Cobalt in PDB 2bcz: Crystal Structure of A Minimal, Mutant All-Rna Hairpin Ribozyme (U39C, G8I, 2'Deoxy A-1)

The structure of Crystal Structure of A Minimal, Mutant All-Rna Hairpin Ribozyme (U39C, G8I, 2'Deoxy A-1), PDB code: 2bcz was solved by J.D.Salter, J.E.Wedekind, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	44.30 / 2.40
Space group	P 61 2 2
Cell size a, b, c (Å), α, β, γ (°)	94.490, 94.490, 127.580, 90.00, 90.00, 120.00
R / Rfree (%)	24 / 27

The binding sites of Cobalt atom in the Crystal Structure of A Minimal, Mutant All-Rna Hairpin Ribozyme (U39C, G8I, 2'Deoxy A-1) (pdb code 2bcz). This binding sites where shown within 5.0 Angstroms radius around Cobalt atom.
In total 2 binding sites of Cobalt where determined in the Crystal Structure of A Minimal, Mutant All-Rna Hairpin Ribozyme (U39C, G8I, 2'Deoxy A-1), PDB code: 2bcz:
Jump to Cobalt binding site number: 1; 2;

Cobalt binding site 1 out of 2 in the Crystal Structure of A Minimal, Mutant All-Rna Hairpin Ribozyme (U39C, G8I, 2'Deoxy A-1)


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 1 of Crystal Structure of A Minimal, Mutant All-Rna Hairpin Ribozyme (U39C, G8I, 2'Deoxy A-1) within 5.0Å range: probe atom residue distance (Å) B Occ B:Co14 b:66.8 occ:1.00 CO B:NCO14 0.0 66.8 1.0 N4 B:NCO14 1.9 66.4 1.0 N5 B:NCO14 2.0 71.4 1.0 N3 B:NCO14 2.0 67.8 1.0 N6 B:NCO14 2.0 68.2 1.0 N2 B:NCO14 2.0 67.3 1.0 N1 B:NCO14 2.0 68.1 1.0 O6 B:G12 4.0 54.8 1.0 O6 B:G13 4.1 58.4 1.0 N7 B:G12 4.4 51.0 1.0 C6 B:G12 4.8 52.2 1.0 O3 A:SO421 4.9 67.6 0.5 N7 B:G13 4.9 53.1 1.0 C5 B:G12 5.0 50.6 1.0

Cobalt binding site 2 out of 2 in the Crystal Structure of A Minimal, Mutant All-Rna Hairpin Ribozyme (U39C, G8I, 2'Deoxy A-1)


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 2 of Crystal Structure of A Minimal, Mutant All-Rna Hairpin Ribozyme (U39C, G8I, 2'Deoxy A-1) within 5.0Å range: probe atom residue distance (Å) B Occ D:Co11 b:40.0 occ:1.00 CO D:NCO11 0.0 40.0 1.0 N5 D:NCO11 1.9 42.0 1.0 N4 D:NCO11 1.9 45.9 1.0 N3 D:NCO11 1.9 45.1 1.0 N6 D:NCO11 2.0 46.7 1.0 N2 D:NCO11 2.0 45.0 1.0 N1 D:NCO11 2.0 44.9 1.0 OP1 D:A40 3.7 76.0 1.0 OP2 D:U41 3.7 70.6 1.0 OP2 C:A20 4.0 85.7 1.0 O6 C:G21 4.2 64.5 1.0 N7 C:G21 4.3 64.3 1.0 P D:A40 4.6 78.8 1.0 OP2 D:A40 4.9 76.8 1.0 O5' D:A40 4.9 75.2 1.0 N7 C:A20 5.0 72.4 1.0

J.D.Salter, J.Krucinska, S.Alam, V.Grum-Tokars, J.E.Wedekind. Water in the Active Site of An All-Rna Hairpin Ribozyme and Effects of GUA8 Base Variants on the Geometry of Phosphoryl Transfer. Biochemistry V. 45 686 2006.
ISSN: ISSN 0006-2960
PubMed: 16411744
DOI: 10.1021/BI051887K