Cobalt in the structure of Crystal Structure of Rnase T In Complex With A Non-Preferred Ssdna (Gc) With One Mg in the Active Site (pdb 3ngz)

The binding sites of Cobalt atom in the structure of Crystal Structure of Rnase T In Complex With A Non-Preferred Ssdna (Gc) With One Mg in the Active Site (pdb code 3ngz). This binding sites where shown with 5.0 Angstroms radius around Cobalt atom. The 3ngz structure was solved by Y.-Y.HSIAO, H.S.YUAN, with X-Ray Crystallography technique, brief refinement statistics is given in the table below: Resolution (A) 28.5-2.1 Space group P21212 a (A) 96.747 b (A) 105.585 c (A) 47.180 alpha (°) 90.00 beta (°) 90.00 gamma (°) 90.00 Rfactor (%) 19.5 Rfree (%) 26 Cobalt Binding Sites: Cobalt binding site 1 out of 2 in 3ngz Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 1 of Cobalt in the PDB 3ngz. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Cobalt atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: A: Leu36, A: Val62, A: Glu63, A: Phe65, A: Gly92, A: Ala132, A: Arg135, A: Hoh227, A: Hoh243, A: Hoh352, A: Hoh353, conact list: Atom Atom Distance (A) Co CB A:Leu36 4.35 Co CD2 A:Leu36 4.59 Co CG1 A:Val62 4.29 Co O A:Glu63 4.64 Co N A:Phe65 4.65 Co CB A:Phe65 3.93 Co CD2 A:Phe65 4.11 Co CG A:Phe65 4.49 Co CA A:Phe65 4.77 Co N A:Gly92 4.30 Co C A:Gly92 4.88 Co CA A:Gly92 3.79 Co CB A:Ala132 4.27 Co CA A:Ala132 4.69 Co CD A:Arg135 4.78 Co CZ A:Arg135 3.82 Co NE A:Arg135 3.54 Co NH2 A:Arg135 3.21 Co O A:Hoh227 2.99 Co O A:Hoh243 3.14 Co O A:Hoh352 3.29 Co O A:Hoh353 4.76 interactive model: Cobalt binding site 2 out of 2 in 3ngz Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 2 of Cobalt in the PDB 3ngz. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Cobalt atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: B: Leu36, B: Val62, B: Glu63, B: Phe65, B: Gly92, B: Ala132, B: Arg135, B: Hoh223, B: Hoh233, B: Hoh248, B: Hoh331, conact list: Atom Atom Distance (A) Co CB B:Leu36 4.35 Co CD2 B:Leu36 4.70 Co CG1 B:Val62 4.34 Co O B:Glu63 4.68 Co N B:Phe65 4.72 Co CB B:Phe65 3.98 Co CD2 B:Phe65 4.12 Co CG B:Phe65 4.51 Co CA B:Phe65 4.82 Co N B:Gly92 4.36 Co CA B:Gly92 3.92 Co CB B:Ala132 4.17 Co CA B:Ala132 4.55 Co CD B:Arg135 4.79 Co CZ B:Arg135 3.83 Co NE B:Arg135 3.55 Co NH2 B:Arg135 3.21 Co O B:Hoh223 3.33 Co O B:Hoh233 3.04 Co O B:Hoh248 3.29 Co O B:Hoh331 4.73 interactive model: