Cobalt in PDB 3tk4: Crystal Structure of Phenylalanine Hydroxylase From Chromobacterium Violaceum Bound to Cobalt

All present enzymatic activity of Crystal Structure of Phenylalanine Hydroxylase From Chromobacterium Violaceum Bound to Cobalt:
1.14.16.1;

The structure of Crystal Structure of Phenylalanine Hydroxylase From Chromobacterium Violaceum Bound to Cobalt, PDB code: 3tk4 was solved by J.A.Ronau, M.M.Abu-Omar, C.Das, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	37.51 / 1.50
Space group	P 1
Cell size a, b, c (Å), α, β, γ (°)	36.885, 38.577, 47.877, 76.49, 73.15, 85.39
R / Rfree (%)	17.1 / 21

The binding sites of Cobalt atom in the Crystal Structure of Phenylalanine Hydroxylase From Chromobacterium Violaceum Bound to Cobalt (pdb code 3tk4). This binding sites where shown within 5.0 Angstroms radius around Cobalt atom.
In total 2 binding sites of Cobalt where determined in the Crystal Structure of Phenylalanine Hydroxylase From Chromobacterium Violaceum Bound to Cobalt, PDB code: 3tk4:
Jump to Cobalt binding site number: 1; 2;

Cobalt binding site 1 out of 2 in the Crystal Structure of Phenylalanine Hydroxylase From Chromobacterium Violaceum Bound to Cobalt


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 1 of Crystal Structure of Phenylalanine Hydroxylase From Chromobacterium Violaceum Bound to Cobalt within 5.0Å range: probe atom residue distance (Å) B Occ A:Co298 b:9.3 occ:1.00 NE2 A:HIS138 2.0 8.6 1.0 O A:HOH309 2.1 11.8 1.0 NE2 A:HIS143 2.1 10.4 1.0 OE2 A:GLU184 2.1 11.9 1.0 OE1 A:GLU184 2.2 9.2 1.0 O A:HOH472 2.2 14.8 1.0 CD A:GLU184 2.5 9.0 1.0 CE1 A:HIS138 3.0 9.4 1.0 CE1 A:HIS143 3.1 10.3 1.0 CD2 A:HIS138 3.1 8.2 1.0 CD2 A:HIS143 3.1 9.3 1.0 O A:HOH307 3.9 15.3 1.0 CG A:GLU184 4.0 7.8 1.0 ND1 A:HIS138 4.1 9.8 1.0 OD1 A:ASP139 4.1 16.7 1.0 CG A:HIS138 4.2 7.9 1.0 ND1 A:HIS143 4.2 9.9 1.0 CG A:HIS143 4.2 8.9 1.0 O A:HOH404 4.3 27.6 1.0 O A:HOH395 4.4 31.2 1.0 CB A:ALA199 4.6 8.3 1.0 CB A:GLU184 4.9 6.9 1.0

Cobalt binding site 2 out of 2 in the Crystal Structure of Phenylalanine Hydroxylase From Chromobacterium Violaceum Bound to Cobalt


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 2 of Crystal Structure of Phenylalanine Hydroxylase From Chromobacterium Violaceum Bound to Cobalt within 5.0Å range: probe atom residue distance (Å) B Occ A:Co299 b:43.6 occ:1.00 NE2 A:HIS22 2.4 24.8 1.0 CE1 A:HIS22 3.3 24.2 1.0 CD2 A:HIS22 3.5 23.0 1.0 O A:HOH474 4.3 55.7 1.0 ND1 A:HIS22 4.5 23.1 1.0 O A:HOH437 4.5 26.7 1.0 CG A:HIS22 4.6 20.8 1.0

J.A.Ronau, L.N.Paul, J.E.Fuchs, I.R.Corn, K.T.Wagner, K.R.Liedl, M.M.Abu-Omar, C.Das. An Additional Substrate Binding Site in A Bacterial Phenylalanine Hydroxylase. Eur.Biophys.J. V. 42 691 2013.
ISSN: ISSN 0175-7571
PubMed: 23860686
DOI: 10.1007/S00249-013-0919-8