Cobalt in PDB 2uzp: Crystal Structure of the C2 Domain of Human Protein Kinase C Gamma.

All present enzymatic activity of Crystal Structure of the C2 Domain of Human Protein Kinase C Gamma.:
2.7.11.13;

The structure of Crystal Structure of the C2 Domain of Human Protein Kinase C Gamma., PDB code: 2uzp was solved by A.C.W.Pike, A.Amos, C.Johansson, F.Sobott, P.Savitsky, G.Berridge, O.Fedorov, C.Umeano, F.Gorrec, G.Bunkoczi, J.Debreczeni, F.Von Delft, C.H.Arrowsmith, A.Edwards, J.Weigelt, M.Sundstrom, S.Knapp, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 2.00
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	138.503, 138.503, 68.358, 90.00, 90.00, 90.00
R / Rfree (%)	17.1 / 20.5

The structure of Crystal Structure of the C2 Domain of Human Protein Kinase C Gamma. also contains other interesting chemical elements:

Calcium

(Ca)

9 atoms

The binding sites of Cobalt atom in the Crystal Structure of the C2 Domain of Human Protein Kinase C Gamma. (pdb code 2uzp). This binding sites where shown within 5.0 Angstroms radius around Cobalt atom.
In total 3 binding sites of Cobalt where determined in the Crystal Structure of the C2 Domain of Human Protein Kinase C Gamma., PDB code: 2uzp:
Jump to Cobalt binding site number: 1; 2; 3;

Cobalt binding site 1 out of 3 in the Crystal Structure of the C2 Domain of Human Protein Kinase C Gamma.


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 1 of Crystal Structure of the C2 Domain of Human Protein Kinase C Gamma. within 5.0Å range: probe atom residue distance (Å) B Occ A:Co1295 b:23.0 occ:0.80 O3 A:PLP1294 1.8 28.2 1.0 O A:HOH2134 1.8 32.1 1.0 N A:MET0 1.9 36.5 1.0 ND1 A:HIS154 1.9 27.8 1.0 N A:DSN-1 2.0 38.3 1.0 N A:HIS154 2.0 34.0 1.0 C A:DSN-1 2.8 42.4 1.0 C A:MET0 2.8 35.8 1.0 CA A:DSN-1 2.9 42.9 1.0 CA A:MET0 2.9 39.2 1.0 C4A A:PLP1294 2.9 30.9 1.0 CE1 A:HIS154 2.9 32.8 1.0 CG A:HIS154 2.9 33.0 1.0 C3 A:PLP1294 2.9 36.1 1.0 CA A:HIS154 3.1 34.0 1.0 CB A:HIS154 3.3 35.2 1.0 C4 A:PLP1294 3.3 36.6 1.0 C A:HIS154 3.8 36.7 1.0 O A:DSN-1 3.9 39.0 1.0 N A:THR155 4.0 36.8 1.0 CB A:MET0 4.0 38.4 1.0 NE2 A:HIS154 4.0 36.2 1.0 CD2 A:HIS154 4.0 34.5 1.0 CB A:DSN-1 4.0 46.9 1.0 O A:MET0 4.0 37.9 1.0 OG A:DSN-1 4.0 54.9 1.0 CG A:MET0 4.1 40.5 1.0 C2 A:PLP1294 4.1 33.0 1.0 O1 A:SO41304 4.2 26.9 0.8 C2A A:PLP1294 4.7 23.3 1.0 C5 A:PLP1294 4.7 36.2 1.0 O A:HIS154 4.8 36.9 1.0 OG1 A:THR155 4.9 33.9 1.0

Cobalt binding site 2 out of 3 in the Crystal Structure of the C2 Domain of Human Protein Kinase C Gamma.


