Atomistry » Cobalt » PDB 2r5v-2xwp » 2xin
Atomistry »
  Cobalt »
    PDB 2r5v-2xwp »
      2xin »

Cobalt in PDB 2xin: Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites

Enzymatic activity of Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites

All present enzymatic activity of Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites:
5.3.1.5;

Protein crystallography data

The structure of Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites, PDB code: 2xin was solved by J.Janin, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) N/A / 2.30
Space group P 32 2 1
Cell size a, b, c (Å), α, β, γ (°) 143.450, 143.450, 231.500, 90.00, 90.00, 120.00
R / Rfree (%) n/a / n/a

Cobalt Binding Sites:

The binding sites of Cobalt atom in the Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites (pdb code 2xin). This binding sites where shown within 5.0 Angstroms radius around Cobalt atom.
In total 8 binding sites of Cobalt where determined in the Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites, PDB code: 2xin:
Jump to Cobalt binding site number: 1; 2; 3; 4; 5; 6; 7; 8;

Cobalt binding site 1 out of 8 in 2xin

Go back to Cobalt Binding Sites List in 2xin
Cobalt binding site 1 out of 8 in the Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 1 of Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Co395

b:17.6
occ:1.00
 OE1 A:GLU217 2.0 20.6 1.0
OD2 A:ASP292 2.0 11.9 1.0
OE2 A:GLU181 2.1 14.6 1.0
OD2 A:ASP245 2.1 17.3 1.0
O4 A:SOR397 2.1 17.8 1.0
O2 A:SOR397 2.2 13.7 1.0
CD A:GLU181 3.0 15.4 1.0
CG A:ASP292 3.1 12.2 1.0
CD A:GLU217 3.2 19.6 1.0
C4 A:SOR397 3.2 17.6 1.0
CG A:ASP245 3.3 15.4 1.0
C2 A:SOR397 3.3 15.0 1.0
OE1 A:GLU181 3.3 15.3 1.0
C3 A:SOR397 3.4 15.7 1.0
O3 A:SOR397 3.6 15.4 1.0
CO A:CO396 3.6 20.3 1.0
OE2 A:GLU217 3.7 21.2 1.0
CB A:ASP292 3.8 11.8 1.0
CB A:ASP245 3.8 13.3 1.0
O A:HOH537 4.0 17.6 1.0
O A:HOH453 4.0 15.8 1.0
OD1 A:ASP292 4.1 12.3 1.0
CE1 A:HIS220 4.1 12.2 1.0
CG A:GLU217 4.2 17.4 1.0
OD1 A:ASP245 4.2 14.3 1.0
NE2 A:HIS220 4.3 12.2 1.0
CG A:GLU181 4.4 13.8 1.0
CB A:GLU217 4.4 16.2 1.0
C1 A:SOR397 4.5 13.3 1.0
C5 A:SOR397 4.5 18.1 1.0
ND2 A:ASN215 4.8 11.2 1.0
O1 A:SOR397 4.8 13.7 1.0
O6 A:SOR397 5.0 22.5 1.0
C6 A:SOR397 5.0 20.0 1.0

Cobalt binding site 2 out of 8 in 2xin

Go back to Cobalt Binding Sites List in 2xin
Cobalt binding site 2 out of 8 in the Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 2 of Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Co396

b:20.3
occ:1.00
 OE2 A:GLU217 1.9 21.2 1.0
O A:HOH602 2.1 14.0 1.0
O2 A:SOR397 2.2 13.7 1.0
NE2 A:HIS220 2.2 12.2 1.0
O A:HOH537 2.2 17.6 1.0
O1 A:SOR397 2.2 13.7 1.0
CD A:GLU217 2.8 19.6 1.0
C1 A:SOR397 3.0 13.3 1.0
C2 A:SOR397 3.1 15.0 1.0
CE1 A:HIS220 3.1 12.2 1.0
OE1 A:GLU217 3.1 20.6 1.0
CD2 A:HIS220 3.2 12.6 1.0
CO A:CO395 3.6 17.6 1.0
OD1 A:ASP257 3.7 17.7 1.0
O A:HOH588 3.8 26.7 1.0
OD2 A:ASP292 4.0 11.9 1.0
CG A:GLU217 4.2 17.4 1.0
ND1 A:HIS220 4.2 13.4 1.0
OD1 A:ASP255 4.2 32.5 1.0
CG A:HIS220 4.3 13.0 1.0
OE2 A:GLU181 4.3 14.6 1.0
OD2 A:ASP255 4.3 28.9 1.0
C3 A:SOR397 4.4 15.7 1.0
O3 A:SOR397 4.5 15.4 1.0
ND2 A:ASN247 4.5 10.1 1.0
NZ A:LYS183 4.5 9.1 1.0
CG A:ASP292 4.6 12.2 1.0
CG A:ASP257 4.6 15.1 1.0
OD2 A:ASP257 4.7 17.1 1.0
CG A:ASP255 4.7 26.9 1.0
CE A:LYS183 4.7 5.9 1.0
OD1 A:ASP292 4.7 12.3 1.0
CB A:GLU217 4.8 16.2 1.0
CD A:LYS183 4.9 7.7 1.0

