Cobalt in the structure of Replacement Of Catalytic Histidine-195 of Chloramphenicol Acetyltransferase: Evidence For A General Base Role For Glutamate (pdb 1cia)

The binding sites of Cobalt atom in the structure of Replacement Of Catalytic Histidine-195 of Chloramphenicol Acetyltransferase: Evidence For A General Base Role For Glutamate (pdb code 1cia). This binding sites where shown with 5.0 Angstroms radius around Cobalt atom. The 1cia structure was solved by A.G.W.LESLIE, M.R.GIBBS, with X-Ray Crystallography technique, brief refinement statistics is given in the table below: Resolution (A) 6.0-2.5 Space group H32 a (A) 107.640 b (A) 107.640 c (A) 124.130 alpha (°) 90.00 beta (°) 90.00 gamma (°) 120.00 Rfactor (%) n/a Rfree (%) n/a Cobalt Binding Sites: Cobalt binding site 1 out of 2 in 1cia Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 1 of Cobalt in the PDB 1cia. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Cobalt atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: A: Glu23, A: His27, A: Hoh256, A: Hoh267, A: Hoh268, conact list: Atom Atom Distance (A) Co OE1 A:Glu23 2.71 Co OE2 A:Glu23 1.88 Co CD A:Glu23 2.63 Co CG A:Glu23 4.06 Co NE2 A:His27 3.99 Co CB A:His27 3.49 Co ND1 A:His27 1.98 Co CD2 A:His27 4.10 Co CE1 A:His27 2.88 Co CG A:His27 3.05 Co CA A:His27 4.98 Co O A:Hoh256 4.36 Co O A:Hoh267 4.08 Co O A:Hoh268 4.45 interactive model: Cobalt binding site 2 out of 2 in 1cia Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 2 of Cobalt in the PDB 1cia. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Cobalt atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: A: Asn68, A: Asp87, A: Hoh341, conact list: Atom Atom Distance (A) Co O A:Asn68 4.01 Co O A:Asp87 4.33 Co O A:Hoh341 1.36 interactive model: