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Cobalt in PDB 1qca: Quadruple Mutant Q92C, N146F, Y168F, I172V Type III Cat Complexed with Fusidic Acid. Crystals Grown at pH 6.3. X-Ray Data Collected at Room Temperature

Enzymatic activity of Quadruple Mutant Q92C, N146F, Y168F, I172V Type III Cat Complexed with Fusidic Acid. Crystals Grown at pH 6.3. X-Ray Data Collected at Room Temperature

All present enzymatic activity of Quadruple Mutant Q92C, N146F, Y168F, I172V Type III Cat Complexed with Fusidic Acid. Crystals Grown at pH 6.3. X-Ray Data Collected at Room Temperature:
2.3.1.28;

Protein crystallography data

The structure of Quadruple Mutant Q92C, N146F, Y168F, I172V Type III Cat Complexed with Fusidic Acid. Crystals Grown at pH 6.3. X-Ray Data Collected at Room Temperature, PDB code: 1qca was solved by A.G.W.Leslie, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 6.00 / 2.20
Space group H 3 2
Cell size a, b, c (Å), α, β, γ (°) 107.810, 107.810, 124.130, 90.00, 90.00, 120.00
R / Rfree (%) n/a / n/a

Cobalt Binding Sites:

The binding sites of Cobalt atom in the Quadruple Mutant Q92C, N146F, Y168F, I172V Type III Cat Complexed with Fusidic Acid. Crystals Grown at pH 6.3. X-Ray Data Collected at Room Temperature (pdb code 1qca). This binding sites where shown within 5.0 Angstroms radius around Cobalt atom.
In total 2 binding sites of Cobalt where determined in the Quadruple Mutant Q92C, N146F, Y168F, I172V Type III Cat Complexed with Fusidic Acid. Crystals Grown at pH 6.3. X-Ray Data Collected at Room Temperature, PDB code: 1qca:
Jump to Cobalt binding site number: 1; 2;

Cobalt binding site 1 out of 2 in 1qca

Go back to Cobalt Binding Sites List in 1qca
Cobalt binding site 1 out of 2 in the Quadruple Mutant Q92C, N146F, Y168F, I172V Type III Cat Complexed with Fusidic Acid. Crystals Grown at pH 6.3. X-Ray Data Collected at Room Temperature


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 1 of Quadruple Mutant Q92C, N146F, Y168F, I172V Type III Cat Complexed with Fusidic Acid. Crystals Grown at pH 6.3. X-Ray Data Collected at Room Temperature within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Co222

b:11.4
occ:0.50
 OE2 A:GLU23 1.9 15.8 1.0
ND1 A:HIS27 2.1 19.4 1.0
CD A:GLU23 2.7 15.0 1.0
OE1 A:GLU23 2.8 12.7 1.0
CE1 A:HIS27 3.1 22.8 1.0
CG A:HIS27 3.2 11.6 1.0
CB A:HIS27 3.6 9.1 1.0
CG A:GLU23 4.1 9.8 1.0
NE2 A:HIS27 4.1 9.6 1.0
CD2 A:HIS27 4.2 11.1 1.0
O A:HOH263 4.3 32.5 1.0
O A:HOH253 4.3 21.8 1.0

Cobalt binding site 2 out of 2 in 1qca

Go back to Cobalt Binding Sites List in 1qca
Cobalt binding site 2 out of 2 in the Quadruple Mutant Q92C, N146F, Y168F, I172V Type III Cat Complexed with Fusidic Acid. Crystals Grown at pH 6.3. X-Ray Data Collected at Room Temperature


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 2 of Quadruple Mutant Q92C, N146F, Y168F, I172V Type III Cat Complexed with Fusidic Acid. Crystals Grown at pH 6.3. X-Ray Data Collected at Room Temperature within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Co223

b:44.3
occ:0.50
 O A:HOH328 1.6 48.4 0.5
O A:HOH277 2.5 41.7 1.0
O A:ASN68 4.1 16.4 1.0
O A:HOH295 4.1 45.8 1.0
O A:ASP87 4.3 18.9 1.0

Reference:

I.A.Murray, P.A.Cann, P.J.Day, J.P.Derrick, M.J.Sutcliffe, W.V.Shaw, A.G.Leslie. Steroid Recognition By Chloramphenicol Acetyltransferase: Engineering and Structural Analysis of A High Affinity Fusidic Acid Binding Site. J.Mol.Biol. V. 254 993 1995.
ISSN: ISSN 0022-2836
PubMed: 7500366
DOI: 10.1006/JMBI.1995.0671
Page generated: Sun Dec 13 10:35:40 2020

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