Cobalt in the structure of E. Coli Methionine Aminopeptidase: Trifluoromethionine Complex (pdb 1c22)

The binding sites of Cobalt atom in the structure of E. Coli Methionine Aminopeptidase: Trifluoromethionine Complex (pdb code 1c22). This binding sites where shown with 5.0 Angstroms radius around Cobalt atom. The 1c22 structure was solved by W.T.LOWTHER, Y.ZHANG, P.B.SAMPSON, J.F.HONEK, B.W.MATTHEWS, with X-Ray Crystallography technique, brief refinement statistics is given in the table below: Resolution (A) 27.1-1.8 Space group P1211 a (A) 39.128 b (A) 67.604 c (A) 48.826 alpha (°) 90.00 beta (°) 111.05 gamma (°) 90.00 Rfactor (%) n/a Rfree (%) n/a Cobalt Binding Sites: Cobalt binding site 1 out of 2 in 1c22 Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 1 of Cobalt in the PDB 1c22. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Cobalt atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: A: Asp108, A: His171, A: His178, A: Thr202, A: Glu204, A: Glu235, A: Co402, A: Mf3300, A: Hoh603, conact list: Atom Atom Distance (A) Co CB A:Asp108 4.20 Co OD2 A:Asp108 3.50 Co OD1 A:Asp108 1.97 Co CG A:Asp108 2.95 Co NE2 A:His171 2.14 Co ND1 A:His171 4.28 Co CD2 A:His171 3.09 Co CE1 A:His171 3.15 Co CG A:His171 4.27 Co NE2 A:His178 4.83 Co CB A:Thr202 4.11 Co CG2 A:Thr202 3.89 Co OG1 A:Thr202 3.59 Co OE1 A:Glu204 3.32 Co CB A:Glu204 4.92 Co OE2 A:Glu204 2.06 Co CD A:Glu204 3.04 Co CG A:Glu204 4.33 Co OE1 A:Glu235 2.02 Co OE2 A:Glu235 3.37 Co CD A:Glu235 2.99 Co CG A:Glu235 4.35 Co CO A:Co402 3.38 Co O A:Mf3300 1.99 Co N A:Mf3300 4.46 Co C A:Mf3300 2.82 Co CA A:Mf3300 4.15 Co OXT A:Mf3300 3.16 Co O A:Hoh603 4.72 interactive model: Cobalt binding site 2 out of 2 in 1c22 Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 2 of Cobalt in the PDB 1c22. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Cobalt atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: A: Asp97, A: Val98, A: Thr99, A: Asp108, A: Thr109, A: Glu204, A: Glu235, A: Co401, A: Mf3300, A: Hoh504, A: Hoh557, A: Hoh570, A: Hoh603, conact list: Atom Atom Distance (A) Co CB A:Asp97 4.03 Co OD2 A:Asp97 2.33 Co C A:Asp97 4.95 Co OD1 A:Asp97 2.12 Co CG A:Asp97 2.50 Co CA A:Asp97 4.82 Co O A:Val98 4.54 Co N A:Val98 4.88 Co OG1 A:Thr99 3.87 Co CB A:Asp108 4.31 Co OD2 A:Asp108 2.03 Co C A:Asp108 4.61 Co OD1 A:Asp108 3.24 Co CG A:Asp108 2.98 Co CA A:Asp108 4.74 Co O A:Thr109 4.61 Co N A:Thr109 4.34 Co C A:Thr109 4.79 Co CA A:Thr109 4.95 Co OE1 A:Glu204 4.59 Co OE2 A:Glu204 4.78 Co OE1 A:Glu235 3.37 Co CB A:Glu235 4.92 Co OE2 A:Glu235 2.12 Co CD A:Glu235 3.10 Co CG A:Glu235 4.42 Co CO A:Co401 3.38 Co O A:Mf3300 2.16 Co N A:Mf3300 2.30 Co CB A:Mf3300 4.40 Co C A:Mf3300 2.89 Co CA A:Mf3300 2.88 Co OXT A:Mf3300 4.11 Co O A:Hoh504 3.84 Co O A:Hoh557 4.61 Co O A:Hoh570 4.43 Co O A:Hoh603 4.50 interactive model: