Cobalt in PDB 3fo4: Crystal Structure of Guanine Riboswitch C74U Mutant Bound to 6-Chloroguanine

The structure of Crystal Structure of Guanine Riboswitch C74U Mutant Bound to 6-Chloroguanine, PDB code: 3fo4 was solved by S.D.Gilbert, R.T.Batey, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.97 / 1.90
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	132.600, 35.170, 41.790, 90.00, 90.38, 90.00
R / Rfree (%)	22.6 / 24.9

The structure of Crystal Structure of Guanine Riboswitch C74U Mutant Bound to 6-Chloroguanine also contains other interesting chemical elements:

Chlorine

(Cl)

1 atom

Pages:

>>> Page 1 <<< Page 2, Binding sites: 11 - 12;

Binding sites:

The binding sites of Cobalt atom in the Crystal Structure of Guanine Riboswitch C74U Mutant Bound to 6-Chloroguanine (pdb code 3fo4). This binding sites where shown within 5.0 Angstroms radius around Cobalt atom.
In total 12 binding sites of Cobalt where determined in the Crystal Structure of Guanine Riboswitch C74U Mutant Bound to 6-Chloroguanine, PDB code: 3fo4:
Jump to Cobalt binding site number: 1; 2; 3; 4; 5; 6; 7; 8; 9; 10;

Cobalt binding site 1 out of 12 in the Crystal Structure of Guanine Riboswitch C74U Mutant Bound to 6-Chloroguanine


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 1 of Crystal Structure of Guanine Riboswitch C74U Mutant Bound to 6-Chloroguanine within 5.0Å range: probe atom residue distance (Å) B Occ A:Co101 b:21.8 occ:1.00 CO A:NCO101 0.0 21.8 1.0 N1 A:NCO101 2.0 21.2 1.0 N2 A:NCO101 2.0 22.4 1.0 N3 A:NCO101 2.0 21.1 1.0 N4 A:NCO101 2.0 22.4 1.0 N6 A:NCO101 2.0 21.4 1.0 N5 A:NCO101 2.0 18.4 1.0 O A:HOH339 3.8 27.6 1.0 O A:HOH405 3.8 21.1 1.0 O A:HOH342 3.9 11.8 1.0 OP1 A:U67 4.1 18.1 1.0 O A:HOH439 4.2 24.4 1.0 O A:HOH440 4.3 39.6 1.0 O3' A:A66 4.4 12.8 1.0 P A:U67 4.9 10.7 1.0 O A:HOH365 5.0 71.7 1.0

Cobalt binding site 2 out of 12 in the Crystal Structure of Guanine Riboswitch C74U Mutant Bound to 6-Chloroguanine


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 2 of Crystal Structure of Guanine Riboswitch C74U Mutant Bound to 6-Chloroguanine within 5.0Å range: probe atom residue distance (Å) B Occ A:Co102 b:33.1 occ:1.00 CO A:NCO102 0.0 33.1 1.0 N3 A:NCO102 2.0 31.8 1.0 N4 A:NCO102 2.0 32.9 1.0 N2 A:NCO102 2.0 33.2 1.0 N1 A:NCO102 2.0 30.6 1.0 N6 A:NCO102 2.0 32.5 1.0 N5 A:NCO102 2.0 30.6 1.0 O A:HOH462 3.7 30.0 1.0 O A:HOH450 3.9 25.3 1.0 OP1 A:G56 3.9 23.1 1.0 OP1 A:G38 4.0 14.2 1.0 O A:HOH456 4.1 43.5 1.0 O A:HOH359 4.2 42.6 1.0 P A:G56 4.9 24.0 1.0 OP2 A:G56 5.0 25.3 1.0

Cobalt binding site 3 out of 12 in the Crystal Structure of Guanine Riboswitch C74U Mutant Bound to 6-Chloroguanine


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 3 of Crystal Structure of Guanine Riboswitch C74U Mutant Bound to 6-Chloroguanine within 5.0Å range: probe atom residue distance (Å) B Occ A:Co103 b:27.7 occ:1.00 CO A:NCO103 0.0 27.7 1.0 N1 A:NCO103 2.0 28.4 1.0 N2 A:NCO103 2.0 29.5 1.0 N3 A:NCO103 2.0 28.3 1.0 N4 A:NCO103 2.0 28.7 1.0 N6 A:NCO103 2.0 30.1 1.0 N5 A:NCO103 2.0 28.3 1.0 O A:HOH493 3.8 41.1 1.0 O A:HOH466 3.9 18.8 1.0 O4 A:U69 4.0 14.7 1.0 O6 A:G56 4.0 21.4 1.0 O A:HOH316 4.2 11.0 1.0 OP2 A:C39 4.2 27.8 1.0 O A:HOH400 4.3 27.3 1.0 O A:HOH458 4.5 37.3 1.0 N7 A:G56 4.5 17.0 1.0 O6 A:G68 4.7 14.4 1.0 C6 A:G56 4.9 20.4 1.0 OP1 A:C39 5.0 26.8 1.0

