Cobalt in PDB 1zft: The Crystal Structure of An All-Rna Minimal Hairpin Ribozyme with Mutant G8I at the Cleavage Site

The structure of The Crystal Structure of An All-Rna Minimal Hairpin Ribozyme with Mutant G8I at the Cleavage Site, PDB code: 1zft was solved by J.E.Wedekind, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.46 / 2.33
Space group	P 61 2 2
Cell size a, b, c (Å), α, β, γ (°)	93.400, 93.400, 131.500, 90.00, 90.00, 120.00
R / Rfree (%)	22.7 / 24.2

The binding sites of Cobalt atom in the The Crystal Structure of An All-Rna Minimal Hairpin Ribozyme with Mutant G8I at the Cleavage Site (pdb code 1zft). This binding sites where shown within 5.0 Angstroms radius around Cobalt atom.
In total 2 binding sites of Cobalt where determined in the The Crystal Structure of An All-Rna Minimal Hairpin Ribozyme with Mutant G8I at the Cleavage Site, PDB code: 1zft:
Jump to Cobalt binding site number: 1; 2;

Cobalt binding site 1 out of 2 in the The Crystal Structure of An All-Rna Minimal Hairpin Ribozyme with Mutant G8I at the Cleavage Site


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 1 of The Crystal Structure of An All-Rna Minimal Hairpin Ribozyme with Mutant G8I at the Cleavage Site within 5.0Å range: probe atom residue distance (Å) B Occ B:Co102 b:77.3 occ:1.00 CO B:NCO102 0.0 77.3 1.0 N4 B:NCO102 2.0 76.7 1.0 N5 B:NCO102 2.0 77.6 1.0 N3 B:NCO102 2.0 77.8 1.0 N2 B:NCO102 2.0 77.9 1.0 N6 B:NCO102 2.0 77.8 1.0 N1 B:NCO102 2.0 76.8 1.0 O6 B:G12 3.9 46.2 1.0 O6 B:G13 4.0 47.6 1.0 N7 B:G12 4.4 52.0 1.0 O3 A:SO4103 4.5 65.2 0.5 C6 B:G12 4.7 47.1 1.0 C5 B:G12 4.9 49.3 1.0 O2 A:SO4103 4.9 65.0 0.5 N7 B:G13 5.0 48.7 1.0

Cobalt binding site 2 out of 2 in the The Crystal Structure of An All-Rna Minimal Hairpin Ribozyme with Mutant G8I at the Cleavage Site


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 2 of The Crystal Structure of An All-Rna Minimal Hairpin Ribozyme with Mutant G8I at the Cleavage Site within 5.0Å range: probe atom residue distance (Å) B Occ D:Co101 b:30.2 occ:1.00 CO D:NCO101 0.0 30.2 1.0 N5 D:NCO101 1.9 46.8 1.0 N4 D:NCO101 1.9 46.8 1.0 N3 D:NCO101 2.0 48.7 1.0 N2 D:NCO101 2.0 45.5 1.0 N6 D:NCO101 2.0 45.9 1.0 N1 D:NCO101 2.0 44.2 1.0 OP1 D:A40 3.6 69.9 1.0 OP2 D:U41 3.8 60.9 1.0 OP2 C:A20 3.9 74.3 1.0 O6 C:G21 4.1 62.4 1.0 N7 C:G21 4.4 55.8 1.0 P D:A40 4.6 73.1 1.0 OP2 D:A40 4.8 71.9 1.0 C6 C:G21 4.9 57.4 1.0

J.Salter, J.Krucinska, S.Alam, V.Grum-Tokars, J.E.Wedekind. Water in the Active Site of An All-Rna Hairpin Ribozyme and Effects of GUA8 Base Variants on the Geometry of Phosphoryl Transfer. Biochemistry V. 45 686 2006.
ISSN: ISSN 0006-2960
PubMed: 16411744
DOI: 10.1021/BI051887K