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Cobalt in PDB 2xin: Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites

Enzymatic activity of Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites

All present enzymatic activity of Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites:
5.3.1.5;

Protein crystallography data

The structure of Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites, PDB code: 2xin was solved by J.Janin, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) N/A / 2.30
Space group P 32 2 1
Cell size a, b, c (Å), α, β, γ (°) 143.450, 143.450, 231.500, 90.00, 90.00, 120.00
R / Rfree (%) n/a / n/a

Cobalt Binding Sites:

The binding sites of Cobalt atom in the Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites (pdb code 2xin). This binding sites where shown within 5.0 Angstroms radius around Cobalt atom.
In total 8 binding sites of Cobalt where determined in the Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites, PDB code: 2xin:
Jump to Cobalt binding site number: 1; 2; 3; 4; 5; 6; 7; 8;

Cobalt binding site 1 out of 8 in 2xin

Go back to Cobalt Binding Sites List in 2xin
Cobalt binding site 1 out of 8 in the Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 1 of Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Co395

b:17.6
occ:1.00
 OE1 A:GLU217 2.0 20.6 1.0
OD2 A:ASP292 2.0 11.9 1.0
OE2 A:GLU181 2.1 14.6 1.0
OD2 A:ASP245 2.1 17.3 1.0
O4 A:SOR397 2.1 17.8 1.0
O2 A:SOR397 2.2 13.7 1.0
CD A:GLU181 3.0 15.4 1.0
CG A:ASP292 3.1 12.2 1.0
CD A:GLU217 3.2 19.6 1.0
C4 A:SOR397 3.2 17.6 1.0
CG A:ASP245 3.3 15.4 1.0
C2 A:SOR397 3.3 15.0 1.0
OE1 A:GLU181 3.3 15.3 1.0
C3 A:SOR397 3.4 15.7 1.0
O3 A:SOR397 3.6 15.4 1.0
CO A:CO396 3.6 20.3 1.0
OE2 A:GLU217 3.7 21.2 1.0
CB A:ASP292 3.8 11.8 1.0
CB A:ASP245 3.8 13.3 1.0
O A:HOH537 4.0 17.6 1.0
O A:HOH453 4.0 15.8 1.0
OD1 A:ASP292 4.1 12.3 1.0
CE1 A:HIS220 4.1 12.2 1.0
CG A:GLU217 4.2 17.4 1.0
OD1 A:ASP245 4.2 14.3 1.0
NE2 A:HIS220 4.3 12.2 1.0
CG A:GLU181 4.4 13.8 1.0
CB A:GLU217 4.4 16.2 1.0
C1 A:SOR397 4.5 13.3 1.0
C5 A:SOR397 4.5 18.1 1.0
ND2 A:ASN215 4.8 11.2 1.0
O1 A:SOR397 4.8 13.7 1.0
O6 A:SOR397 5.0 22.5 1.0
C6 A:SOR397 5.0 20.0 1.0

Cobalt binding site 2 out of 8 in 2xin

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Cobalt binding site 2 out of 8 in the Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 2 of Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Co396

b:20.3
occ:1.00
 OE2 A:GLU217 1.9 21.2 1.0
O A:HOH602 2.1 14.0 1.0
O2 A:SOR397 2.2 13.7 1.0
NE2 A:HIS220 2.2 12.2 1.0
O A:HOH537 2.2 17.6 1.0
O1 A:SOR397 2.2 13.7 1.0
CD A:GLU217 2.8 19.6 1.0
C1 A:SOR397 3.0 13.3 1.0
C2 A:SOR397 3.1 15.0 1.0
CE1 A:HIS220 3.1 12.2 1.0
OE1 A:GLU217 3.1 20.6 1.0
CD2 A:HIS220 3.2 12.6 1.0
CO A:CO395 3.6 17.6 1.0
OD1 A:ASP257 3.7 17.7 1.0
O A:HOH588 3.8 26.7 1.0
OD2 A:ASP292 4.0 11.9 1.0
CG A:GLU217 4.2 17.4 1.0
ND1 A:HIS220 4.2 13.4 1.0
OD1 A:ASP255 4.2 32.5 1.0
CG A:HIS220 4.3 13.0 1.0
OE2 A:GLU181 4.3 14.6 1.0
OD2 A:ASP255 4.3 28.9 1.0
C3 A:SOR397 4.4 15.7 1.0
O3 A:SOR397 4.5 15.4 1.0
ND2 A:ASN247 4.5 10.1 1.0
NZ A:LYS183 4.5 9.1 1.0
CG A:ASP292 4.6 12.2 1.0
CG A:ASP257 4.6 15.1 1.0
OD2 A:ASP257 4.7 17.1 1.0
CG A:ASP255 4.7 26.9 1.0
CE A:LYS183 4.7 5.9 1.0
OD1 A:ASP292 4.7 12.3 1.0
CB A:GLU217 4.8 16.2 1.0
CD A:LYS183 4.9 7.7 1.0

