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Cobalt in PDB 3v9w: Crystal Structure of Rnase T in Complex with A Preferred Ssdna (Tta) with Two Mg in the Active Site

Protein crystallography data

The structure of Crystal Structure of Rnase T in Complex with A Preferred Ssdna (Tta) with Two Mg in the Active Site, PDB code: 3v9w was solved by Y.-Y.Hsiao, H.S.Yuan, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 26.39 / 1.70
Space group P 32
Cell size a, b, c (Å), α, β, γ (°) 46.340, 46.340, 315.354, 90.00, 90.00, 120.00
R / Rfree (%) 19.2 / 22.6

Other elements in 3v9w:

The structure of Crystal Structure of Rnase T in Complex with A Preferred Ssdna (Tta) with Two Mg in the Active Site also contains other interesting chemical elements:

Magnesium (Mg) 6 atoms

Cobalt Binding Sites:

The binding sites of Cobalt atom in the Crystal Structure of Rnase T in Complex with A Preferred Ssdna (Tta) with Two Mg in the Active Site (pdb code 3v9w). This binding sites where shown within 5.0 Angstroms radius around Cobalt atom.
In total 6 binding sites of Cobalt where determined in the Crystal Structure of Rnase T in Complex with A Preferred Ssdna (Tta) with Two Mg in the Active Site, PDB code: 3v9w:
Jump to Cobalt binding site number: 1; 2; 3; 4; 5; 6;

Cobalt binding site 1 out of 6 in 3v9w

Go back to Cobalt Binding Sites List in 3v9w
Cobalt binding site 1 out of 6 in the Crystal Structure of Rnase T in Complex with A Preferred Ssdna (Tta) with Two Mg in the Active Site


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 1 of Crystal Structure of Rnase T in Complex with A Preferred Ssdna (Tta) with Two Mg in the Active Site within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Co302

b:35.7
occ:1.00
O A:HOH423 3.0 19.7 1.0
O A:HOH429 3.0 26.0 1.0
O A:HOH427 3.2 21.4 1.0
NH2 A:ARG135 3.2 28.2 1.0
NE A:ARG135 3.7 30.1 1.0
CA A:GLY92 3.8 20.8 1.0
CZ A:ARG135 3.9 27.9 1.0
CB A:PHE65 4.0 28.2 1.0
CB A:ALA132 4.2 19.6 1.0
CD2 A:PHE65 4.2 21.5 1.0
N A:GLY92 4.2 18.9 1.0
CB A:LEU36 4.3 18.4 1.0
CG1 A:VAL62 4.4 18.3 1.0
CG A:PHE65 4.5 28.2 1.0
CA A:ALA132 4.5 20.6 1.0
CD2 A:LEU36 4.6 20.6 1.0
O A:HOH488 4.6 32.9 1.0
O A:GLU63 4.7 23.6 1.0
N A:PHE65 4.7 23.4 1.0
O A:HOH477 4.7 31.3 1.0
O A:HOH483 4.8 32.5 1.0
CA A:PHE65 4.8 27.0 1.0
C A:GLY92 4.9 22.1 1.0
CD A:ARG135 5.0 28.3 1.0

Cobalt binding site 2 out of 6 in 3v9w

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Cobalt binding site 2 out of 6 in the Crystal Structure of Rnase T in Complex with A Preferred Ssdna (Tta) with Two Mg in the Active Site


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 2 of Crystal Structure of Rnase T in Complex with A Preferred Ssdna (Tta) with Two Mg in the Active Site within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Co302

b:37.3
occ:1.00
O B:HOH429 3.0 25.6 1.0
O B:HOH451 3.0 21.1 1.0
O B:HOH447 3.1 25.9 1.0
NH2 B:ARG135 3.3 24.9 1.0
NE B:ARG135 3.7 33.5 1.0
CA B:GLY92 3.7 22.6 1.0
CZ B:ARG135 3.9 29.0 1.0
CB B:PHE65 4.0 25.4 1.0
CB B:ALA132 4.1 18.4 1.0
CD2 B:PHE65 4.2 19.9 1.0
N B:GLY92 4.2 21.1 1.0
CB B:LEU36 4.3 19.2 1.0
CG1 B:VAL62 4.4 17.5 1.0
CD2 B:LEU36 4.4 16.8 1.0
CA B:ALA132 4.5 20.9 1.0
CG B:PHE65 4.5 28.3 1.0
O B:HOH437 4.7 28.7 1.0
O B:GLU63 4.7 23.8 1.0
O B:HOH448 4.7 30.7 1.0
N B:PHE65 4.8 21.2 1.0
CA B:PHE65 4.8 25.4 1.0
O B:HOH479 4.9 29.7 1.0
C B:GLY92 4.9 19.0 1.0
CD B:ARG135 4.9 31.4 1.0
CG B:LEU36 4.9 21.9 1.0