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 2 of Crystal Structure of the C2 Domain of Human Protein Kinase C Gamma. within 5.0Å range: probe atom residue distance (Å) B Occ B:Co1295 b:25.0 occ:0.80 O3 B:PLP1294 1.8 29.2 1.0 O B:HOH2099 1.8 38.5 1.0 N B:MET0 1.9 32.0 1.0 ND1 B:HIS154 1.9 23.2 1.0 N B:DSN-1 1.9 35.7 1.0 N B:HIS154 2.0 32.5 1.0 C B:DSN-1 2.7 39.4 1.0 C B:MET0 2.8 32.8 1.0 CA B:DSN-1 2.8 38.8 1.0 C4A B:PLP1294 2.9 32.9 1.0 CA B:MET0 2.9 37.8 1.0 CE1 B:HIS154 2.9 32.1 1.0 CG B:HIS154 2.9 30.5 1.0 C3 B:PLP1294 2.9 36.7 1.0 CA B:HIS154 3.1 33.7 1.0 CB B:HIS154 3.3 33.2 1.0 C4 B:PLP1294 3.3 34.7 1.0 C B:HIS154 3.8 34.7 1.0 O B:DSN-1 3.9 38.5 1.0 N B:THR155 3.9 35.6 1.0 CB B:MET0 4.0 36.7 1.0 CB B:DSN-1 4.0 42.8 1.0 NE2 B:HIS154 4.0 28.7 1.0 O B:MET0 4.0 32.4 1.0 OG B:DSN-1 4.0 48.9 1.0 CD2 B:HIS154 4.0 28.7 1.0 CG B:MET0 4.1 43.7 1.0 C2 B:PLP1294 4.1 31.2 1.0 O4 B:SO41302 4.4 35.0 1.0 C2A B:PLP1294 4.6 38.7 1.0 C5 B:PLP1294 4.7 35.1 1.0 O B:HIS154 4.8 33.0 1.0 OG1 B:THR155 5.0 33.3 1.0

Cobalt binding site 3 out of 3 in the Crystal Structure of the C2 Domain of Human Protein Kinase C Gamma.


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 3 of Crystal Structure of the C2 Domain of Human Protein Kinase C Gamma. within 5.0Å range: probe atom residue distance (Å) B Occ C:Co1295 b:22.8 occ:0.80 O C:HOH2114 1.6 27.4 1.0 O3 C:PLP1294 1.8 28.3 1.0 ND1 C:HIS154 1.9 27.2 1.0 N C:MET0 1.9 34.5 1.0 N C:DSN-1 1.9 37.0 1.0 N C:HIS154 2.0 32.2 1.0 C4A C:PLP1294 2.8 29.4 1.0 C C:DSN-1 2.8 38.8 1.0 C C:MET0 2.8 33.9 1.0 CE1 C:HIS154 2.9 36.3 1.0 CA C:DSN-1 2.9 39.6 1.0 CA C:MET0 2.9 37.0 1.0 CG C:HIS154 2.9 36.5 1.0 C3 C:PLP1294 2.9 29.3 1.0 CA C:HIS154 3.1 34.3 1.0 C4 C:PLP1294 3.2 33.3 1.0 CB C:HIS154 3.3 32.8 1.0 C C:HIS154 3.9 34.3 1.0 NE2 C:HIS154 4.0 27.8 1.0 OG C:DSN-1 4.0 48.7 1.0 N C:THR155 4.0 32.9 1.0 O C:DSN-1 4.0 39.7 1.0 CD2 C:HIS154 4.0 34.7 1.0 CB C:DSN-1 4.0 42.2 1.0 O C:MET0 4.0 32.8 1.0 CB C:MET0 4.0 35.4 1.0 CG C:MET0 4.1 39.5 1.0 C2 C:PLP1294 4.1 33.8 1.0 O2 C:SO41301 4.3 38.3 1.0 C5 C:PLP1294 4.6 32.7 1.0 C2A C:PLP1294 4.7 27.3 1.0 O C:HIS154 4.8 34.0 1.0 OG1 C:THR155 4.9 35.9 1.0 NE A:ARG249 4.9 36.6 1.0

A.C.W.Pike, A.Amos, C.Johansson, F.Sobott, P.Savitsky, G.Berridge, O.Fedorov, C.Umeano, F.Gorrec, G.Bunkoczi, J.Debreczeni, F.Von Delft, C.H.Arrowsmith, A.Edwards, J.Weigelt, M.Sundstrom, S.Knapp. Crystal Structure of C2 Domain of Protein Kinase C Gamma To Be Published.