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Cobalt in PDB 1e1c: Methylmalonyl-Coa Mutase H244A Mutant

Enzymatic activity of Methylmalonyl-Coa Mutase H244A Mutant

All present enzymatic activity of Methylmalonyl-Coa Mutase H244A Mutant:
5.4.99.2;

Protein crystallography data

The structure of Methylmalonyl-Coa Mutase H244A Mutant, PDB code: 1e1c was solved by P.R.Evans, N.H.Thoma, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 29.20 / 2.62
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 125.398, 161.829, 166.949, 90.00, 90.00, 90.00
R / Rfree (%) 20.7 / 28.3

Cobalt Binding Sites:

The binding sites of Cobalt atom in the Methylmalonyl-Coa Mutase H244A Mutant (pdb code 1e1c). This binding sites where shown within 5.0 Angstroms radius around Cobalt atom.
In total 2 binding sites of Cobalt where determined in the Methylmalonyl-Coa Mutase H244A Mutant, PDB code: 1e1c:
Jump to Cobalt binding site number: 1; 2;

Cobalt binding site 1 out of 2 in 1e1c

Go back to Cobalt Binding Sites List in 1e1c
Cobalt binding site 1 out of 2 in the Methylmalonyl-Coa Mutase H244A Mutant


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 1 of Methylmalonyl-Coa Mutase H244A Mutant within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Co800

b:32.2
occ:1.00
CO A:B12800 0.0 32.2 1.0
N23 A:B12800 1.6 17.7 1.0
N22 A:B12800 1.8 20.2 1.0
N24 A:B12800 1.8 25.8 1.0
N21 A:B12800 2.0 21.3 1.0
NE2 A:HIS610 2.3 28.4 1.0
C11 A:B12800 2.6 16.2 1.0
C9 A:B12800 2.7 24.9 1.0
C14 A:B12800 2.7 18.9 1.0
C16 A:B12800 2.8 23.7 1.0
C19 A:B12800 2.9 28.6 1.0
C1 A:B12800 3.0 29.1 1.0
C6 A:B12800 3.0 19.4 1.0
C10 A:B12800 3.0 21.2 1.0
C4 A:B12800 3.0 20.0 1.0
CD2 A:HIS610 3.2 31.0 1.0
C15 A:B12800 3.3 19.9 1.0
CE1 A:HIS610 3.4 29.2 1.0
C5 A:B12800 3.5 20.9 1.0
C20 A:B12800 3.6 28.2 1.0
C12 A:B12800 3.9 20.4 1.0
C13 A:B12800 4.0 15.0 1.0
C8 A:B12800 4.1 23.2 1.0
C18 A:B12800 4.1 27.3 1.0
C17 A:B12800 4.2 26.4 1.0
C2 A:B12800 4.2 27.1 1.0
C7 A:B12800 4.2 24.6 1.0
C3 A:B12800 4.3 24.1 1.0
CG A:HIS610 4.4 35.0 1.0
ND1 A:HIS610 4.4 36.8 1.0
C26 A:B12800 4.4 29.0 1.0
C54 A:B12800 4.6 23.2 1.0
C48 A:B12800 4.6 17.3 1.0
C42 A:B12800 4.7 33.1 1.0
C46 A:B12800 4.7 18.3 1.0
C53 A:B12800 4.8 23.5 1.0
O A:HOH2263 5.0 47.8 1.0

Cobalt binding site 2 out of 2 in 1e1c

Go back to Cobalt Binding Sites List in 1e1c
Cobalt binding site 2 out of 2 in the Methylmalonyl-Coa Mutase H244A Mutant


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 2 of Methylmalonyl-Coa Mutase H244A Mutant within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Co800

b:35.6
occ:1.00
CO C:B12800 0.0 35.6 1.0
N23 C:B12800 1.7 24.2 1.0
N22 C:B12800 1.7 23.2 1.0
N24 C:B12800 1.9 23.6 1.0
N21 C:B12800 1.9 18.5 1.0
NE2 C:HIS610 2.3 32.0 1.0
C9 C:B12800 2.6 25.1 1.0
C11 C:B12800 2.6 27.6 1.0
O C:HOH2475 2.7 49.2 1.0
C19 C:B12800 2.9 23.2 1.0
C14 C:B12800 2.9 24.3 1.0
C16 C:B12800 2.9 21.8 1.0
C1 C:B12800 2.9 25.1 1.0
C4 C:B12800 2.9 20.0 1.0
C10 C:B12800 2.9 23.5 1.0
C6 C:B12800 2.9 24.9 1.0
CE1 C:HIS610 3.2 32.7 1.0
CD2 C:HIS610 3.4 32.6 1.0
C5 C:B12800 3.4 24.1 1.0
C15 C:B12800 3.4 22.5 1.0
C20 C:B12800 3.7 29.6 1.0
C8 C:B12800 4.0 28.0 1.0
C12 C:B12800 4.0 27.0 1.0
C18 C:B12800 4.1 25.9 1.0
C13 C:B12800 4.1 24.4 1.0
C2 C:B12800 4.1 22.6 1.0
C7 C:B12800 4.2 25.6 1.0
C3 C:B12800 4.2 20.4 1.0
C17 C:B12800 4.2 27.4 1.0
ND1 C:HIS610 4.3 39.2 1.0
C26 C:B12800 4.4 25.5 1.0
CG C:HIS610 4.4 38.2 1.0
C42 C:B12800 4.5 36.8 1.0
C54 C:B12800 4.6 19.5 1.0
C46 C:B12800 4.7 27.7 1.0
C48 C:B12800 4.8 29.9 1.0
C41 C:B12800 4.9 32.2 1.0
C53 C:B12800 4.9 24.5 1.0
C35 C:B12800 4.9 23.4 1.0

Reference:

N.H.Thoma, P.R.Evans, P.F.Leadlay. Protection of Radical Intermediates at the Active Site of Adenosylcobalamin-Dependent Methylmalonyl-Coa Mutase Biochemistry V. 39 9213 2000.
ISSN: ISSN 0006-2960
PubMed: 10924114
DOI: 10.1021/BI0004302
Page generated: Sun Jul 13 17:27:26 2025

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