Cobalt in PDB 2cla: Crystal Structure of the Asp-199-Asn Mutant of Chloramphenicol Acetyltransferase to 2.35 Angstroms Resolution. Structural Consequences of Disruption of A Buried Salt-Bridge

Enzymatic activity of Crystal Structure of the Asp-199-Asn Mutant of Chloramphenicol Acetyltransferase to 2.35 Angstroms Resolution. Structural Consequences of Disruption of A Buried Salt-Bridge

All present enzymatic activity of Crystal Structure of the Asp-199-Asn Mutant of Chloramphenicol Acetyltransferase to 2.35 Angstroms Resolution. Structural Consequences of Disruption of A Buried Salt-Bridge:
2.3.1.28;

Protein crystallography data

The structure of Crystal Structure of the Asp-199-Asn Mutant of Chloramphenicol Acetyltransferase to 2.35 Angstroms Resolution. Structural Consequences of Disruption of A Buried Salt-Bridge, PDB code: 2cla was solved by M.R.Gibbs, P.C.E.Moody, A.G.W.Leslie, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

*Resolution Low / High (Å)*	N/A / 2.35
*Space group*	H 3 2
*Cell size a, b, c (Å), α, β, γ (°)*	107.700, 107.700, 124.400, 90.00, 90.00, 120.00
*R / R_free (%)*	n/a / n/a

Cobalt Binding Sites:

The binding sites of Cobalt atom in the Crystal Structure of the Asp-199-Asn Mutant of Chloramphenicol Acetyltransferase to 2.35 Angstroms Resolution. Structural Consequences of Disruption of A Buried Salt-Bridge (pdb code 2cla). This binding sites where shown within 5.0 Angstroms radius around Cobalt atom.
In total 2 binding sites of Cobalt where determined in the Crystal Structure of the Asp-199-Asn Mutant of Chloramphenicol Acetyltransferase to 2.35 Angstroms Resolution. Structural Consequences of Disruption of A Buried Salt-Bridge, PDB code: 2cla:
Jump to Cobalt binding site number: 1; 2;

Cobalt binding site 1 out of 2 in 2cla

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Cobalt Binding Sites List in 2cla

Cobalt binding site 1 out of 2 in the Crystal Structure of the Asp-199-Asn Mutant of Chloramphenicol Acetyltransferase to 2.35 Angstroms Resolution. Structural Consequences of Disruption of A Buried Salt-Bridge

Mono view

Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 1 of Crystal Structure of the Asp-199-Asn Mutant of Chloramphenicol Acetyltransferase to 2.35 Angstroms Resolution. Structural Consequences of Disruption of A Buried Salt-Bridge within 5.0Å range:

probe	atom	residue	distance (Å)	B	Occ
A:Co222 b:9.8 occ:0.50	OE1	A:GLU23	1.9	9.8	1.0
	ND1	A:HIS27	2.0	14.5	1.0
	CD	A:GLU23	2.5	15.4	1.0
	OE2	A:GLU23	2.5	14.0	1.0
	CE1	A:HIS27	2.9	11.3	1.0
	CG	A:HIS27	3.2	15.1	1.0
	CB	A:HIS27	3.6	15.3	1.0
	O	A:HOH268	4.0	38.4	1.0
	CG	A:GLU23	4.0	13.6	1.0
	NE2	A:HIS27	4.0	10.2	1.0
	CD2	A:HIS27	4.2	9.5	1.0
	CB	A:GLU23	5.0	14.4	1.0

Cobalt binding site 2 out of 2 in 2cla

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Cobalt Binding Sites List in 2cla

Cobalt binding site 2 out of 2 in the Crystal Structure of the Asp-199-Asn Mutant of Chloramphenicol Acetyltransferase to 2.35 Angstroms Resolution. Structural Consequences of Disruption of A Buried Salt-Bridge

Mono view

Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 2 of Crystal Structure of the Asp-199-Asn Mutant of Chloramphenicol Acetyltransferase to 2.35 Angstroms Resolution. Structural Consequences of Disruption of A Buried Salt-Bridge within 5.0Å range:

probe	atom	residue	distance (Å)	B	Occ
A:Co223 b:33.6 occ:0.50	O	A:HOH282	2.2	27.5	1.0
	O	A:ASN68	3.9	5.7	1.0
	O	A:ASP87	4.1	15.7	1.0
	O	A:HOH486	4.1	42.4	1.0
	O	A:HOH461	4.6	21.7	1.0

Reference:

M.R.Gibbs, P.C.Moody, A.G.Leslie. Crystal Structure of the Aspartic Acid-199----Asparagine Mutant of Chloramphenicol Acetyltransferase to 2.35-A Resolution: Structural Consequences of Disruption of A Buried Salt Bridge. Biochemistry V. 29 11261 1990.
ISSN: ISSN 0006-2960
PubMed: 2271709
DOI: 10.1021/BI00503A015

Page generated: Sun Dec 13 10:37:18 2020

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