Cobalt in PDB 3p0f: Structure of HUPP2 in An Inactive Conformation with Bound 5- Benzylacyclouridine

All present enzymatic activity of Structure of HUPP2 in An Inactive Conformation with Bound 5- Benzylacyclouridine:
2.4.2.3;

The structure of Structure of HUPP2 in An Inactive Conformation with Bound 5- Benzylacyclouridine, PDB code: 3p0f was solved by T.P.Roosild, S.Castronovo, A.Villoso, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 1.54
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	59.696, 59.696, 189.307, 90.00, 90.00, 90.00
R / Rfree (%)	19.1 / 21.2

The structure of Structure of HUPP2 in An Inactive Conformation with Bound 5- Benzylacyclouridine also contains other interesting chemical elements:

Magnesium

(Mg)

1 atom

The binding sites of Cobalt atom in the Structure of HUPP2 in An Inactive Conformation with Bound 5- Benzylacyclouridine (pdb code 3p0f). This binding sites where shown within 5.0 Angstroms radius around Cobalt atom.
In total 2 binding sites of Cobalt where determined in the Structure of HUPP2 in An Inactive Conformation with Bound 5- Benzylacyclouridine, PDB code: 3p0f:
Jump to Cobalt binding site number: 1; 2;

Cobalt binding site 1 out of 2 in the Structure of HUPP2 in An Inactive Conformation with Bound 5- Benzylacyclouridine


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 1 of Structure of HUPP2 in An Inactive Conformation with Bound 5- Benzylacyclouridine within 5.0Å range: probe atom residue distance (Å) B Occ A:Co1 b:17.1 occ:1.00 O A:HOH399 2.0 20.1 1.0 NE2 A:HIS212 2.1 16.2 1.0 O A:HOH471 2.2 21.1 1.0 O A:ALA248 2.2 15.2 1.0 CD2 A:HIS212 3.1 18.2 1.0 CE1 A:HIS212 3.1 17.4 1.0 C A:ALA248 3.4 13.8 1.0 CA A:GLY249 4.0 13.9 1.0 N A:GLY249 4.2 13.5 1.0 ND1 A:HIS212 4.2 17.1 1.0 CG A:HIS212 4.3 16.1 1.0 O A:HOH572 4.3 33.6 1.0 O A:HOH473 4.4 29.2 1.0 CA A:ALA248 4.5 13.6 1.0

Cobalt binding site 2 out of 2 in the Structure of HUPP2 in An Inactive Conformation with Bound 5- Benzylacyclouridine


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 2 of Structure of HUPP2 in An Inactive Conformation with Bound 5- Benzylacyclouridine within 5.0Å range: probe atom residue distance (Å) B Occ A:Co2 b:28.9 occ:1.00 NE2 A:HIS133 2.1 24.1 1.0 OD1 A:ASP33 2.1 24.1 1.0 O A:HOH383 2.1 20.3 1.0 O A:HOH488 2.2 31.1 1.0 O A:HOH437 2.2 29.7 1.0 O A:HOH521 2.2 38.5 1.0 CE1 A:HIS133 3.0 24.6 1.0 CG A:ASP33 3.0 24.8 1.0 CD2 A:HIS133 3.1 23.4 1.0 OD2 A:ASP33 3.3 27.6 1.0 O A:HOH416 4.1 25.0 1.0 O A:HOH480 4.1 31.9 1.0 ND1 A:HIS133 4.1 23.4 1.0 CG A:HIS133 4.2 19.5 1.0 O A:HOH538 4.4 32.7 1.0 CB A:ASP33 4.4 21.7 1.0 O A:HOH15 4.4 19.2 1.0 CA A:ASP33 4.7 20.9 1.0 N A:ASP33 4.8 19.5 1.0 CD2 A:LEU32 4.8 19.9 1.0 CG2 A:VAL27 4.9 23.8 1.0

T.P.Roosild, S.Castronovo, A.Villoso, A.Ziemba, G.Pizzorno. A Novel Structural Mechanism For Redox Regulation of Uridine Phosphorylase 2 Activity. J.Struct.Biol. V. 176 229 2011.
ISSN: ISSN 1047-8477
PubMed: 21855639
DOI: 10.1016/J.JSB.2011.08.002