Cobalt binding site 3 out of 6 in 3v9w

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Cobalt binding site 3 out of 6 in the Crystal Structure of Rnase T in Complex with A Preferred Ssdna (Tta) with Two Mg in the Active Site


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 3 of Crystal Structure of Rnase T in Complex with A Preferred Ssdna (Tta) with Two Mg in the Active Site within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Co301

b:16.9
occ:1.00
OP1 G:DA7 2.0 12.9 1.0
OD2 C:ASP186 2.0 19.3 1.0
OE1 C:GLU25 2.1 18.5 1.0
ND1 C:HIS181 2.3 22.4 1.0
CD C:GLU25 2.9 17.5 1.0
OE2 C:GLU25 3.0 15.1 1.0
CG C:ASP186 3.0 16.9 1.0
CE1 C:HIS181 3.2 22.0 1.0
P G:DA7 3.2 11.9 1.0
CG C:HIS181 3.3 20.7 1.0
CA C:HIS181 3.5 18.1 1.0
OD1 C:ASP186 3.6 18.4 1.0
CB C:HIS181 3.7 18.6 1.0
OP2 G:DA7 3.8 13.0 1.0
OD2 C:ASP23 3.8 14.5 1.0
O C:HOH406 3.9 15.9 1.0
OD1 C:ASP23 3.9 14.8 1.0
O5' G:DA7 4.0 16.7 1.0
N C:HIS181 4.0 19.0 1.0
MG G:MG101 4.0 13.0 1.0
CB C:ASP186 4.2 13.1 1.0
O G:HOH202 4.3 16.3 1.0
CG C:ASP23 4.3 14.7 1.0
NE2 C:HIS181 4.3 23.3 1.0
CG C:GLU25 4.3 19.1 1.0
C5' G:DA7 4.4 14.1 1.0
O3' G:DT6 4.4 12.0 1.0
O C:HOH423 4.4 20.3 1.0
CD2 C:HIS181 4.4 20.8 1.0
C C:HIS181 4.6 22.9 1.0
O C:VAL24 4.7 14.9 1.0
O C:HIS181 5.0 20.9 1.0

Cobalt binding site 4 out of 6 in 3v9w

Go back to Cobalt Binding Sites List in 3v9w
Cobalt binding site 4 out of 6 in the Crystal Structure of Rnase T in Complex with A Preferred Ssdna (Tta) with Two Mg in the Active Site


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 4 of Crystal Structure of Rnase T in Complex with A Preferred Ssdna (Tta) with Two Mg in the Active Site within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Co302

b:34.6
occ:1.00
O C:HOH571 3.0 25.4 1.0
O C:HOH414 3.1 22.5 1.0
O C:HOH415 3.1 16.4 1.0
NE C:ARG135 3.3 37.6 1.0
NH2 C:ARG135 3.5 28.6 1.0
CA C:GLY92 3.7 25.0 1.0
CZ C:ARG135 3.9 25.9 1.0
CB C:ALA132 4.0 18.8 1.0
CB C:PHE65 4.1 20.4 1.0
N C:GLY92 4.1 20.9 1.0
CG1 C:VAL62 4.2 18.0 1.0
CB C:LEU36 4.2 14.4 1.0
CD2 C:PHE65 4.2 25.2 1.0
CD C:ARG135 4.4 24.8 1.0
CA C:ALA132 4.4 19.0 1.0
CD2 C:LEU36 4.4 14.0 1.0
O C:HOH475 4.6 33.1 1.0
CG C:PHE65 4.6 24.8 1.0
O C:GLU63 4.6 22.0 1.0
O C:HOH478 4.7 25.1 1.0
N C:PHE65 4.8 21.2 1.0
CG C:LEU36 4.8 14.9 1.0
O C:HOH485 4.8 27.5 1.0
C C:GLY92 4.8 24.1 1.0
CA C:PHE65 4.9 25.1 1.0
CG C:ARG135 4.9 27.8 1.0
O C:ALA132 5.0 24.4 1.0

Cobalt binding site 5 out of 6 in 3v9w

Go back to Cobalt Binding Sites List in 3v9w
Cobalt binding site 5 out of 6 in the Crystal Structure of Rnase T in Complex with A Preferred Ssdna (Tta) with Two Mg in the Active Site


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 5 of Crystal Structure of Rnase T in Complex with A Preferred Ssdna (Tta) with Two Mg in the Active Site within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Co301

b:17.5
occ:1.00
OP1 H:DA7 1.9 12.8 1.0
OD2 D:ASP186 2.0 18.7 1.0
OE1 D:GLU25 2.1 17.8 1.0
ND1 D:HIS181 2.3 22.8 1.0
CD D:GLU25 2.9 17.2 1.0
OE2 D:GLU25 3.0 14.7 1.0
CG D:ASP186 3.1 19.1 1.0
CE1 D:HIS181 3.2 21.3 1.0
P H:DA7 3.2 13.1 1.0
CG D:HIS181 3.4 18.0 1.0
CA D:HIS181 3.5 19.0 1.0
OD1 D:ASP186 3.6 19.6 1.0
CB D:HIS181 3.7 21.3 1.0
O D:HOH411 3.8 15.6 1.0
OP2 H:DA7 3.8 13.0 1.0
OD2 D:ASP23 3.9 14.4 1.0
OD1 D:ASP23 4.0 14.4 1.0
O5' H:DA7 4.0 14.5 1.0
MG H:MG101 4.0 13.1 1.0
N D:HIS181 4.0 20.9 1.0
CB D:ASP186 4.2 15.4 1.0
O H:HOH202 4.2 17.0 1.0
C5' H:DA7 4.3 11.6 1.0
CG D:ASP23 4.3 15.0 1.0
CG D:GLU25 4.3 19.2 1.0
NE2 D:HIS181 4.3 18.9 1.0
O D:HOH412 4.4 17.3 1.0
O3' H:DT6 4.4 13.8 1.0
CD2 D:HIS181 4.4 21.0 1.0
C D:HIS181 4.6 21.0 1.0
O D:VAL24 4.7 14.9 1.0
O D:HIS181 4.9 21.4 1.0

Cobalt binding site 6 out of 6 in 3v9w

Go back to Cobalt Binding Sites List in 3v9w
Cobalt binding site 6 out of 6 in the Crystal Structure of Rnase T in Complex with A Preferred Ssdna (Tta) with Two Mg in the Active Site


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 6 of Crystal Structure of Rnase T in Complex with A Preferred Ssdna (Tta) with Two Mg in the Active Site within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Co302

b:33.2
occ:1.00
O D:HOH441 3.0 23.9 1.0
O D:HOH415 3.0 16.9 1.0
O D:HOH420 3.1 22.1 1.0
NH1 D:ARG135 3.4 25.3 1.0
CA D:GLY92 3.8 25.0 1.0
CD D:ARG135 3.9 22.5 1.0
CB D:PHE65 4.0 20.2 1.0
CB D:ALA132 4.1 18.5 1.0
N D:GLY92 4.2 22.2 1.0
CD2 D:PHE65 4.2 23.2 1.0
CG1 D:VAL62 4.2 19.1 1.0
CB D:LEU36 4.2 15.2 1.0
CZ D:ARG135 4.4 17.1 1.0
CA D:ALA132 4.5 18.2 1.0
CD2 D:LEU36 4.5 17.4 1.0
CG D:PHE65 4.5 20.9 1.0
CG D:ARG135 4.6 23.4 1.0
NE D:ARG135 4.6 23.9 1.0
O D:HOH453 4.6 26.8 1.0
O D:GLU63 4.7 24.4 1.0
O D:HOH472 4.7 24.1 1.0
N D:PHE65 4.7 20.6 1.0
CA D:PHE65 4.8 24.2 1.0
O D:HOH531 4.8 30.8 1.0
CG D:LEU36 4.9 15.2 1.0
C D:GLY92 4.9 21.2 1.0
O D:LEU36 4.9 17.2 1.0
N D:LEU36 5.0 14.8 1.0

Reference:

Y.-Y.Hsiao, Y.Duh, Y.P.Chen, Y.T.Wang, H.S.Yuan. How An Exonuclease Decides Where to Stop in Trimming of Nucleic Acids: Crystal Structures of Rnase T-Product Complexes Nucleic Acids Res. V. 40 8144 2012.
ISSN: ISSN 0305-1048
PubMed: 22718982
DOI: 10.1093/NAR/GKS548
Page generated: Tue Jul 30 16:46:10 2024

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