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Cobalt in PDB 3tk2: Crystallographic Structure of Phenylalanine Hydroxylase From Chromobacterium Violaceum Cocrystallized with Phenylalanine in A Site Distal to the Active Site

Enzymatic activity of Crystallographic Structure of Phenylalanine Hydroxylase From Chromobacterium Violaceum Cocrystallized with Phenylalanine in A Site Distal to the Active Site

All present enzymatic activity of Crystallographic Structure of Phenylalanine Hydroxylase From Chromobacterium Violaceum Cocrystallized with Phenylalanine in A Site Distal to the Active Site:
1.14.16.1;

Protein crystallography data

The structure of Crystallographic Structure of Phenylalanine Hydroxylase From Chromobacterium Violaceum Cocrystallized with Phenylalanine in A Site Distal to the Active Site, PDB code: 3tk2 was solved by J.A.Ronau, M.M.Abu-Omar, C.Das, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 24.49 / 1.35
Space group P 1
Cell size a, b, c (Å), α, β, γ (°) 36.934, 38.561, 47.800, 76.56, 72.90, 85.41
R / Rfree (%) 15.9 / 20.7

Cobalt Binding Sites:

The binding sites of Cobalt atom in the Crystallographic Structure of Phenylalanine Hydroxylase From Chromobacterium Violaceum Cocrystallized with Phenylalanine in A Site Distal to the Active Site (pdb code 3tk2). This binding sites where shown within 5.0 Angstroms radius around Cobalt atom.
In total 2 binding sites of Cobalt where determined in the Crystallographic Structure of Phenylalanine Hydroxylase From Chromobacterium Violaceum Cocrystallized with Phenylalanine in A Site Distal to the Active Site, PDB code: 3tk2:
Jump to Cobalt binding site number: 1; 2;

Cobalt binding site 1 out of 2 in 3tk2

Go back to Cobalt Binding Sites List in 3tk2
Cobalt binding site 1 out of 2 in the Crystallographic Structure of Phenylalanine Hydroxylase From Chromobacterium Violaceum Cocrystallized with Phenylalanine in A Site Distal to the Active Site


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 1 of Crystallographic Structure of Phenylalanine Hydroxylase From Chromobacterium Violaceum Cocrystallized with Phenylalanine in A Site Distal to the Active Site within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Co299

b:10.5
occ:1.00
 NE2 A:HIS138 2.0 11.1 1.0
O A:HOH344 2.1 15.4 1.0
OE2 A:GLU184 2.1 10.8 1.0
O A:HOH362 2.1 14.4 1.0
NE2 A:HIS143 2.2 11.2 1.0
OE1 A:GLU184 2.3 10.6 1.0
CD A:GLU184 2.5 9.1 1.0
CE1 A:HIS138 2.9 10.9 1.0
CD2 A:HIS138 3.0 10.8 1.0
CE1 A:HIS143 3.1 9.5 1.0
CD2 A:HIS143 3.2 10.3 1.0
O A:HOH353 3.8 15.5 1.0
CG A:GLU184 4.0 7.5 1.0
ND1 A:HIS138 4.1 11.8 1.0
OD1 A:ASP139 4.1 17.3 1.0
CG A:HIS138 4.2 9.3 1.0
ND1 A:HIS143 4.3 8.9 1.0
O A:HOH308 4.3 32.8 1.0
CG A:HIS143 4.3 9.2 1.0
O A:HOH442 4.3 24.4 1.0
CB A:ALA199 4.6 8.9 1.0
O A:HOH487 4.9 31.2 1.0
CB A:GLU184 4.9 6.8 1.0

Cobalt binding site 2 out of 2 in 3tk2

Go back to Cobalt Binding Sites List in 3tk2
Cobalt binding site 2 out of 2 in the Crystallographic Structure of Phenylalanine Hydroxylase From Chromobacterium Violaceum Cocrystallized with Phenylalanine in A Site Distal to the Active Site


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 2 of Crystallographic Structure of Phenylalanine Hydroxylase From Chromobacterium Violaceum Cocrystallized with Phenylalanine in A Site Distal to the Active Site within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Co300

b:27.2
occ:1.00
 NE2 A:HIS22 2.0 13.8 1.0
O A:HOH333 2.4 17.7 1.0
O A:HOH334 2.4 34.9 1.0
CE1 A:HIS22 2.9 14.9 1.0
CD2 A:HIS22 3.1 19.2 1.0
ND1 A:HIS22 4.0 17.2 1.0
CG A:HIS22 4.2 17.2 1.0
O A:HOH516 4.9 27.9 1.0

Reference:

J.A.Ronau, L.N.Paul, J.E.Fuchs, I.R.Corn, K.T.Wagner, K.R.Liedl, M.M.Abu-Omar, C.Das. An Additional Substrate Binding Site in A Bacterial Phenylalanine Hydroxylase. Eur.Biophys.J. V. 42 691 2013.
ISSN: ISSN 0175-7571
PubMed: 23860686
DOI: 10.1007/S00249-013-0919-8
Page generated: Tue Jul 30 16:37:16 2024

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