Cobalt in PDB 3got: Guanine Riboswitch C74U Mutant Bound to 2-Fluoroadenine.

The structure of Guanine Riboswitch C74U Mutant Bound to 2-Fluoroadenine., PDB code: 3got was solved by S.D.Gilbert, F.E.Reyes, R.T.Batey, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.98 / 1.95
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	132.455, 35.084, 41.891, 90.00, 90.10, 90.00
R / Rfree (%)	21.8 / 26.8

The structure of Guanine Riboswitch C74U Mutant Bound to 2-Fluoroadenine. also contains other interesting chemical elements:

Fluorine

(F)

1 atom

Pages:

>>> Page 1 <<< Page 2, Binding sites: 11 - 11;

Binding sites:

The binding sites of Cobalt atom in the Guanine Riboswitch C74U Mutant Bound to 2-Fluoroadenine. (pdb code 3got). This binding sites where shown within 5.0 Angstroms radius around Cobalt atom.
In total 11 binding sites of Cobalt where determined in the Guanine Riboswitch C74U Mutant Bound to 2-Fluoroadenine., PDB code: 3got:
Jump to Cobalt binding site number: 1; 2; 3; 4; 5; 6; 7; 8; 9; 10;

Cobalt binding site 1 out of 11 in the Guanine Riboswitch C74U Mutant Bound to 2-Fluoroadenine.


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 1 of Guanine Riboswitch C74U Mutant Bound to 2-Fluoroadenine. within 5.0Å range: probe atom residue distance (Å) B Occ A:Co101 b:28.6 occ:1.00 CO A:NCO101 0.0 28.6 1.0 N1 A:NCO101 1.9 23.4 1.0 N2 A:NCO101 1.9 26.9 1.0 N3 A:NCO101 2.0 27.2 1.0 N6 A:NCO101 2.0 25.0 1.0 N5 A:NCO101 2.0 26.5 1.0 N4 A:NCO101 2.0 28.2 1.0 O A:HOH328 3.6 36.0 1.0 O A:HOH488 3.9 40.4 1.0 O A:HOH332 4.1 29.0 1.0 OP1 A:U67 4.1 23.0 1.0 O3' A:A66 4.4 21.2 1.0 P A:U67 5.0 20.9 1.0

Cobalt binding site 2 out of 11 in the Guanine Riboswitch C74U Mutant Bound to 2-Fluoroadenine.


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 2 of Guanine Riboswitch C74U Mutant Bound to 2-Fluoroadenine. within 5.0Å range: probe atom residue distance (Å) B Occ A:Co102 b:44.8 occ:1.00 CO A:NCO102 0.0 44.8 1.0 N3 A:NCO102 2.0 43.2 1.0 N2 A:NCO102 2.0 45.4 1.0 N4 A:NCO102 2.0 43.4 1.0 N6 A:NCO102 2.0 44.6 1.0 N5 A:NCO102 2.0 44.1 1.0 N1 A:NCO102 2.0 42.9 1.0 O A:HOH451 3.8 38.4 1.0 O A:HOH310 3.9 35.4 1.0 O A:HOH396 3.9 37.6 1.0 OP1 A:G56 3.9 39.8 1.0 OP1 A:G38 4.0 20.2 1.0 O A:HOH441 4.1 35.5 1.0 O A:HOH431 4.1 31.2 1.0 O A:HOH469 4.8 40.4 1.0 C5' A:C39 4.9 31.0 1.0 OP2 A:G56 5.0 37.7 1.0 P A:G56 5.0 37.0 1.0

Cobalt binding site 3 out of 11 in the Guanine Riboswitch C74U Mutant Bound to 2-Fluoroadenine.


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 3 of Guanine Riboswitch C74U Mutant Bound to 2-Fluoroadenine. within 5.0Å range: probe atom residue distance (Å) B Occ A:Co103 b:36.0 occ:1.00 CO A:NCO103 0.0 36.0 1.0 N1 A:NCO103 2.0 33.1 1.0 N2 A:NCO103 2.0 34.4 1.0 N4 A:NCO103 2.0 37.4 1.0 N3 A:NCO103 2.0 31.8 1.0 N6 A:NCO103 2.0 36.9 1.0 N5 A:NCO103 2.0 36.1 1.0 O A:HOH474 3.6 44.8 1.0 O4 A:U69 3.8 26.7 1.0 O A:HOH446 3.8 47.1 1.0 O6 A:G56 3.9 28.1 1.0 O A:HOH390 4.0 37.9 1.0 O A:HOH309 4.1 28.2 1.0 O A:HOH442 4.3 36.8 1.0 O A:HOH500 4.3 53.5 1.0 N7 A:G56 4.4 25.9 1.0 OP2 A:C39 4.4 23.7 1.0 C6 A:G56 4.7 29.3 1.0 OP1 A:C39 4.8 24.5 1.0 O6 A:G68 4.8 24.6 1.0 C5 A:G56 4.9 28.4 1.0

Cobalt binding site 4 out of 11 in the Guanine Riboswitch C74U Mutant Bound to 2-Fluoroadenine.


