Cobalt in PDB 3iuh: CO2+-Bound Form of Pseudomonas Stutzeri L-Rhamnose Isomerase
Enzymatic activity of CO2+-Bound Form of Pseudomonas Stutzeri L-Rhamnose Isomerase
All present enzymatic activity of CO2+-Bound Form of Pseudomonas Stutzeri L-Rhamnose Isomerase:
5.3.1.14;
Protein crystallography data
The structure of CO2+-Bound Form of Pseudomonas Stutzeri L-Rhamnose Isomerase, PDB code: 3iuh
was solved by
H.Yoshida,
M.Yamaji,
T.Ishii,
K.Izumori,
S.Kamitori,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
39.02 /
2.00
|
Space group
|
P 1 21 1
|
Cell size a, b, c (Å), α, β, γ (°)
|
78.072,
104.960,
113.369,
90.00,
108.23,
90.00
|
R / Rfree (%)
|
18.3 /
22.5
|
Cobalt Binding Sites:
The binding sites of Cobalt atom in the CO2+-Bound Form of Pseudomonas Stutzeri L-Rhamnose Isomerase
(pdb code 3iuh). This binding sites where shown within
5.0 Angstroms radius around Cobalt atom.
In total 8 binding sites of Cobalt where determined in the
CO2+-Bound Form of Pseudomonas Stutzeri L-Rhamnose Isomerase, PDB code: 3iuh:
Jump to Cobalt binding site number:
1;
2;
3;
4;
5;
6;
7;
8;
Cobalt binding site 1 out
of 8 in 3iuh
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Cobalt Binding Sites List in 3iuh
Cobalt binding site 1 out
of 8 in the CO2+-Bound Form of Pseudomonas Stutzeri L-Rhamnose Isomerase
Mono view
Stereo pair view
|
A full contact list of Cobalt with other atoms in the Co binding
site number 1 of CO2+-Bound Form of Pseudomonas Stutzeri L-Rhamnose Isomerase within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Co501
b:49.5
occ:1.00
|
OE2
|
A:GLU219
|
2.5
|
20.1
|
1.0
|
ND1
|
A:HIS281
|
2.5
|
25.0
|
1.0
|
OD2
|
A:ASP327
|
2.5
|
22.4
|
1.0
|
OD2
|
A:ASP254
|
2.5
|
20.7
|
1.0
|
CD
|
A:GLU219
|
3.1
|
21.3
|
1.0
|
OE1
|
A:GLU219
|
3.1
|
21.9
|
1.0
|
O
|
A:HOH694
|
3.3
|
35.7
|
1.0
|
CG
|
A:ASP327
|
3.4
|
22.0
|
1.0
|
CG
|
A:HIS281
|
3.4
|
23.7
|
1.0
|
CE1
|
A:HIS281
|
3.5
|
25.8
|
1.0
|
CG
|
A:ASP254
|
3.5
|
18.7
|
1.0
|
CB
|
A:HIS281
|
3.5
|
19.6
|
1.0
|
CB
|
A:ASP327
|
3.6
|
16.7
|
1.0
|
CB
|
A:ASP254
|
3.8
|
15.1
|
1.0
|
O
|
A:HOH1319
|
3.9
|
18.1
|
1.0
|
CE1
|
A:HIS257
|
4.1
|
16.3
|
1.0
|
CG
|
A:GLU219
|
4.5
|
19.1
|
1.0
|
OD1
|
A:ASP327
|
4.5
|
23.7
|
1.0
|
CD2
|
A:HIS281
|
4.6
|
23.7
|
1.0
|
NE2
|
A:HIS281
|
4.6
|
25.1
|
1.0
|
NE2
|
A:HIS257
|
4.6
|
14.6
|
1.0
|
CO
|
A:CO502
|
4.7
|
34.6
|
0.5
|
OD1
|
A:ASP254
|
4.7
|
18.1
|
1.0
|
ND1
|
A:HIS257
|
4.9
|
14.8
|
1.0
|
CE
|
A:MET325
|
4.9
|
23.8