Cobalt binding site 4 out of 12 in the Crystal Structure of Guanine Riboswitch C74U Mutant Bound to 6-Chloroguanine


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 4 of Crystal Structure of Guanine Riboswitch C74U Mutant Bound to 6-Chloroguanine within 5.0Å range: probe atom residue distance (Å) B Occ A:Co104 b:18.2 occ:1.00 CO A:NCO104 0.0 18.2 1.0 N5 A:NCO104 2.0 19.1 1.0 N6 A:NCO104 2.0 20.3 1.0 N2 A:NCO104 2.0 15.8 1.0 N3 A:NCO104 2.0 19.5 1.0 N1 A:NCO104 2.0 17.5 1.0 N4 A:NCO104 2.0 19.6 1.0 OP2 A:A66 3.8 19.3 1.0 O A:HOH308 4.0 19.7 1.0 O6 A:G32 4.0 12.0 1.0 O A:HOH319 4.0 20.1 1.0 O6 A:G31 4.1 12.6 1.0 O A:HOH307 4.1 18.5 1.0 O A:HOH343 4.2 13.5 1.0 O A:HOH352 4.3 16.0 1.0 O A:HOH341 4.3 20.6 1.0 N7 A:G31 4.4 12.9 1.0 O A:HOH305 4.8 13.6 1.0 C6 A:G31 4.9 14.8 1.0 O4 A:U30 5.0 16.8 1.0

Cobalt binding site 5 out of 12 in the Crystal Structure of Guanine Riboswitch C74U Mutant Bound to 6-Chloroguanine


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 5 of Crystal Structure of Guanine Riboswitch C74U Mutant Bound to 6-Chloroguanine within 5.0Å range: probe atom residue distance (Å) B Occ A:Co105 b:52.9 occ:1.00 CO A:NCO105 0.0 52.9 1.0 N4 A:NCO105 2.0 52.1 1.0 N1 A:NCO105 2.0 51.4 1.0 N5 A:NCO105 2.0 51.5 1.0 N6 A:NCO105 2.0 51.3 1.0 N2 A:NCO105 2.0 51.7 1.0 N3 A:NCO105 2.0 51.6 1.0 O A:HOH383 3.6 30.5 1.0 O A:HOH467 3.7 44.3 1.0 O6 A:G38 4.1 14.0 1.0 OP2 A:G37 4.1 15.4 1.0 O A:HOH419 4.1 28.5 1.0 O A:HOH338 4.2 24.2 1.0 N7 A:G38 4.4 15.6 1.0 N7 A:G37 4.5 14.9 1.0 O A:HOH453 4.5 72.1 1.0 O A:HOH349 4.7 23.6 1.0 C6 A:G38 4.9 16.4 1.0

Cobalt binding site 6 out of 12 in the Crystal Structure of Guanine Riboswitch C74U Mutant Bound to 6-Chloroguanine


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 6 of Crystal Structure of Guanine Riboswitch C74U Mutant Bound to 6-Chloroguanine within 5.0Å range: probe atom residue distance (Å) B Occ A:Co106 b:70.8 occ:1.00 CO A:NCO106 0.0 70.8 1.0 N5 A:NCO106 2.0 69.0 1.0 N6 A:NCO106 2.0 68.7 1.0 N1 A:NCO106 2.0 69.1 1.0 N3 A:NCO106 2.0 69.0 1.0 N4 A:NCO106 2.0 68.8 1.0 N2 A:NCO106 2.0 69.2 1.0 O A:HOH372 4.1 39.5 1.0 O A:HOH365 4.1 71.7 1.0 OP1 A:C26 4.8 28.8 1.0 O A:HOH318 4.9 12.0 1.0

Cobalt binding site 7 out of 12 in the Crystal Structure of Guanine Riboswitch C74U Mutant Bound to 6-Chloroguanine


