IVE2


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 1 of Crystal Structure of the Engineered Ssopox Variant IVE2 within 5.0Å range: probe atom residue distance (Å) B Occ D:Co402 b:13.2 occ:1.00 O D:HOH567 1.9 14.5 1.0 OQ2 D:KCX137 2.1 11.8 1.0 ND1 D:HIS170 2.1 13.4 1.0 NE2 D:HIS199 2.1 12.3 1.0 CE1 D:HIS199 3.0 12.1 1.0 CE1 D:HIS170 3.0 12.7 1.0 CX D:KCX137 3.0 11.2 1.0 CG D:HIS170 3.1 11.9 1.0 CD2 D:HIS199 3.2 12.2 1.0 OQ1 D:KCX137 3.3 12.1 1.0 FE D:FE401 3.4 11.7 1.0 CB D:HIS170 3.5 11.8 1.0 NH2 D:ARG223 3.7 15.5 1.0 OD2 D:ASP256 4.1 14.1 1.0 NE2 D:HIS170 4.2 13.1 1.0 ND1 D:HIS199 4.2 12.7 1.0 CE1 D:HIS22 4.2 11.8 1.0 CD2 D:HIS170 4.2 13.6 1.0 NZ D:KCX137 4.2 11.7 1.0 NE2 D:HIS22 4.2 12.1 1.0 CA D:HIS170 4.2 11.4 1.0 CG D:HIS199 4.3 12.7 1.0 CZ D:ARG223 4.5 14.6 1.0 CD1 D:LEU97 4.6 20.1 1.0 CE D:KCX137 4.7 11.7 1.0 OD1 D:ASP256 4.8 12.7 1.0 NE D:ARG223 4.8 14.8 1.0 CG D:ASP256 4.8 12.3 1.0

P.Jacquet, R.Billot, A.Shimon, N.Hoekstra, C.Bergonzi, A.Jenks, E.Chabriere, D.Daude, M.H.Elias. Changes in Active Site Loops Conformation Relates to A Transition From Lactonase to Phosphotriesterase To Be Published.