|
1.0
|
CA
|
A:ASP254
|
5.0
|
15.1
|
1.0
|
|
Cobalt binding site 2 out
of 8 in 3iuh
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Cobalt Binding Sites List in 3iuh
Cobalt binding site 2 out
of 8 in the CO2+-Bound Form of Pseudomonas Stutzeri L-Rhamnose Isomerase
Mono view
Stereo pair view
|
A full contact list of Cobalt with other atoms in the Co binding
site number 2 of CO2+-Bound Form of Pseudomonas Stutzeri L-Rhamnose Isomerase within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Co502
b:34.6
occ:0.50
|
O
|
A:HOH1319
|
2.0
|
18.1
|
1.0
|
OD2
|
A:ASP289
|
2.2
|
22.3
|
1.0
|
NE2
|
A:HIS257
|
2.2
|
14.6
|
1.0
|
O
|
A:HOH711
|
2.7
|
28.2
|
1.0
|
CG
|
A:ASP289
|
2.9
|
24.9
|
1.0
|
OD1
|
A:ASP289
|
3.0
|
25.9
|
1.0
|
CE1
|
A:HIS257
|
3.1
|
16.3
|
1.0
|
CD2
|
A:HIS257
|
3.2
|
14.9
|
1.0
|
OD1
|
A:ASP291
|
3.3
|
20.5
|
1.0
|
OD2
|
A:ASP254
|
3.7
|
20.7
|
1.0
|
OD2
|
A:ASP291
|
3.9
|
21.3
|
1.0
|
CG
|
A:ASP291
|
4.0
|
19.7
|
1.0
|
NZ
|
A:LYS221
|
4.2
|
20.0
|
1.0
|
ND1
|
A:HIS257
|
4.2
|
14.8
|
1.0
|
CG
|
A:ASP254
|
4.3
|
18.7
|
1.0
|
CB
|
A:ASP289
|
4.3
|
20.4
|
1.0
|
CG
|
A:HIS257
|
4.3
|
16.7
|
1.0
|
CE
|
A:LYS221
|
4.4
|
19.2
|
1.0
|
OD1
|
A:ASP254
|
4.5
|
18.1
|
1.0
|
NH2
|
B:ARG65
|
4.6
|
11.6
|
1.0
|
CO
|
A:CO501
|
4.7
|
49.5
|
1.0
|
OD2
|
A:ASP327
|
4.7
|
22.4
|
1.0
|
CD
|
A:LYS221
|
4.8
|
17.5
|
1.0
|
OE2
|
A:GLU219
|
4.9
|
20.1
|
1.0
|
|
Cobalt binding site 3 out
of 8 in 3iuh
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Cobalt Binding Sites List in 3iuh
Cobalt binding site 3 out
of 8 in the CO2+-Bound Form of Pseudomonas Stutzeri L-Rhamnose Isomerase
Mono view
Stereo pair view
|
A full contact list of Cobalt with other atoms in the Co binding
site number 3 of CO2+-Bound Form of Pseudomonas Stutzeri L-Rhamnose Isomerase within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Co503
b:53.2
occ:1.00
|
OD2
|
B:ASP327
|
2.5
|
27.1
|
1.0
|
ND1
|
B:HIS281
|
2.5
|
17.8
|
1.0
|
OD2
|
B:ASP254
|
2.6
|
18.1
|
1.0
|
O
|
B:HOH1274
|
2.9
|
33.1
|
1.0
|
OE2
|
B:GLU219
|
3.0
|
27.5
|
1.0
|
OE1
|
B:GLU219
|
3.0
|
27.2
|
1.0
|
CG
|
B:ASP327
|
3.2
|
23.1
|
1.0
|
CD
|
B:GLU219
|
3.3
|
25.2
|
1.0
|
CB
|
B:ASP327
|
3.4
|
20.1
|
1.0
|
CG
|
B:HIS281
|
3.5
|
20.4
|
1.0
|
CE1
|
B:HIS281
|
3.5
|
19.9
|
1.0
|
CB
|
B:HIS281
|
3.6
|
18.9
|
1.0
|
CG
|
B:ASP254
|
3.6
|
18.4
|
1.0
|
O
|
B:HOH760
|
3.8
|
23.1
|
1.0
|
CB
|
B:ASP254
|
3.9
|
16.8
|
1.0
|
OD1
|
B:ASP327
|
4.3
|
24.5
|
1.0
|
CE1
|
B:HIS257
|
4.5
|
14.8
|
1.0
|
CG
|
B:GLU219
|
4.6
|
20.9
|
1.0
|
CD2
|
B:HIS281
|
4.6
|
19.5
|
1.0
|
NE2
|
B:HIS281
|
4.6
|
18.8
|
1.0
|
CE
|
B:MET325
|
4.7
|
25.4
|
1.0
|
OD1
|
B:ASP254
|
4.7
|
18.8
|
1.0
|
NE2
|
B:HIS257
|
4.8
|
17.8
|
1.0
|
CO
|
B:CO504
|
4.9
|
36.7
|
0.5
|
CA
|
B:ASP327
|
5.0
|
18.3
|
1.0
|
|
Cobalt binding site 4 out
of 8 in 3iuh
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Cobalt Binding Sites List in 3iuh
Cobalt binding site 4 out
of 8 in the CO2+-Bound Form of Pseudomonas Stutzeri L-Rhamnose Isomerase
Mono view
Stereo pair view
|
A full contact list of Cobalt with other atoms in the Co binding
site number 4 of CO2+-Bound Form of Pseudomonas Stutzeri L-Rhamnose Isomerase within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Co504
b:36.7
occ:0.50
|
NE2
|
B:HIS257
|
2.2
|
17.8
|
1.0
|
OD2
|
B:ASP289
|
2.2
|
31.7
|
1.0
|
O
|
B:HOH760
|
2.4
|
23.1
|
1.0
|
OD1
|
B:ASP289
|
2.7
|
34.8
|
1.0
|
CG
|
B:ASP289
|
2.8
|
31.9
|
1.0
|
O
|
B:HOH532
|
2.9
|
26.1
|
1.0
|
CE1
|
B:HIS257
|
3.1
|
14.8
|
1.0
|
OD1
|
B:ASP291
|
3.2
|
20.9
|
1.0
|
CD2
|
B:HIS257
|
3.2
|
16.4
|
1.0
|
OD2
|
B:ASP291
|
3.7
|
21.6
|
1.0
|
CG
|
B:ASP291
|
3.9
|
22.4
|
1.0
|
NZ
|
B:LYS221
|
4.0
|
16.0
|
1.0
|
OD2
|
B:ASP254
|
4.0
|
18.1
|
1.0
|
CB
|
B:ASP289
|
4.2
|
27.7
|
1.0
|
ND1
|
B:HIS257
|
4.3
|
16.4
|
1.0
|
CG
|
B:HIS257
|
4.3
|
17.3
|
1.0
|
CE
|
B:LYS221
|
4.3
|
15.9
|
1.0
|
OD1
|
B:ASP254
|
4.4
|
18.8
|
1.0
|
CG
|
B:ASP254
|
4.4
|
18.4
|
1.0
|
NH2
|
A:ARG65
|
4.5
|
24.6
|
1.0
|
CD
|
B:LYS221
|
4.6
|
16.8
|
1.0
|
O
|
B:HOH991
|
4.9
|
36.5
|
1.0
|
CO
|
B:CO503
|
4.9
|
53.2
|
1.0
|
OE2
|
B:GLU219
|
5.0
|
27.5
|
1.0
|
|
Cobalt binding site 5 out
of 8 in 3iuh
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Cobalt Binding Sites List in 3iuh
Cobalt binding site 5 out
of 8 in the CO2+-Bound Form of Pseudomonas Stutzeri L-Rhamnose Isomerase
Mono view
Stereo pair view
|
A full contact list of Cobalt with other atoms in the Co binding
site number 5 of CO2+-Bound Form of Pseudomonas Stutzeri L-Rhamnose Isomerase within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:Co505
b:50.8
occ:1.00
|
ND1
|
C:HIS281
|
2.5
|
16.3
|
1.0
|
OE2
|
C:GLU219
|
2.5
|
21.4
|
1.0
|
OD2
|
C:ASP254
|
2.5
|
14.9
|
1.0
|
OD2
|
C:ASP327
|
2.6
|
19.7
|
1.0
|
OE1
|
C:GLU219
|
3.0
|
18.8
|
1.0
|
CD
|
C:GLU219
|
3.0
|
19.3
|
1.0
|
O
|
C:HOH1169
|
3.3
|
31.0
|
1.0
|
CG
|
C:HIS281
|
3.4
|
15.7
|
1.0
|
CE1
|
C:HIS281
|
3.5
|
18.1
|
1.0
|
CG
|
C:ASP254