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 7 of Crystal Structure of Guanine Riboswitch C74U Mutant Bound to 6-Chloroguanine within 5.0Å range: probe atom residue distance (Å) B Occ A:Co107 b:77.9 occ:1.00 CO A:NCO107 0.0 77.9 1.0 N4 A:NCO107 2.0 77.6 1.0 N5 A:NCO107 2.0 77.7 1.0 N1 A:NCO107 2.0 77.6 1.0 N2 A:NCO107 2.0 77.7 1.0 N6 A:NCO107 2.0 77.7 1.0 N3 A:NCO107 2.0 77.7 1.0 OP2 A:C41 3.8 20.8 1.0 OP1 A:C54 3.9 34.0 1.0 O A:HOH324 4.0 24.7 1.0 O A:HOH304 4.3 8.2 1.0 O A:HOH387 4.6 25.4 1.0 OP1 A:C53 4.6 29.9 1.0 OP2 A:G42 4.6 21.6 1.0 P A:C41 4.9 18.0 1.0 C5' A:C54 5.0 30.2 1.0

Cobalt binding site 8 out of 12 in the Crystal Structure of Guanine Riboswitch C74U Mutant Bound to 6-Chloroguanine


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 8 of Crystal Structure of Guanine Riboswitch C74U Mutant Bound to 6-Chloroguanine within 5.0Å range: probe atom residue distance (Å) B Occ A:Co108 b:51.9 occ:1.00 CO A:NCO108 0.0 51.9 1.0 N1 A:NCO108 2.0 51.5 1.0 N5 A:NCO108 2.0 51.5 1.0 N4 A:NCO108 2.0 51.8 1.0 N3 A:NCO108 2.0 51.3 1.0 N6 A:NCO108 2.0 51.2 1.0 N2 A:NCO108 2.0 51.5 1.0 O4 A:U47 3.7 28.8 1.0 O A:HOH435 3.9 38.0 1.0 OP2 A:U51 4.0 34.4 1.0 O A:HOH470 4.0 34.5 1.0 O A:HOH486 4.1 38.3 1.0 OP2 A:C50 4.4 37.7 1.0 OP1 A:C50 4.7 37.5 1.0 C4 A:U47 4.7 28.6 1.0 O6 A:G46 4.8 18.7 1.0 P A:C50 4.9 37.1 1.0

Cobalt binding site 9 out of 12 in the Crystal Structure of Guanine Riboswitch C74U Mutant Bound to 6-Chloroguanine


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 9 of Crystal Structure of Guanine Riboswitch C74U Mutant Bound to 6-Chloroguanine within 5.0Å range: probe atom residue distance (Å) B Occ A:Co109 b:48.7 occ:1.00 CO A:NCO109 0.0 48.7 1.0 N5 A:NCO109 2.0 47.8 1.0 N3 A:NCO109 2.0 48.0 1.0 N2 A:NCO109 2.0 47.9 1.0 N6 A:NCO109 2.0 48.1 1.0 N4 A:NCO109 2.0 47.7 1.0 N1 A:NCO109 2.0 47.7 1.0 OP1 A:A24 4.0 25.6 1.0 O A:HOH441 4.1 30.0 1.0 OP2 A:C71 4.2 16.6 1.0 O A:HOH413 4.4 28.7 1.0 O A:HOH315 4.9 21.7 1.0

Cobalt binding site 10 out of 12 in the Crystal Structure of Guanine Riboswitch C74U Mutant Bound to 6-Chloroguanine


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 10 of Crystal Structure of Guanine Riboswitch C74U Mutant Bound to 6-Chloroguanine within 5.0Å range: probe atom residue distance (Å) B Occ A:Co110 b:0.8 occ:1.00 CO A:NCO110 0.0 0.8 1.0 N5 A:NCO110 2.0 0.3 1.0 N2 A:NCO110 2.0 0.2 1.0 N4 A:NCO110 2.0 0.4 1.0 N3 A:NCO110 2.0 0.2 1.0 N1 A:NCO110 2.0 0.3 1.0 N6 A:NCO110 2.0 0.3 1.0 OP1 A:A21 3.7 31.7 1.0 OP2 A:U22 4.4 23.0 1.0 P A:A21 4.9 32.4 1.0

S.D.Gilbert, F.E.Reyes, A.L.Edwards, R.T.Batey. Adaptive Ligand Binding By the Purine Riboswitch in the Recognition of Guanine and Adenine Analogs. Structure V. 17 857 2009.
ISSN: ISSN 0969-2126
PubMed: 19523903
DOI: 10.1016/J.STR.2009.04.009