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 4 of Guanine Riboswitch C74U Mutant Bound to 2-Fluoroadenine. within 5.0Å range: probe atom residue distance (Å) B Occ A:Co104 b:31.0 occ:1.00 CO A:NCO104 0.0 31.0 1.0 N6 A:NCO104 2.0 27.0 1.0 N1 A:NCO104 2.0 29.1 1.0 N2 A:NCO104 2.0 28.0 1.0 N5 A:NCO104 2.0 27.4 1.0 N3 A:NCO104 2.0 28.1 1.0 N4 A:NCO104 2.0 24.2 1.0 O A:HOH306 3.8 30.7 1.0 OP2 A:A66 3.8 33.3 1.0 O6 A:G32 3.8 24.5 1.0 O A:HOH313 4.1 29.4 1.0 O6 A:G31 4.2 22.2 1.0 O A:HOH408 4.3 26.9 1.0 O A:HOH303 4.3 23.0 1.0 O A:HOH322 4.4 31.5 1.0 N7 A:G31 4.4 24.5 1.0 O A:HOH307 4.8 28.5 1.0 C6 A:G32 4.9 25.7 1.0 O4 A:U30 4.9 22.3 1.0 C6 A:G31 5.0 24.3 1.0

Cobalt binding site 5 out of 11 in the Guanine Riboswitch C74U Mutant Bound to 2-Fluoroadenine.


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 5 of Guanine Riboswitch C74U Mutant Bound to 2-Fluoroadenine. within 5.0Å range: probe atom residue distance (Å) B Occ A:Co105 b:66.5 occ:1.00 CO A:NCO105 0.0 66.5 1.0 N5 A:NCO105 2.0 65.3 1.0 N4 A:NCO105 2.0 66.2 1.0 N2 A:NCO105 2.0 65.6 1.0 N6 A:NCO105 2.0 65.4 1.0 N1 A:NCO105 2.0 65.6 1.0 N3 A:NCO105 2.0 65.4 1.0 O A:HOH459 3.5 42.6 1.0 O6 A:G38 4.1 22.9 1.0 OP2 A:G37 4.2 25.7 1.0 O A:HOH312 4.2 31.2 1.0 N7 A:G38 4.4 23.5 1.0 O A:HOH416 4.4 46.1 1.0 O A:HOH370 4.4 48.3 1.0 O A:HOH450 4.5 34.0 1.0 N7 A:G37 4.6 26.2 1.0 O A:HOH440 4.8 33.5 1.0 C6 A:G38 5.0 25.8 1.0

Cobalt binding site 6 out of 11 in the Guanine Riboswitch C74U Mutant Bound to 2-Fluoroadenine.


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 6 of Guanine Riboswitch C74U Mutant Bound to 2-Fluoroadenine. within 5.0Å range: probe atom residue distance (Å) B Occ A:Co106 b:0.9 occ:1.00 CO A:NCO106 0.0 0.9 1.0 N5 A:NCO106 2.0 0.5 1.0 N6 A:NCO106 2.0 0.2 1.0 N3 A:NCO106 2.0 0.3 1.0 N1 A:NCO106 2.0 0.4 1.0 N2 A:NCO106 2.0 0.3 1.0 N4 A:NCO106 2.0 0.3 1.0 O A:HOH342 3.8 33.5 1.0 O A:HOH373 3.9 42.9 1.0 O A:HOH389 3.9 50.9 1.0 O A:HOH326 4.1 25.4 1.0 O A:HOH321 4.3 39.8 1.0 OP1 A:C26 4.4 44.2 1.0 O A:HOH485 4.9 56.8 1.0 O A:HOH462 4.9 46.8 1.0

Cobalt binding site 7 out of 11 in the Guanine Riboswitch C74U Mutant Bound to 2-Fluoroadenine.