|
3.5
|
14.1
|
1.0
|
CG
|
C:ASP327
|
3.5
|
17.2
|
1.0
|
CB
|
C:HIS281
|
3.6
|
15.8
|
1.0
|
CB
|
C:ASP327
|
3.7
|
13.7
|
1.0
|
CB
|
C:ASP254
|
3.8
|
13.5
|
1.0
|
O
|
C:HOH1153
|
4.2
|
24.5
|
1.0
|
CE1
|
C:HIS257
|
4.3
|
13.6
|
1.0
|
CG
|
C:GLU219
|
4.4
|
17.8
|
1.0
|
CD2
|
C:HIS281
|
4.6
|
16.0
|
1.0
|
NE2
|
C:HIS281
|
4.6
|
15.5
|
1.0
|
OD1
|
C:ASP254
|
4.7
|
14.2
|
1.0
|
OD1
|
C:ASP327
|
4.7
|
14.1
|
1.0
|
NE2
|
C:HIS257
|
4.7
|
13.7
|
1.0
|
CE
|
C:MET325
|
4.8
|
17.6
|
1.0
|
CA
|
C:ASP254
|
4.9
|
13.6
|
1.0
|
ND1
|
C:HIS257
|
5.0
|
16.4
|
1.0
|
|
Cobalt binding site 6 out
of 8 in 3iuh
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Cobalt Binding Sites List in 3iuh
Cobalt binding site 6 out
of 8 in the CO2+-Bound Form of Pseudomonas Stutzeri L-Rhamnose Isomerase
Mono view
Stereo pair view
|
A full contact list of Cobalt with other atoms in the Co binding
site number 6 of CO2+-Bound Form of Pseudomonas Stutzeri L-Rhamnose Isomerase within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:Co506
b:46.1
occ:0.50
|
OD2
|
C:ASP289
|
2.1
|
28.1
|
1.0
|
O
|
C:HOH1153
|
2.1
|
24.5
|
1.0
|
NE2
|
C:HIS257
|
2.4
|
13.7
|
1.0
|
O
|
C:HOH570
|
2.5
|
19.4
|
1.0
|
CG
|
C:ASP289
|
2.8
|
27.9
|
1.0
|
OD1
|
C:ASP289
|
2.9
|
31.2
|
1.0
|
O
|
C:HOH1169
|
3.0
|
31.0
|
1.0
|
OD1
|
C:ASP291
|
3.1
|
19.6
|
1.0
|
CE1
|
C:HIS257
|
3.3
|
13.6
|
1.0
|
CD2
|
C:HIS257
|
3.4
|
13.6
|
1.0
|
OD2
|
C:ASP291
|
3.5
|
20.1
|
1.0
|
CG
|
C:ASP291
|
3.7
|
21.0
|
1.0
|
NZ
|
C:LYS221
|
3.8
|
12.2
|
1.0
|
CE
|
C:LYS221
|
4.1
|
12.3
|
1.0
|
CB
|
C:ASP289
|
4.3
|
22.5
|
1.0
|
NH2
|
D:ARG65
|
4.3
|
14.0
|
1.0
|
OD2
|
C:ASP254
|
4.3
|
14.9
|
1.0
|
ND1
|
C:HIS257
|
4.4
|
16.4
|
1.0
|
CG
|
C:HIS257
|
4.5
|
15.2
|
1.0
|
CD
|
C:LYS221
|
4.6
|
15.2
|
1.0
|
OD1
|
C:ASP254
|
4.6
|
14.2
|
1.0
|
O
|
C:HOH973
|
4.6
|
31.3
|
1.0
|
CG
|
C:ASP254
|
4.6
|
14.1
|
1.0
|
CZ
|
D:PHE66
|
4.8
|
17.0
|
1.0
|
OD2
|
C:ASP327
|
5.0
|
19.7
|
1.0
|
|
Cobalt binding site 7 out
of 8 in 3iuh
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Cobalt Binding Sites List in 3iuh
Cobalt binding site 7 out
of 8 in the CO2+-Bound Form of Pseudomonas Stutzeri L-Rhamnose Isomerase
Mono view
Stereo pair view
|
A full contact list of Cobalt with other atoms in the Co binding
site number 7 of CO2+-Bound Form of Pseudomonas Stutzeri L-Rhamnose Isomerase within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
D:Co507
b:45.1
occ:1.00
|
OE2
|
D:GLU219
|
2.3
|
20.3
|
1.0
|
OD2