Cobalt binding site 3 out of 8 in 2xin

Go back to Cobalt Binding Sites List in 2xin
Cobalt binding site 3 out of 8 in the Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 3 of Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Co395

b:19.8
occ:1.00
 OE1 B:GLU217 2.1 16.0 1.0
OD2 B:ASP292 2.1 18.1 1.0
O4 B:SOR397 2.2 21.2 1.0
OE2 B:GLU181 2.3 15.3 1.0
OD2 B:ASP245 2.3 16.8 1.0
O2 B:SOR397 2.3 18.8 1.0
CD B:GLU181 3.1 16.5 1.0
CD B:GLU217 3.2 13.6 1.0
CG B:ASP292 3.2 17.4 1.0
OE1 B:GLU181 3.2 16.5 1.0
C2 B:SOR397 3.3 18.4 1.0
C4 B:SOR397 3.3 21.6 1.0
CG B:ASP245 3.3 15.2 1.0
C3 B:SOR397 3.5 19.1 1.0
CO B:CO396 3.6 19.9 1.0
O3 B:SOR397 3.6 20.7 1.0
OE2 B:GLU217 3.7 14.7 1.0
CB B:ASP292 3.8 16.4 1.0
O B:HOH459 3.8 22.9 1.0
CB B:ASP245 3.8 14.0 1.0
O B:HOH543 4.1 20.0 1.0
OD1 B:ASP292 4.2 18.5 1.0
CG B:GLU217 4.2 12.6 1.0
CE1 B:HIS220 4.3 12.2 1.0
OD1 B:ASP245 4.3 15.1 1.0
CB B:GLU217 4.3 10.6 1.0
CG B:GLU181 4.4 14.6 1.0
NE2 B:HIS220 4.5 12.2 1.0
C1 B:SOR397 4.6 17.7 1.0
C5 B:SOR397 4.6 21.9 1.0
ND2 B:ASN215 4.7 15.9 1.0
O1 B:SOR397 4.7 16.2 1.0
O6 B:SOR397 5.0 25.0 1.0

Cobalt binding site 4 out of 8 in 2xin

Go back to Cobalt Binding Sites List in 2xin
Cobalt binding site 4 out of 8 in the Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 4 of Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Co396

b:19.9
occ:1.00
 O2 B:SOR397 2.1 18.8 1.0
OE2 B:GLU217 2.1 14.7 1.0
O B:HOH543 2.2 20.0 1.0
O1 B:SOR397 2.2 16.2 1.0
O B:HOH605 2.2 14.9 1.0
NE2 B:HIS220 2.3 12.2 1.0
CD B:GLU217 2.9 13.6 1.0
CE1 B:HIS220 3.1 12.2 1.0
C1 B:SOR397 3.2 17.7 1.0
C2 B:SOR397 3.2 18.4 1.0
OE1 B:GLU217 3.3 16.0 1.0
CD2 B:HIS220 3.3 12.5 1.0
CO B:CO395 3.6 19.8 1.0
OD1 B:ASP257 3.8 18.2 1.0
O B:HOH592 3.9 25.3 1.0
OD1 B:ASP255 4.0 32.5 1.0
OD2 B:ASP292 4.1 18.1 1.0
ND1 B:HIS220 4.3 12.3 1.0
CG B:GLU217 4.3 12.6 1.0
OE2 B:GLU181 4.3 15.3 1.0
NZ B:LYS183 4.4 11.8 1.0
C3 B:SOR397 4.4 19.1 1.0
CG B:HIS220 4.4 12.5 1.0
O3 B:SOR397 4.4 20.7 1.0
ND2 B:ASN247 4.5 10.7 1.0
CG B:ASP292 4.6 17.4 1.0
CE B:LYS183 4.7 9.2 1.0
OD2 B:ASP257 4.7 19.7 1.0
CG B:ASP257 4.8 16.6 1.0
CB B:GLU217 4.8 10.6 1.0
CD B:LYS183 4.9 11.0 1.0
CG B:ASP255 4.9 28.7 1.0
OD1 B:ASP292 5.0 18.5 1.0

