Cobalt in PDB 1qca: Quadruple Mutant Q92C, N146F, Y168F, I172V Type III Cat Complexed with Fusidic Acid. Crystals Grown at pH 6.3. X-Ray Data Collected at Room Temperature

Enzymatic activity of Quadruple Mutant Q92C, N146F, Y168F, I172V Type III Cat Complexed with Fusidic Acid. Crystals Grown at pH 6.3. X-Ray Data Collected at Room Temperature

All present enzymatic activity of Quadruple Mutant Q92C, N146F, Y168F, I172V Type III Cat Complexed with Fusidic Acid. Crystals Grown at pH 6.3. X-Ray Data Collected at Room Temperature:
2.3.1.28;

Protein crystallography data

The structure of Quadruple Mutant Q92C, N146F, Y168F, I172V Type III Cat Complexed with Fusidic Acid. Crystals Grown at pH 6.3. X-Ray Data Collected at Room Temperature, PDB code: 1qca was solved by A.G.W.Leslie, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

*Resolution Low / High (Å)*	6.00 / 2.20
*Space group*	H 3 2
*Cell size a, b, c (Å), α, β, γ (°)*	107.810, 107.810, 124.130, 90.00, 90.00, 120.00
*R / R_free (%)*	n/a / n/a

Cobalt Binding Sites:

The binding sites of Cobalt atom in the Quadruple Mutant Q92C, N146F, Y168F, I172V Type III Cat Complexed with Fusidic Acid. Crystals Grown at pH 6.3. X-Ray Data Collected at Room Temperature (pdb code 1qca). This binding sites where shown within 5.0 Angstroms radius around Cobalt atom.
In total 2 binding sites of Cobalt where determined in the Quadruple Mutant Q92C, N146F, Y168F, I172V Type III Cat Complexed with Fusidic Acid. Crystals Grown at pH 6.3. X-Ray Data Collected at Room Temperature, PDB code: 1qca:
Jump to Cobalt binding site number: 1; 2;

Cobalt binding site 1 out of 2 in 1qca

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Cobalt Binding Sites List in 1qca

Cobalt binding site 1 out of 2 in the Quadruple Mutant Q92C, N146F, Y168F, I172V Type III Cat Complexed with Fusidic Acid. Crystals Grown at pH 6.3. X-Ray Data Collected at Room Temperature

Mono view

Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 1 of Quadruple Mutant Q92C, N146F, Y168F, I172V Type III Cat Complexed with Fusidic Acid. Crystals Grown at pH 6.3. X-Ray Data Collected at Room Temperature within 5.0Å range:

probe	atom	residue	distance (Å)	B	Occ
A:Co222 b:11.4 occ:0.50	OE2	A:GLU23	1.9	15.8	1.0
	ND1	A:HIS27	2.1	19.4	1.0
	CD	A:GLU23	2.7	15.0	1.0
	OE1	A:GLU23	2.8	12.7	1.0
	CE1	A:HIS27	3.1	22.8	1.0
	CG	A:HIS27	3.2	11.6	1.0
	CB	A:HIS27	3.6	9.1	1.0
	CG	A:GLU23	4.1	9.8	1.0
	NE2	A:HIS27	4.1	9.6	1.0
	CD2	A:HIS27	4.2	11.1	1.0
	O	A:HOH263	4.3	32.5	1.0
	O	A:HOH253	4.3	21.8	1.0

Cobalt binding site 2 out of 2 in 1qca

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Cobalt Binding Sites List in 1qca

Cobalt binding site 2 out of 2 in the Quadruple Mutant Q92C, N146F, Y168F, I172V Type III Cat Complexed with Fusidic Acid. Crystals Grown at pH 6.3. X-Ray Data Collected at Room Temperature

Mono view

Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 2 of Quadruple Mutant Q92C, N146F, Y168F, I172V Type III Cat Complexed with Fusidic Acid. Crystals Grown at pH 6.3. X-Ray Data Collected at Room Temperature within 5.0Å range:

probe	atom	residue	distance (Å)	B	Occ
A:Co223 b:44.3 occ:0.50	O	A:HOH328	1.6	48.4	0.5
	O	A:HOH277	2.5	41.7	1.0
	O	A:ASN68	4.1	16.4	1.0
	O	A:HOH295	4.1	45.8	1.0
	O	A:ASP87	4.3	18.9	1.0

Reference:

I.A.Murray, P.A.Cann, P.J.Day, J.P.Derrick, M.J.Sutcliffe, W.V.Shaw, A.G.Leslie. Steroid Recognition By Chloramphenicol Acetyltransferase: Engineering and Structural Analysis of A High Affinity Fusidic Acid Binding Site. J.Mol.Biol. V. 254 993 1995.
ISSN: ISSN 0022-2836
PubMed: 7500366
DOI: 10.1006/JMBI.1995.0671

Page generated: Tue Jul 30 14:30:57 2024

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