Cobalt binding site 3 out of 8 in 2xin

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Cobalt binding site 3 out of 8 in the Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 3 of Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Co395

b:19.8
occ:1.00
 OE1 B:GLU217 2.1 16.0 1.0
OD2 B:ASP292 2.1 18.1 1.0
O4 B:SOR397 2.2 21.2 1.0
OE2 B:GLU181 2.3 15.3 1.0
OD2 B:ASP245 2.3 16.8 1.0
O2 B:SOR397 2.3 18.8 1.0
CD B:GLU181 3.1 16.5 1.0
CD B:GLU217 3.2 13.6 1.0
CG B:ASP292 3.2 17.4 1.0
OE1 B:GLU181 3.2 16.5 1.0
C2 B:SOR397 3.3 18.4 1.0
C4 B:SOR397 3.3 21.6 1.0
CG B:ASP245 3.3 15.2 1.0
C3 B:SOR397 3.5 19.1 1.0
CO B:CO396 3.6 19.9 1.0
O3 B:SOR397 3.6 20.7 1.0
OE2 B:GLU217 3.7 14.7 1.0
CB B:ASP292 3.8 16.4 1.0
O B:HOH459 3.8 22.9 1.0
CB B:ASP245 3.8 14.0 1.0
O B:HOH543 4.1 20.0 1.0
OD1 B:ASP292 4.2 18.5 1.0
CG B:GLU217 4.2 12.6 1.0
CE1 B:HIS220 4.3 12.2 1.0
OD1 B:ASP245 4.3 15.1 1.0
CB B:GLU217 4.3 10.6 1.0
CG B:GLU181 4.4 14.6 1.0
NE2 B:HIS220 4.5 12.2 1.0
C1 B:SOR397 4.6 17.7 1.0
C5 B:SOR397 4.6 21.9 1.0
ND2 B:ASN215 4.7 15.9 1.0
O1 B:SOR397 4.7 16.2 1.0
O6 B:SOR397 5.0 25.0 1.0

Cobalt binding site 4 out of 8 in 2xin

Go back to Cobalt Binding Sites List in 2xin
Cobalt binding site 4 out of 8 in the Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 4 of Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Co396

b:19.9
occ:1.00
 O2 B:SOR397 2.1 18.8 1.0
OE2 B:GLU217 2.1 14.7 1.0
O B:HOH543 2.2 20.0 1.0
O1 B:SOR397 2.2 16.2 1.0
O B:HOH605 2.2 14.9 1.0
NE2 B:HIS220 2.3 12.2 1.0
CD B:GLU217 2.9 13.6 1.0
CE1 B:HIS220 3.1 12.2 1.0
C1 B:SOR397 3.2 17.7 1.0
C2 B:SOR397 3.2 18.4 1.0
OE1 B:GLU217 3.3 16.0 1.0
CD2 B:HIS220 3.3 12.5 1.0
CO B:CO395 3.6 19.8 1.0
OD1 B:ASP257 3.8 18.2 1.0
O B:HOH592 3.9 25.3 1.0
OD1 B:ASP255 4.0 32.5 1.0
OD2 B:ASP292 4.1 18.1 1.0
ND1 B:HIS220 4.3 12.3 1.0
CG B:GLU217 4.3 12.6 1.0
OE2 B:GLU181 4.3 15.3 1.0
NZ B:LYS183 4.4 11.8 1.0
C3 B:SOR397 4.4 19.1 1.0
CG B:HIS220 4.4 12.5 1.0
O3 B:SOR397 4.4 20.7 1.0
ND2 B:ASN247 4.5 10.7 1.0
CG B:ASP292 4.6 17.4 1.0
CE B:LYS183 4.7 9.2 1.0
OD2 B:ASP257 4.7 19.7 1.0
CG B:ASP257 4.8 16.6 1.0
CB B:GLU217 4.8 10.6 1.0
CD B:LYS183 4.9 11.0 1.0
CG B:ASP255 4.9 28.7 1.0
OD1 B:ASP292 5.0 18.5 1.0

Cobalt binding site 5 out of 8 in 2xin

Go back to Cobalt Binding Sites List in 2xin
Cobalt binding site 5 out of 8 in the Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 5 of Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Co395

b:17.0
occ:1.00
 OD2 C:ASP245 2.0 19.8 1.0
OD2 C:ASP292 2.1 14.0 1.0
OE2 C:GLU181 2.1 13.4 1.0
OE1 C:GLU217 2.2 14.0 1.0
O4 C:SOR397 2.2 17.0 1.0
O2 C:SOR397 2.2 19.1 1.0
CD C:GLU181 3.1 14.6 1.0
CG C:ASP245 3.2 16.8 1.0
C4 C:SOR397 3.2 18.3 1.0
CG C:ASP292 3.2 12.8 1.0
C2 C:SOR397 3.2 19.0 1.0
CD C:GLU217 3.2 13.0 1.0
C3 C:SOR397 3.4 18.2 1.0
OE1 C:GLU181 3.5 16.1 1.0
CO C:CO396 3.6 18.7 1.0
O3 C:SOR397 3.7 19.4 1.0
CB C:ASP292 3.7 12.3 1.0
OE2 C:GLU217 3.8 14.2 1.0
CB C:ASP245 3.8 14.5 1.0
OD1 C:ASP245 4.1 17.8 1.0
CE1 C:HIS220 4.1 10.7 1.0
O C:HOH465 4.1 16.0 1.0
O C:HOH544 4.1 22.0 1.0
OD1 C:ASP292 4.2 11.2 1.0
NE2 C:HIS220 4.3 9.8 1.0
CG C:GLU217 4.3 10.5 1.0
CB C:GLU217 4.4 10.6 1.0
CG C:GLU181 4.4 12.0 1.0
C1 C:SOR397 4.5 17.9 1.0
C5 C:SOR397 4.5 17.8 1.0
ND2 C:ASN215 4.6 10.7 1.0
O1 C:SOR397 4.8 18.3 1.0
O6 C:SOR397 4.8 22.4 1.0
C6 C:SOR397 4.9 20.1 1.0

Cobalt binding site 6 out of 8 in 2xin

Go back to Cobalt Binding Sites List in 2xin
Cobalt binding site 6 out of 8 in the Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 6 of Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Co396

b:18.7
occ:1.00
 O2 C:SOR397 2.0 19.1 1.0
OE2 C:GLU217 2.1 14.2 1.0
NE2 C:HIS220 2.2 9.8 1.0
O C:HOH616 2.2 8.8 1.0
O1 C:SOR397 2.4 18.3 1.0
O C:HOH544 2.4 22.0 1.0
CD C:GLU217 2.9 13.0 1.0
C1 C:SOR397 3.0 17.9 1.0
C2 C:SOR397 3.0 19.0 1.0
CE1 C:HIS220 3.1 10.7 1.0
CD2 C:HIS220 3.2 10.7 1.0
OE1 C:GLU217 3.3 14.0 1.0
CO C:CO395 3.6 17.0 1.0
OD1 C:ASP257 3.7 19.0 1.0
O C:HOH615 3.9 33.2 1.0
OD2 C:ASP292 4.1 14.0 1.0
ND1 C:HIS220 4.2 12.1 1.0
CG C:HIS220 4.3 10.8 1.0
C3 C:SOR397 4.3 18.2 1.0
CG C:GLU217 4.3 10.5 1.0
OD1 C:ASP255 4.3 31.6 1.0
ND2 C:ASN247 4.4 10.5 1.0
OE2 C:GLU181 4.5 13.4 1.0
O3 C:SOR397 4.5 19.4 1.0
CG C:ASP292 4.5 12.8 1.0
OD2 C:ASP255 4.6 31.1 1.0
NZ C:LYS183 4.6 10.7 1.0
OD2 C:ASP257 4.6 19.0 1.0
CG C:ASP257 4.6 17.2 1.0
CB C:GLU217 4.8 10.6 1.0
CE C:LYS183 4.8 9.7 1.0
OD1 C:ASP292 4.8 11.2 1.0
CG C:ASP255 4.9 27.9 1.0
CD C:LYS183 5.0 11.0 1.0

Cobalt binding site 7 out of 8 in 2xin

Go back to Cobalt Binding Sites List in 2xin
Cobalt binding site 7 out of 8 in the Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 7 of Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Co395

b:18.6
occ:1.00
 OE1 D:GLU217 2.0 15.2 1.0
OD2 D:ASP292 2.0 12.5 1.0
O4 D:SOR397 2.1 17.2 1.0
OE2 D:GLU181 2.2 23.4 1.0
OD2 D:ASP245 2.2 18.3 1.0
O2 D:SOR397 2.4 16.4 1.0
CG D:ASP292 3.1 12.4 1.0
CD D:GLU181 3.1 21.4 1.0
CD D:GLU217 3.1 14.9 1.0
C2 D:SOR397 3.2 15.5 1.0
CG D:ASP245 3.3 17.5 1.0
C4 D:SOR397 3.3 17.5 1.0
OE1 D:GLU181 3.3 21.5 1.0
O3 D:SOR397 3.4 15.8 1.0
C3 D:SOR397 3.4 16.0 1.0
CO D:CO396 3.7 23.4 1.0
CB D:ASP292 3.7 11.5 1.0
CB D:ASP245 3.8 16.4 1.0
OE2 D:GLU217 3.8 17.1 1.0
O D:HOH475 3.9 21.4 1.0
O D:HOH553 4.0 19.7 1.0
OD1 D:ASP292 4.1 10.2 1.0
CG D:GLU217 4.2 12.6 1.0
CE1 D:HIS220 4.2 9.3 1.0
OD1 D:ASP245 4.3 19.5 1.0
CB D:GLU217 4.4 12.6 1.0
CG D:GLU181 4.4 17.9 1.0
NE2 D:HIS220 4.5 10.3 1.0
C5 D:SOR397 4.5 17.7 1.0
C1 D:SOR397 4.6 15.5 1.0
ND2 D:ASN215 4.6 13.3 1.0
O1 D:SOR397 4.8 13.9 1.0
O6 D:SOR397 5.0 21.2 1.0

Cobalt binding site 8 out of 8 in 2xin

Go back to Cobalt Binding Sites List in 2xin
Cobalt binding site 8 out of 8 in the Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 8 of Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Co396

b:23.4
occ:1.00
 O2 D:SOR397 1.9 16.4 1.0
O D:HOH553 2.2 19.7 1.0
NE2 D:HIS220 2.2 10.3 1.0
OE2 D:GLU217 2.2 17.1 1.0
O1 D:SOR397 2.3 13.9 1.0
O D:HOH602 2.3 77.0 1.0
CD D:GLU217 2.9 14.9 1.0
CE1 D:HIS220 3.1 9.3 1.0
C2 D:SOR397 3.1 15.5 1.0
OE1 D:GLU217 3.2 15.2 1.0
C1 D:SOR397 3.2 15.5 1.0
CD2 D:HIS220 3.3 9.7 1.0
OD1 D:ASP257 3.6 19.7 1.0
CO D:CO395 3.7 18.6 1.0
OD1 D:ASP255 3.8 32.8 1.0
O D:HOH615 3.9 23.9 1.0
OD2 D:ASP292 4.1 12.5 1.0
ND1 D:HIS220 4.2 9.7 1.0
CG D:GLU217 4.2 12.6 1.0
CG D:HIS220 4.3 10.1 1.0
C3 D:SOR397 4.4 16.0 1.0
OE2 D:GLU181 4.4 23.4 1.0
O3 D:SOR397 4.4 15.8 1.0
ND2 D:ASN247 4.4 6.8 1.0
NZ D:LYS183 4.4 9.6 1.0
CG D:ASP292 4.6 12.4 1.0
CG D:ASP257 4.6 16.2 1.0
OD2 D:ASP257 4.7 16.8 1.0
CB D:GLU217 4.8 12.6 1.0
CG D:ASP255 4.8 28.7 1.0
CE D:LYS183 4.9 8.2 1.0
OD1 D:ASP292 4.9 10.2 1.0

Reference:

J.Jenkins, J.Janin, F.Rey, M.Chiadmi, H.Van Tilbeurgh, I.Lasters, M.De Maeyer, D.Van Belle, S.J.Wodak, M.Lauwereys, P.Stanssens, G.Matthyssens, A.M.Lambeir. Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site-Directed Mutagenesis of Metal Binding Sites. Biochemistry V. 31 5449 1992.
ISSN: ISSN 0006-2960
PubMed: 1610791
DOI: 10.1021/BI00139A005
Page generated: Tue Jul 30 15:34:28 2024

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