|
D:ASP254
|
2.5
|
13.1
|
1.0
|
OD2
|
D:ASP327
|
2.5
|
16.2
|
1.0
|
ND1
|
D:HIS281
|
2.6
|
20.5
|
1.0
|
CD
|
D:GLU219
|
3.1
|
19.1
|
1.0
|
O
|
D:HOH994
|
3.1
|
37.4
|
1.0
|
OE1
|
D:GLU219
|
3.2
|
19.9
|
1.0
|
CG
|
D:ASP327
|
3.3
|
15.9
|
1.0
|
CG
|
D:ASP254
|
3.5
|
13.2
|
1.0
|
CG
|
D:HIS281
|
3.6
|
20.2
|
1.0
|
O
|
D:HOH1320
|
3.6
|
18.1
|
1.0
|
CE1
|
D:HIS281
|
3.6
|
21.6
|
1.0
|
CB
|
D:ASP327
|
3.6
|
13.9
|
1.0
|
CB
|
D:HIS281
|
3.7
|
17.8
|
1.0
|
CB
|
D:ASP254
|
3.8
|
11.7
|
1.0
|
CE1
|
D:HIS257
|
4.2
|
6.6
|
1.0
|
CG
|
D:GLU219
|
4.4
|
15.3
|
1.0
|
OD1
|
D:ASP327
|
4.4
|
16.6
|
1.0
|
NE2
|
D:HIS257
|
4.5
|
7.0
|
1.0
|
OD1
|
D:ASP254
|
4.6
|
10.2
|
1.0
|
CO
|
D:CO508
|
4.7
|
45.2
|
0.5
|
NE2
|
D:HIS281
|
4.7
|
22.1
|
1.0
|
CD2
|
D:HIS281
|
4.7
|
20.8
|
1.0
|
CE
|
D:MET325
|
4.9
|
14.0
|
1.0
|
ND1
|
D:HIS257
|
5.0
|
7.1
|
1.0
|
|
Cobalt binding site 8 out
of 8 in 3iuh
Go back to
Cobalt Binding Sites List in 3iuh
Cobalt binding site 8 out
of 8 in the CO2+-Bound Form of Pseudomonas Stutzeri L-Rhamnose Isomerase
Mono view
Stereo pair view
|
A full contact list of Cobalt with other atoms in the Co binding
site number 8 of CO2+-Bound Form of Pseudomonas Stutzeri L-Rhamnose Isomerase within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
D:Co508
b:45.2
occ:0.50
|
O
|
D:HOH1320
|
2.1
|
18.1
|
1.0
|
NE2
|
D:HIS257
|
2.2
|
7.0
|
1.0
|
OD2
|
D:ASP289
|
2.3
|
29.1
|
1.0
|
O
|
D:HOH497
|
2.6
|
15.5
|
1.0
|
OD1
|
D:ASP289
|
2.8
|
29.8
|
1.0
|
CG
|
D:ASP289
|
2.8
|
25.9
|
1.0
|
CE1
|
D:HIS257
|
3.1
|
6.6
|
1.0
|
CD2
|
D:HIS257
|
3.1
|
7.8
|
1.0
|
OD1
|
D:ASP291
|
3.2
|
16.7
|
1.0
|
OD2
|
D:ASP291
|
3.8
|
17.1
|
1.0
|
CG
|
D:ASP291
|
3.9
|
16.2
|
1.0
|
OD2
|
D:ASP254
|
3.9
|
13.1
|
1.0
|
NZ
|
D:LYS221
|
4.0
|
8.2
|
1.0
|
CE
|
D:LYS221
|
4.2
|
6.7
|
1.0
|
ND1
|
D:HIS257
|
4.2
|
7.1
|
1.0
|
CB
|
D:ASP289
|
4.3
|
22.1
|
1.0
|
CG
|
D:HIS257
|
4.3
|
8.7
|
1.0
|
CG
|
D:ASP254
|
4.3
|
13.2
|
1.0
|
OD1
|
D:ASP254
|
4.4
|
10.2
|
1.0
|
NH2
|
C:ARG65
|
4.5
|
13.2
|
1.0
|
CO
|
D:CO507
|
4.7
|
45.1
|
1.0
|
CD
|
D:LYS221
|
4.7
|
8.8
|
1.0
|
OE2
|
D:GLU219
|
4.9
|
20.3
|
1.0
|
OD2
|
D:ASP327
|
5.0
|
16.2
|
1.0
|
|
Reference:
H.Yoshida,
M.Yamaji,
T.Ishii,
K.Izumori,
S.Kamitori.
Catalytic Reaction Mechanism of Pseudomonas Stutzeri L-Rhamnose Isomerase Deduced From X-Ray Structures Febs J. V. 277 1045 2010.
ISSN: ISSN 1742-464X
PubMed: 20088877
DOI: 10.1111/J.1742-4658.2009.07548.X
Page generated: Tue Jul 30 16:12:09 2024
|