Cobalt in PDB 2oi7: E. Coli Glmu- Complex with Udp-Glcnac, Desulpho-Coa and Glcnac-1-PO4

All present enzymatic activity of E. Coli Glmu- Complex with Udp-Glcnac, Desulpho-Coa and Glcnac-1-PO4:
2.3.1.157; 2.7.7.23;

The structure of E. Coli Glmu- Complex with Udp-Glcnac, Desulpho-Coa and Glcnac-1-PO4, PDB code: 2oi7 was solved by L.R.Olsen, M.W.Vetting, S.L.Roderick, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	27.45 / 2.54
Space group	H 3 2
Cell size a, b, c (Å), α, β, γ (°)	102.969, 102.969, 644.076, 90.00, 90.00, 120.00
R / Rfree (%)	20.9 / 24.7

The structure of E. Coli Glmu- Complex with Udp-Glcnac, Desulpho-Coa and Glcnac-1-PO4 also contains other interesting chemical elements:

Magnesium

(Mg)

1 atom

The binding sites of Cobalt atom in the E. Coli Glmu- Complex with Udp-Glcnac, Desulpho-Coa and Glcnac-1-PO4 (pdb code 2oi7). This binding sites where shown within 5.0 Angstroms radius around Cobalt atom.
In total 3 binding sites of Cobalt where determined in the E. Coli Glmu- Complex with Udp-Glcnac, Desulpho-Coa and Glcnac-1-PO4, PDB code: 2oi7:
Jump to Cobalt binding site number: 1; 2; 3;

Cobalt binding site 1 out of 3 in the E. Coli Glmu- Complex with Udp-Glcnac, Desulpho-Coa and Glcnac-1-PO4


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 1 of E. Coli Glmu- Complex with Udp-Glcnac, Desulpho-Coa and Glcnac-1-PO4 within 5.0Å range: probe atom residue distance (Å) B Occ A:Co6003 b:23.3 occ:0.33 OD2 A:ASP406 2.2 21.5 1.0 CG A:ASP406 3.2 21.3 1.0 OD1 A:ASP406 3.7 22.4 1.0 O A:ASP406 4.1 20.3 1.0 CB A:ASP406 4.2 20.7 1.0 OE1 A:GLN408 4.5 33.3 1.0 O A:HOH8101 4.9 17.7 1.0 C A:ASP406 5.0 19.6 1.0

Cobalt binding site 2 out of 3 in the E. Coli Glmu- Complex with Udp-Glcnac, Desulpho-Coa and Glcnac-1-PO4


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 2 of E. Coli Glmu- Complex with Udp-Glcnac, Desulpho-Coa and Glcnac-1-PO4 within 5.0Å range: probe atom residue distance (Å) B Occ B:Co6001 b:23.3 occ:0.33 OD1 B:ASP406 2.3 14.0 1.0 CO B:CO6002 3.4 23.3 0.3 CG B:ASP406 3.5 18.8 1.0 OD2 B:ASP406 4.3 21.9 1.0 O B:GLY381 4.5 15.1 1.0 CB B:ASP406 4.5 18.4 1.0 CA B:ASP406 4.9 18.6 1.0

Cobalt binding site 3 out of 3 in the E. Coli Glmu- Complex with Udp-Glcnac, Desulpho-Coa and Glcnac-1-PO4


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 3 of E. Coli Glmu- Complex with Udp-Glcnac, Desulpho-Coa and Glcnac-1-PO4 within 5.0Å range: probe atom residue distance (Å) B Occ B:Co6002 b:23.3 occ:0.33 OD2 B:ASP406 2.3 21.9 1.0 OD1 B:ASP406 2.5 14.0 1.0 CG B:ASP406 2.7 18.8 1.0 CO B:CO6001 3.4 23.3 0.3 O B:ASP406 4.2 19.4 1.0 CB B:ASP406 4.2 18.4 1.0 NE2 B:GLN408 4.4 27.8 1.0

L.R.Olsen, M.W.Vetting, S.L.Roderick. Structure of the E. Coli Bifunctional Glmu Acetyltransferase Active Site with Substrates and Products. Protein Sci. V. 16 1230 2007.
ISSN: ISSN 0961-8368
PubMed: 17473010
DOI: 10.1110/PS.072779707