Cobalt binding site 5 out of 8 in 2xin

Go back to Cobalt Binding Sites List in 2xin
Cobalt binding site 5 out of 8 in the Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 5 of Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Co395

b:17.0
occ:1.00
 OD2 C:ASP245 2.0 19.8 1.0
OD2 C:ASP292 2.1 14.0 1.0
OE2 C:GLU181 2.1 13.4 1.0
OE1 C:GLU217 2.2 14.0 1.0
O4 C:SOR397 2.2 17.0 1.0
O2 C:SOR397 2.2 19.1 1.0
CD C:GLU181 3.1 14.6 1.0
CG C:ASP245 3.2 16.8 1.0
C4 C:SOR397 3.2 18.3 1.0
CG C:ASP292 3.2 12.8 1.0
C2 C:SOR397 3.2 19.0 1.0
CD C:GLU217 3.2 13.0 1.0
C3 C:SOR397 3.4 18.2 1.0
OE1 C:GLU181 3.5 16.1 1.0
CO C:CO396 3.6 18.7 1.0
O3 C:SOR397 3.7 19.4 1.0
CB C:ASP292 3.7 12.3 1.0
OE2 C:GLU217 3.8 14.2 1.0
CB C:ASP245 3.8 14.5 1.0
OD1 C:ASP245 4.1 17.8 1.0
CE1 C:HIS220 4.1 10.7 1.0
O C:HOH465 4.1 16.0 1.0
O C:HOH544 4.1 22.0 1.0
OD1 C:ASP292 4.2 11.2 1.0
NE2 C:HIS220 4.3 9.8 1.0
CG C:GLU217 4.3 10.5 1.0
CB C:GLU217 4.4 10.6 1.0
CG C:GLU181 4.4 12.0 1.0
C1 C:SOR397 4.5 17.9 1.0
C5 C:SOR397 4.5 17.8 1.0
ND2 C:ASN215 4.6 10.7 1.0
O1 C:SOR397 4.8 18.3 1.0
O6 C:SOR397 4.8 22.4 1.0
C6 C:SOR397 4.9 20.1 1.0

Cobalt binding site 6 out of 8 in 2xin

Go back to Cobalt Binding Sites List in 2xin
Cobalt binding site 6 out of 8 in the Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 6 of Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Co396

b:18.7
occ:1.00
 O2 C:SOR397 2.0 19.1 1.0
OE2 C:GLU217 2.1 14.2 1.0
NE2 C:HIS220 2.2 9.8 1.0
O C:HOH616 2.2 8.8 1.0
O1 C:SOR397 2.4 18.3 1.0
O C:HOH544 2.4 22.0 1.0
CD C:GLU217 2.9 13.0 1.0
C1 C:SOR397 3.0 17.9 1.0
C2 C:SOR397 3.0 19.0 1.0
CE1 C:HIS220 3.1 10.7 1.0
CD2 C:HIS220 3.2 10.7 1.0
OE1 C:GLU217 3.3 14.0 1.0
CO C:CO395 3.6 17.0 1.0
OD1 C:ASP257 3.7 19.0 1.0
O C:HOH615 3.9 33.2 1.0
OD2 C:ASP292 4.1 14.0 1.0
ND1 C:HIS220 4.2 12.1 1.0
CG C:HIS220 4.3 10.8 1.0
C3 C:SOR397 4.3 18.2 1.0
CG C:GLU217 4.3 10.5 1.0
OD1 C:ASP255 4.3 31.6 1.0
ND2 C:ASN247 4.4 10.5 1.0
OE2 C:GLU181 4.5 13.4 1.0
O3 C:SOR397 4.5 19.4 1.0
CG C:ASP292 4.5 12.8 1.0
OD2 C:ASP255 4.6 31.1 1.0
NZ C:LYS183 4.6 10.7 1.0
OD2 C:ASP257 4.6 19.0 1.0
CG C:ASP257 4.6 17.2 1.0
CB C:GLU217 4.8 10.6 1.0
CE C:LYS183 4.8 9.7 1.0
OD1 C:ASP292 4.8 11.2 1.0
CG C:ASP255 4.9 27.9 1.0
CD C:LYS183 5.0 11.0 1.0