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 7 of Guanine Riboswitch C74U Mutant Bound to 2-Fluoroadenine. within 5.0Å range: probe atom residue distance (Å) B Occ A:Co107 b:82.8 occ:1.00 CO A:NCO107 0.0 82.8 1.0 N1 A:NCO107 2.0 81.7 1.0 N4 A:NCO107 2.0 81.5 1.0 N6 A:NCO107 2.0 81.5 1.0 N5 A:NCO107 2.0 81.9 1.0 N2 A:NCO107 2.0 81.6 1.0 N3 A:NCO107 2.0 81.8 1.0 O A:HOH351 3.7 53.7 1.0 OP2 A:C41 3.8 26.9 1.0 OP1 A:C54 3.9 36.6 1.0 O A:HOH366 4.3 33.7 1.0 O A:HOH314 4.4 44.1 1.0 O A:HOH325 4.4 28.4 1.0 OP2 A:G42 4.6 30.0 1.0 OP1 A:C53 4.8 37.4 1.0 P A:C41 4.9 25.2 1.0 C5' A:C54 5.0 33.9 1.0 OP2 A:C53 5.0 34.7 1.0

Cobalt binding site 8 out of 11 in the Guanine Riboswitch C74U Mutant Bound to 2-Fluoroadenine.


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 8 of Guanine Riboswitch C74U Mutant Bound to 2-Fluoroadenine. within 5.0Å range: probe atom residue distance (Å) B Occ A:Co108 b:67.6 occ:1.00 CO A:NCO108 0.0 67.6 1.0 N3 A:NCO108 2.0 67.4 1.0 N6 A:NCO108 2.0 67.6 1.0 N1 A:NCO108 2.0 67.5 1.0 N2 A:NCO108 2.0 67.9 1.0 N4 A:NCO108 2.0 67.5 1.0 N5 A:NCO108 2.0 67.0 1.0 O4 A:U47 3.7 40.8 1.0 O A:HOH445 4.2 44.9 1.0 OP2 A:U51 4.3 47.8 1.0 OP2 A:C50 4.7 47.4 1.0 C4 A:U47 4.7 40.0 1.0 O6 A:G46 4.7 31.4 1.0 OP1 A:C50 4.8 48.4 1.0

Cobalt binding site 9 out of 11 in the Guanine Riboswitch C74U Mutant Bound to 2-Fluoroadenine.


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 9 of Guanine Riboswitch C74U Mutant Bound to 2-Fluoroadenine. within 5.0Å range: probe atom residue distance (Å) B Occ A:Co109 b:67.7 occ:1.00 CO A:NCO109 0.0 67.7 1.0 N1 A:NCO109 2.0 66.5 1.0 N3 A:NCO109 2.0 66.9 1.0 N2 A:NCO109 2.0 66.6 1.0 N4 A:NCO109 2.0 67.3 1.0 N5 A:NCO109 2.0 67.1 1.0 N6 A:NCO109 2.0 66.6 1.0 O A:HOH315 3.7 47.5 1.0 O A:HOH357 3.9 43.7 1.0 OP2 A:C71 4.0 26.6 1.0 OP1 A:A24 4.1 32.4 1.0 O A:HOH400 4.8 27.6 1.0 O A:HOH419 4.9 71.2 1.0

Cobalt binding site 10 out of 11 in the Guanine Riboswitch C74U Mutant Bound to 2-Fluoroadenine.


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 10 of Guanine Riboswitch C74U Mutant Bound to 2-Fluoroadenine. within 5.0Å range: probe atom residue distance (Å) B Occ A:Co111 b:1.0 occ:1.00 CO A:NCO111 0.0 1.0 1.0 N5 A:NCO111 2.0 0.7 1.0 N2 A:NCO111 2.0 0.8 1.0 N4 A:NCO111 2.0 0.6 1.0 N6 A:NCO111 2.0 0.8 1.0 N1 A:NCO111 2.0 0.7 1.0 N3 A:NCO111 2.0 0.5 1.0 O A:HOH387 3.8 64.5 1.0 O A:HOH429 3.9 29.5 1.0 OP2 A:A33 4.1 30.0 1.0 O A:HOH502 4.1 47.9 1.0 OP2 A:G32 4.2 27.9 1.0 O A:HOH496 4.4 45.9 1.0 O A:HOH329 4.7 37.8 1.0 O A:HOH364 4.8 44.8 1.0 O A:HOH417 4.9 51.3 1.0

S.D.Gilbert, F.E.Reyes, A.L.Edwards, R.T.Batey. Adaptive Ligand Binding By the Purine Riboswitch in the Recognition of Guanine and Adenine Analogs Structure V. 17 857 2009.
ISSN: ISSN 0969-2126
PubMed: 19523903
DOI: 10.1016/J.STR.2009.04.009