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Cobalt in PDB 2cla: Crystal Structure of the Asp-199-Asn Mutant of Chloramphenicol Acetyltransferase to 2.35 Angstroms Resolution. Structural Consequences of Disruption of A Buried Salt-Bridge

Enzymatic activity of Crystal Structure of the Asp-199-Asn Mutant of Chloramphenicol Acetyltransferase to 2.35 Angstroms Resolution. Structural Consequences of Disruption of A Buried Salt-Bridge

All present enzymatic activity of Crystal Structure of the Asp-199-Asn Mutant of Chloramphenicol Acetyltransferase to 2.35 Angstroms Resolution. Structural Consequences of Disruption of A Buried Salt-Bridge:
2.3.1.28;

Protein crystallography data

The structure of Crystal Structure of the Asp-199-Asn Mutant of Chloramphenicol Acetyltransferase to 2.35 Angstroms Resolution. Structural Consequences of Disruption of A Buried Salt-Bridge, PDB code: 2cla was solved by M.R.Gibbs, P.C.E.Moody, A.G.W.Leslie, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) N/A / 2.35
Space group H 3 2
Cell size a, b, c (Å), α, β, γ (°) 107.700, 107.700, 124.400, 90.00, 90.00, 120.00
R / Rfree (%) n/a / n/a

Cobalt Binding Sites:

The binding sites of Cobalt atom in the Crystal Structure of the Asp-199-Asn Mutant of Chloramphenicol Acetyltransferase to 2.35 Angstroms Resolution. Structural Consequences of Disruption of A Buried Salt-Bridge (pdb code 2cla). This binding sites where shown within 5.0 Angstroms radius around Cobalt atom.
In total 2 binding sites of Cobalt where determined in the Crystal Structure of the Asp-199-Asn Mutant of Chloramphenicol Acetyltransferase to 2.35 Angstroms Resolution. Structural Consequences of Disruption of A Buried Salt-Bridge, PDB code: 2cla:
Jump to Cobalt binding site number: 1; 2;

Cobalt binding site 1 out of 2 in 2cla

Go back to Cobalt Binding Sites List in 2cla
Cobalt binding site 1 out of 2 in the Crystal Structure of the Asp-199-Asn Mutant of Chloramphenicol Acetyltransferase to 2.35 Angstroms Resolution. Structural Consequences of Disruption of A Buried Salt-Bridge


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 1 of Crystal Structure of the Asp-199-Asn Mutant of Chloramphenicol Acetyltransferase to 2.35 Angstroms Resolution. Structural Consequences of Disruption of A Buried Salt-Bridge within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Co222

b:9.8
occ:0.50
 OE1 A:GLU23 1.9 9.8 1.0
ND1 A:HIS27 2.0 14.5 1.0
CD A:GLU23 2.5 15.4 1.0
OE2 A:GLU23 2.5 14.0 1.0
CE1 A:HIS27 2.9 11.3 1.0
CG A:HIS27 3.2 15.1 1.0
CB A:HIS27 3.6 15.3 1.0
O A:HOH268 4.0 38.4 1.0
CG A:GLU23 4.0 13.6 1.0
NE2 A:HIS27 4.0 10.2 1.0
CD2 A:HIS27 4.2 9.5 1.0
CB A:GLU23 5.0 14.4 1.0

Cobalt binding site 2 out of 2 in 2cla

Go back to Cobalt Binding Sites List in 2cla
Cobalt binding site 2 out of 2 in the Crystal Structure of the Asp-199-Asn Mutant of Chloramphenicol Acetyltransferase to 2.35 Angstroms Resolution. Structural Consequences of Disruption of A Buried Salt-Bridge


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 2 of Crystal Structure of the Asp-199-Asn Mutant of Chloramphenicol Acetyltransferase to 2.35 Angstroms Resolution. Structural Consequences of Disruption of A Buried Salt-Bridge within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Co223

b:33.6
occ:0.50
 O A:HOH282 2.2 27.5 1.0
O A:ASN68 3.9 5.7 1.0
O A:ASP87 4.1 15.7 1.0
O A:HOH486 4.1 42.4 1.0
O A:HOH461 4.6 21.7 1.0

Reference:

M.R.Gibbs, P.C.Moody, A.G.Leslie. Crystal Structure of the Aspartic Acid-199----Asparagine Mutant of Chloramphenicol Acetyltransferase to 2.35-A Resolution: Structural Consequences of Disruption of A Buried Salt Bridge. Biochemistry V. 29 11261 1990.
ISSN: ISSN 0006-2960
PubMed: 2271709
DOI: 10.1021/BI00503A015
Page generated: Tue Jul 30 14:59:25 2024

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