Cobalt in the structure of E. Coli Methionine Aminopeptidase: Methionine Phosphonate Complex (pdb 1c23)

The binding sites of Cobalt atom in the structure of E. Coli Methionine Aminopeptidase: Methionine Phosphonate Complex (pdb code 1c23). This binding sites where shown with 5.0 Angstroms radius around Cobalt atom. The 1c23 structure was solved by W.T.LOWTHER, Y.ZHANG, P.B.SAMPSON, J.F.HONEK, B.W.MATTHEWS, with X-Ray Crystallography technique, brief refinement statistics is given in the table below: Resolution (A) 32.2-2.0 Space group P1211 a (A) 39.221 b (A) 67.518 c (A) 48.823 alpha (°) 90.00 beta (°) 111.20 gamma (°) 90.00 Rfactor (%) n/a Rfree (%) n/a Cobalt Binding Sites: Cobalt binding site 1 out of 2 in 1c23 Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 1 of Cobalt in the PDB 1c23. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Cobalt atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: A: Asp108, A: His171, A: Phe177, A: His178, A: Thr202, A: Glu204, A: Glu235, A: Co402, A: Mph300, A: Hoh582, conact list: Atom Atom Distance (A) Co CB A:Asp108 4.06 Co OD2 A:Asp108 3.46 Co OD1 A:Asp108 1.85 Co CG A:Asp108 2.86 Co NE2 A:His171 2.10 Co ND1 A:His171 4.23 Co CD2 A:His171 3.12 Co CE1 A:His171 3.06 Co CG A:His171 4.27 Co CE1 A:Phe177 4.91 Co NE2 A:His178 4.37 Co CD2 A:His178 4.81 Co CB A:Thr202 4.43 Co CG2 A:Thr202 4.19 Co OG1 A:Thr202 3.87 Co OE1 A:Glu204 3.41 Co OE2 A:Glu204 2.37 Co CD A:Glu204 3.24 Co CG A:Glu204 4.55 Co OE1 A:Glu235 2.07 Co OE2 A:Glu235 3.45 Co CD A:Glu235 3.08 Co CG A:Glu235 4.46 Co CO A:Co402 3.27 Co O2 A:Mph300 2.49 Co N A:Mph300 3.95 Co P A:Mph300 2.75 Co O1 A:Mph300 2.10 Co CA A:Mph300 4.01 Co O3 A:Mph300 3.83 Co O A:Hoh582 4.72 interactive model: Cobalt binding site 2 out of 2 in 1c23 Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 2 of Cobalt in the PDB 1c23. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Cobalt atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: A: Asp97, A: Val98, A: Thr99, A: Asp108, A: Thr109, A: Glu204, A: Glu235, A: Co401, A: Mph300, A: Hoh502, A: Hoh505, A: Hoh583, conact list: Atom Atom Distance (A) Co CB A:Asp97 4.03 Co OD2 A:Asp97 2.36 Co C A:Asp97 4.97 Co OD1 A:Asp97 2.10 Co CG A:Asp97 2.52 Co CA A:Asp97 4.86 Co O A:Val98 4.72 Co N A:Val98 5.00 Co OG1 A:Thr99 3.97 Co CB A:Asp108 4.30 Co OD2 A:Asp108 2.06 Co C A:Asp108 4.70 Co OD1 A:Asp108 3.16 Co CG A:Asp108 2.95 Co CA A:Asp108 4.79 Co O A:Thr109 4.63 Co N A:Thr109 4.41 Co C A:Thr109 4.83 Co OE1 A:Glu204 4.14 Co OE2 A:Glu204 4.80 Co CD A:Glu204 4.80 Co OE1 A:Glu235 3.33 Co CB A:Glu235 4.91 Co OE2 A:Glu235 2.17 Co CD A:Glu235 3.12 Co CG A:Glu235 4.46 Co CO A:Co401 3.27 Co O2 A:Mph300 4.05 Co N A:Mph300 2.26 Co P A:Mph300 3.15 Co CB A:Mph300 4.46 Co O1 A:Mph300 2.13 Co CA A:Mph300 2.98 Co O3 A:Mph300 4.35 Co O A:Hoh502 3.95 Co O A:Hoh505 4.15 Co O A:Hoh583 4.32 interactive model: