Cobalt in the structure of Glutamate Mutase From Clostridium Cochlearium Reconstituted With Methyl-Cobalamin (pdb 1cb7)

The binding sites of Cobalt atom in the structure of Glutamate Mutase From Clostridium Cochlearium Reconstituted With Methyl-Cobalamin (pdb code 1cb7). This binding sites where shown with 5.0 Angstroms radius around Cobalt atom. The 1cb7 structure was solved by K.GRUBER, R.REITZER, C.KRATKY, with X-Ray Crystallography technique, brief refinement statistics is given in the table below: Resolution (A) 25.0-2.0 Space group P1211 a (A) 64.330 b (A) 113.140 c (A) 108.610 alpha (°) 90.00 beta (°) 95.62 gamma (°) 90.00 Rfactor (%) 16 Rfree (%) 21.8 Cobalt Binding Sites: Cobalt binding site 1 out of 2 in 1cb7 Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 1 of Cobalt in the PDB 1cb7. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Cobalt atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: A: His16, A: Cob800, A: Hoh863, B: Hoh942, conact list: Atom Atom Distance (A) Co NE2 A:His16 2.34 Co ND1 A:His16 4.40 Co CD2 A:His16 3.40 Co CE1 A:His16 3.23 Co CG A:His16 4.51 Co C18 A:Cob800 4.11 Co C11 A:Cob800 2.89 Co C1 A:Cob800 2.88 Co C8 A:Cob800 4.17 Co C2 A:Cob800 4.12 Co C46 A:Cob800 4.85 Co N22 A:Cob800 1.91 Co C35 A:Cob800 4.86 Co C17 A:Cob800 4.20 Co N21 A:Cob800 1.88 Co C12 A:Cob800 4.21 Co C19 A:Cob800 2.80 Co C41 A:Cob800 4.94 Co CO A:Cob800 0.00 Co C15 A:Cob800 3.36 Co C37 A:Cob800 4.62 Co C16 A:Cob800 2.94 Co C6 A:Cob800 2.99 Co C5 A:Cob800 3.34 Co O39 A:Cob800 4.69 Co C4 A:Cob800 2.89 Co C10 A:Cob800 3.23 Co C13 A:Cob800 4.19 Co C20 A:Cob800 3.58 Co C48 A:Cob800 4.76 Co C9 A:Cob800 2.85 Co C54 A:Cob800 4.90 Co C14 A:Cob800 2.93 Co N24 A:Cob800 1.88 Co N23 A:Cob800 1.91 Co C7 A:Cob800 4.21 Co C53 A:Cob800 4.85 Co C3 A:Cob800 4.15 Co C26 A:Cob800 4.49 Co C1A A:Cob800 1.96 Co C1A A:Cob800 3.37 Co O A:Hoh863 4.42 Co O B:Hoh942 4.88 interactive model: Cobalt binding site 2 out of 2 in 1cb7 Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 2 of Cobalt in the PDB 1cb7. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Cobalt atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: C: His16, C: Cob800, C: Hoh826, D: Hoh927, conact list: Atom Atom Distance (A) Co NE2 C:His16 2.34 Co ND1 C:His16 4.40 Co CD2 C:His16 3.39 Co CE1 C:His16 3.23 Co CG C:His16 4.52 Co C18 C:Cob800 4.12 Co C11 C:Cob800 2.87 Co C1 C:Cob800 2.90 Co C8 C:Cob800 4.15 Co C2 C:Cob800 4.12 Co C46 C:Cob800 4.82 Co N22 C:Cob800 1.90 Co C35 C:Cob800 4.85 Co C17 C:Cob800 4.21 Co N21 C:Cob800 1.89 Co C12 C:Cob800 4.19 Co C19 C:Cob800 2.81 Co C41 C:Cob800 4.93 Co CO C:Cob800 0.00 Co C15 C:Cob800 3.35 Co C37 C:Cob800 4.57 Co C38 C:Cob800 4.92 Co C16 C:Cob800 2.94 Co C6 C:Cob800 2.98 Co C5 C:Cob800 3.34 Co O39 C:Cob800 4.58 Co C4 C:Cob800 2.90 Co C10 C:Cob800 3.22 Co C13 C:Cob800 4.17 Co C20 C:Cob800 3.58 Co C48 C:Cob800 4.78 Co C9 C:Cob800 2.84 Co C54 C:Cob800 4.89 Co C14 C:Cob800 2.91 Co N24 C:Cob800 1.89 Co N23 C:Cob800 1.90 Co C7 C:Cob800 4.20 Co C53 C:Cob800 4.84 Co C3 C:Cob800 4.16 Co C26 C:Cob800 4.51 Co C1A C:Cob800 1.96 Co C1A C:Cob800 3.37 Co O C:Hoh826 4.66 Co O D:Hoh927 4.94 interactive model: