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Cobalt in PDB 1zfv: The Structure of An All-Rna Minimal Hairpin Ribozyme with Mutation G8A at the Cleavage Site

Protein crystallography data

The structure of The Structure of An All-Rna Minimal Hairpin Ribozyme with Mutation G8A at the Cleavage Site, PDB code: 1zfv was solved by J.E.Wedekind, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 43.77 / 2.40
Space group P 61 2 2
Cell size a, b, c (Å), α, β, γ (°) 93.510, 93.510, 124.550, 90.00, 90.00, 120.00
R / Rfree (%) 24.3 / 25.3

Cobalt Binding Sites:

The binding sites of Cobalt atom in the The Structure of An All-Rna Minimal Hairpin Ribozyme with Mutation G8A at the Cleavage Site (pdb code 1zfv). This binding sites where shown within 5.0 Angstroms radius around Cobalt atom.
In total only one binding site of Cobalt was determined in the The Structure of An All-Rna Minimal Hairpin Ribozyme with Mutation G8A at the Cleavage Site, PDB code: 1zfv:

Cobalt binding site 1 out of 1 in 1zfv

Go back to Cobalt Binding Sites List in 1zfv
Cobalt binding site 1 out of 1 in the The Structure of An All-Rna Minimal Hairpin Ribozyme with Mutation G8A at the Cleavage Site


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 1 of The Structure of An All-Rna Minimal Hairpin Ribozyme with Mutation G8A at the Cleavage Site within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Co101

b:90.0
occ:1.00
CO D:NCO101 0.0 90.0 1.0
N4 D:NCO101 2.0 90.0 1.0
N5 D:NCO101 2.0 89.5 1.0
N3 D:NCO101 2.0 90.2 1.0
N2 D:NCO101 2.0 90.7 1.0
N6 D:NCO101 2.0 90.7 1.0
N1 D:NCO101 2.0 88.9 1.0
OP1 D:A40 3.5 75.5 1.0
OP2 D:U41 3.8 64.2 1.0
OP2 C:A20 3.8 91.6 1.0
N7 C:G21 4.0 58.9 1.0
O6 C:G21 4.2 61.9 1.0
P D:A40 4.4 74.5 1.0
OP2 D:A40 4.6 75.3 1.0
N7 C:A20 4.7 63.4 1.0
C5 C:G21 4.8 57.7 1.0
C6 C:G21 4.9 59.0 1.0
O5' D:A40 4.9 74.7 1.0

Reference:

J.Salter, J.Krucinska, S.Alam, V.Grum-Tokars, J.E.Wedekind. Water in the Active Site of An All-Rna Hairpin Ribozyme and Effects of GUA8 Base Variants on the Geometry of Phosphoryl Transfer. Biochemistry V. 45 686 2006.
ISSN: ISSN 0006-2960
PubMed: 16411744
DOI: 10.1021/BI051887K
Page generated: Sun Dec 13 10:36:59 2020

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