Cobalt binding site 7 out of 8 in 2xin

Go back to Cobalt Binding Sites List in 2xin
Cobalt binding site 7 out of 8 in the Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 7 of Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Co395

b:18.6
occ:1.00
 OE1 D:GLU217 2.0 15.2 1.0
OD2 D:ASP292 2.0 12.5 1.0
O4 D:SOR397 2.1 17.2 1.0
OE2 D:GLU181 2.2 23.4 1.0
OD2 D:ASP245 2.2 18.3 1.0
O2 D:SOR397 2.4 16.4 1.0
CG D:ASP292 3.1 12.4 1.0
CD D:GLU181 3.1 21.4 1.0
CD D:GLU217 3.1 14.9 1.0
C2 D:SOR397 3.2 15.5 1.0
CG D:ASP245 3.3 17.5 1.0
C4 D:SOR397 3.3 17.5 1.0
OE1 D:GLU181 3.3 21.5 1.0
O3 D:SOR397 3.4 15.8 1.0
C3 D:SOR397 3.4 16.0 1.0
CO D:CO396 3.7 23.4 1.0
CB D:ASP292 3.7 11.5 1.0
CB D:ASP245 3.8 16.4 1.0
OE2 D:GLU217 3.8 17.1 1.0
O D:HOH475 3.9 21.4 1.0
O D:HOH553 4.0 19.7 1.0
OD1 D:ASP292 4.1 10.2 1.0
CG D:GLU217 4.2 12.6 1.0
CE1 D:HIS220 4.2 9.3 1.0
OD1 D:ASP245 4.3 19.5 1.0
CB D:GLU217 4.4 12.6 1.0
CG D:GLU181 4.4 17.9 1.0
NE2 D:HIS220 4.5 10.3 1.0
C5 D:SOR397 4.5 17.7 1.0
C1 D:SOR397 4.6 15.5 1.0
ND2 D:ASN215 4.6 13.3 1.0
O1 D:SOR397 4.8 13.9 1.0
O6 D:SOR397 5.0 21.2 1.0

Cobalt binding site 8 out of 8 in 2xin

Go back to Cobalt Binding Sites List in 2xin
Cobalt binding site 8 out of 8 in the Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 8 of Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Co396

b:23.4
occ:1.00
 O2 D:SOR397 1.9 16.4 1.0
O D:HOH553 2.2 19.7 1.0
NE2 D:HIS220 2.2 10.3 1.0
OE2 D:GLU217 2.2 17.1 1.0
O1 D:SOR397 2.3 13.9 1.0
O D:HOH602 2.3 77.0 1.0
CD D:GLU217 2.9 14.9 1.0
CE1 D:HIS220 3.1 9.3 1.0
C2 D:SOR397 3.1 15.5 1.0
OE1 D:GLU217 3.2 15.2 1.0
C1 D:SOR397 3.2 15.5 1.0
CD2 D:HIS220 3.3 9.7 1.0
OD1 D:ASP257 3.6 19.7 1.0
CO D:CO395 3.7 18.6 1.0
OD1 D:ASP255 3.8 32.8 1.0
O D:HOH615 3.9 23.9 1.0
OD2 D:ASP292 4.1 12.5 1.0
ND1 D:HIS220 4.2 9.7 1.0
CG D:GLU217 4.2 12.6 1.0
CG D:HIS220 4.3 10.1 1.0
C3 D:SOR397 4.4 16.0 1.0
OE2 D:GLU181 4.4 23.4 1.0
O3 D:SOR397 4.4 15.8 1.0
ND2 D:ASN247 4.4 6.8 1.0
NZ D:LYS183 4.4 9.6 1.0
CG D:ASP292 4.6 12.4 1.0
CG D:ASP257 4.6 16.2 1.0
OD2 D:ASP257 4.7 16.8 1.0
CB D:GLU217 4.8 12.6 1.0
CG D:ASP255 4.8 28.7 1.0
CE D:LYS183 4.9 8.2 1.0
OD1 D:ASP292 4.9 10.2 1.0

Reference:

J.Jenkins, J.Janin, F.Rey, M.Chiadmi, H.Van Tilbeurgh, I.Lasters, M.De Maeyer, D.Van Belle, S.J.Wodak, M.Lauwereys, P.Stanssens, G.Matthyssens, A.M.Lambeir. Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site-Directed Mutagenesis of Metal Binding Sites. Biochemistry V. 31 5449 1992.
ISSN: ISSN 0006-2960
PubMed: 1610791
DOI: 10.1021/BI00139A005
Page generated: Sun Dec 13 10:39:15 2020

Last articles

Zn in 8WB0
Zn in 8WAX
Zn in 8WAU
Zn in 8WAZ
Zn in 8WAY
Zn in 8WAV
Zn in 8WAW
Zn in 8WAT
Zn in 8W7M
Zn in 8WD3
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy