Cobalt in PDB 3b91: Minimally Hinged Hairpin Ribozyme Incorporates ADE38(2AP) and 2',5'- Phosphodiester Linkage Mutations at the Active Site

The structure of Minimally Hinged Hairpin Ribozyme Incorporates ADE38(2AP) and 2',5'- Phosphodiester Linkage Mutations at the Active Site, PDB code: 3b91 was solved by C.Macelrevey, J.Krucinska, J.E.Wedekind, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	31.04 / 2.75
Space group	P 61 2 2
Cell size a, b, c (Å), α, β, γ (°)	94.030, 94.030, 134.290, 90.00, 90.00, 120.00
R / Rfree (%)	21.6 / 24.3

The binding sites of Cobalt atom in the Minimally Hinged Hairpin Ribozyme Incorporates ADE38(2AP) and 2',5'- Phosphodiester Linkage Mutations at the Active Site (pdb code 3b91). This binding sites where shown within 5.0 Angstroms radius around Cobalt atom.
In total 2 binding sites of Cobalt where determined in the Minimally Hinged Hairpin Ribozyme Incorporates ADE38(2AP) and 2',5'- Phosphodiester Linkage Mutations at the Active Site, PDB code: 3b91:
Jump to Cobalt binding site number: 1; 2;

Cobalt binding site 1 out of 2 in the Minimally Hinged Hairpin Ribozyme Incorporates ADE38(2AP) and 2',5'- Phosphodiester Linkage Mutations at the Active Site


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 1 of Minimally Hinged Hairpin Ribozyme Incorporates ADE38(2AP) and 2',5'- Phosphodiester Linkage Mutations at the Active Site within 5.0Å range: probe atom residue distance (Å) B Occ B:Co102 b:0.1 occ:1.00 CO B:NCO102 0.0 0.1 1.0 N5 B:NCO102 2.0 0.4 1.0 N4 B:NCO102 2.0 0.0 1.0 N3 B:NCO102 2.0 0.4 1.0 N2 B:NCO102 2.0 0.0 1.0 N1 B:NCO102 2.0 0.2 1.0 N6 B:NCO102 2.0 0.7 1.0 O6 B:G12 4.4 76.4 1.0 O2 A:SO4103 4.4 0.7 1.0 N7 B:G12 4.5 73.5 1.0 O6 B:G13 4.5 78.1 1.0 N7 B:G11 5.0 75.7 1.0

Cobalt binding site 2 out of 2 in the Minimally Hinged Hairpin Ribozyme Incorporates ADE38(2AP) and 2',5'- Phosphodiester Linkage Mutations at the Active Site


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 2 of Minimally Hinged Hairpin Ribozyme Incorporates ADE38(2AP) and 2',5'- Phosphodiester Linkage Mutations at the Active Site within 5.0Å range: probe atom residue distance (Å) B Occ C:Co101 b:1.0 occ:1.00 CO C:NCO101 0.0 1.0 1.0 N5 C:NCO101 1.9 0.5 1.0 N4 C:NCO101 2.0 0.1 1.0 N3 C:NCO101 2.0 0.1 1.0 N2 C:NCO101 2.0 0.2 1.0 N1 C:NCO101 2.0 0.1 1.0 N6 C:NCO101 2.0 0.8 1.0 OP2 C:U41 4.0 84.1 1.0 OP2 B:A20 4.0 0.4 1.0 OP1 C:A40 4.0 93.5 1.0 O6 B:G21 4.1 91.6 1.0 N7 B:G21 4.3 91.5 1.0 OP2 C:A40 4.6 89.2 1.0 P C:A40 4.8 95.9 1.0 C6 B:G21 4.9 89.1 1.0 C5 B:G21 5.0 90.0 1.0

C.Macelrevey, J.D.Salter, J.Krucinska, J.E.Wedekind. Structural Effects of Nucleobase Variations at Key Active Site Residue ADE38 in the Hairpin Ribozyme. Rna V. 14 1600 2008.
ISSN: ISSN 1355-8382
PubMed: 18596253
DOI: 10.1261/RNA.1055308