Cobalt in PDB 1ire: Crystal Structure of Co-Type Nitrile Hydratase From Pseudonocardia Thermophila

All present enzymatic activity of Crystal Structure of Co-Type Nitrile Hydratase From Pseudonocardia Thermophila:
4.2.1.84;

The structure of Crystal Structure of Co-Type Nitrile Hydratase From Pseudonocardia Thermophila, PDB code: 1ire was solved by A.Miyanaga, S.Fushinobu, K.Ito, T.Wakagi, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	28.03 / 1.80
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	65.501, 65.501, 184.614, 90.00, 90.00, 120.00
R / Rfree (%)	17.8 / 19.1

The binding sites of Cobalt atom in the Crystal Structure of Co-Type Nitrile Hydratase From Pseudonocardia Thermophila (pdb code 1ire). This binding sites where shown within 5.0 Angstroms radius around Cobalt atom.
In total only one binding site of Cobalt was determined in the Crystal Structure of Co-Type Nitrile Hydratase From Pseudonocardia Thermophila, PDB code: 1ire:

Cobalt binding site 1 out of 1 in the Crystal Structure of Co-Type Nitrile Hydratase From Pseudonocardia Thermophila


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 1 of Crystal Structure of Co-Type Nitrile Hydratase From Pseudonocardia Thermophila within 5.0Å range: probe atom residue distance (Å) B Occ A:Co301 b:13.7 occ:1.00 N A:CSO113 2.0 12.0 1.0 N A:SER112 2.1 10.3 1.0 SG A:CSD111 2.1 12.6 1.0 SG A:CYS108 2.3 12.2 1.0 SG A:CSO113 2.3 14.8 1.0 O A:HOH432 2.6 26.7 1.0 C A:SER112 2.8 12.3 1.0 CA A:SER112 2.9 11.7 1.0 CA A:CSO113 2.9 13.1 1.0 OD1 A:CSD111 3.0 14.1 1.0 OD A:CSO113 3.0 15.6 1.0 OD2 A:CSD111 3.0 14.7 1.0 CB A:CSO113 3.0 14.3 1.0 CB A:CSD111 3.1 12.4 1.0 C A:CSD111 3.1 11.4 1.0 CB A:CYS108 3.3 11.2 1.0 CA A:CSD111 3.4 9.9 1.0 N A:CSD111 3.8 11.0 1.0 OG A:SER112 3.9 18.1 1.0 O A:SER112 4.0 11.8 1.0 CB A:SER112 4.0 12.9 1.0 O A:CSD111 4.2 11.2 1.0 C A:CSO113 4.3 12.8 1.0 O A:CSO113 4.6 11.8 1.0 CA A:CYS108 4.7 10.7 1.0 NH2 B:ARG157 4.8 10.6 1.0 C A:LEU110 4.9 10.4 1.0 O A:CYS108 4.9 11.8 1.0

A.Miyanaga, S.Fushinobu, K.Ito, T.Wakagi. Crystal Structure of Cobalt-Containing Nitrile Hydratase. Biochem.Biophys.Res.Commun. V. 288 1169 2001.
ISSN: ISSN 0006-291X
PubMed: 11700034
DOI: 10.1006/BBRC.2001.5897