Atomistry » Cobalt » PDB 3w72-4aq4 » 3zgj
Atomistry »
  Cobalt »
    PDB 3w72-4aq4 »
      3zgj »

Cobalt in PDB 3zgj: S221M V223F Y359A Mutant of 4-Hydroxymandelate Synthase From Streptomyces Coelicolor

Enzymatic activity of S221M V223F Y359A Mutant of 4-Hydroxymandelate Synthase From Streptomyces Coelicolor

All present enzymatic activity of S221M V223F Y359A Mutant of 4-Hydroxymandelate Synthase From Streptomyces Coelicolor:
1.13.11.46;

Protein crystallography data

The structure of S221M V223F Y359A Mutant of 4-Hydroxymandelate Synthase From Streptomyces Coelicolor, PDB code: 3zgj was solved by S.Pratter, G.Straganz, G.Grogan, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 54.95 / 1.95
Space group P 4
Cell size a, b, c (Å), α, β, γ (°) 122.870, 122.870, 54.860, 90.00, 90.00, 90.00
R / Rfree (%) 17.505 / 21.504

Cobalt Binding Sites:

The binding sites of Cobalt atom in the S221M V223F Y359A Mutant of 4-Hydroxymandelate Synthase From Streptomyces Coelicolor (pdb code 3zgj). This binding sites where shown within 5.0 Angstroms radius around Cobalt atom.
In total 2 binding sites of Cobalt where determined in the S221M V223F Y359A Mutant of 4-Hydroxymandelate Synthase From Streptomyces Coelicolor, PDB code: 3zgj:
Jump to Cobalt binding site number: 1; 2;

Cobalt binding site 1 out of 2 in 3zgj

Go back to Cobalt Binding Sites List in 3zgj
Cobalt binding site 1 out of 2 in the S221M V223F Y359A Mutant of 4-Hydroxymandelate Synthase From Streptomyces Coelicolor


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 1 of S221M V223F Y359A Mutant of 4-Hydroxymandelate Synthase From Streptomyces Coelicolor within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Co1368

b:27.1
occ:1.00
OE1 A:GLU340 2.1 22.8 1.0
O12 A:RMN1369 2.1 41.7 1.0
NE2 A:HIS181 2.1 17.6 1.0
NE2 A:HIS261 2.1 25.5 1.0
O8 A:RMN1369 2.3 34.5 1.0
C10 A:RMN1369 2.8 42.0 1.0
C7 A:RMN1369 3.0 39.6 1.0
CE1 A:HIS181 3.0 19.8 1.0
CE1 A:HIS261 3.1 25.7 1.0
CD2 A:HIS261 3.1 22.5 1.0
CD A:GLU340 3.1 24.7 1.0
CD2 A:HIS181 3.1 18.4 1.0
OE2 A:GLU340 3.5 28.4 1.0
C1 A:RMN1369 3.7 43.9 1.0
O11 A:RMN1369 3.9 54.3 1.0
C6 A:RMN1369 4.2 36.0 1.0
ND1 A:HIS181 4.2 19.5 1.0
ND1 A:HIS261 4.2 23.7 1.0
CG A:HIS181 4.3 18.4 1.0
CG A:HIS261 4.3 23.3 1.0
NE2 A:GLN325 4.3 28.0 1.0
CE1 A:PHE327 4.3 30.3 1.0
CG A:GLU340 4.4 22.7 1.0
CB A:ALA263 4.5 22.1 1.0
CZ A:PHE327 4.6 26.9 1.0
CB A:GLU340 4.6 20.6 1.0
C2 A:RMN1369 4.6 42.0 1.0
CB A:ALA183 4.7 19.6 1.0
OG1 A:THR234 4.8 28.0 1.0

Cobalt binding site 2 out of 2 in 3zgj

Go back to Cobalt Binding Sites List in 3zgj
Cobalt binding site 2 out of 2 in the S221M V223F Y359A Mutant of 4-Hydroxymandelate Synthase From Streptomyces Coelicolor


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 2 of S221M V223F Y359A Mutant of 4-Hydroxymandelate Synthase From Streptomyces Coelicolor within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Co1368

b:21.0
occ:1.00
OE1 B:GLU340 1.8 19.6 1.0
NE2 B:HIS181 2.1 16.8 1.0
NE2 B:HIS261 2.1 19.6 1.0
O12 B:RMN1369 2.1 37.8 1.0
O8 B:RMN1369 2.3 33.5 1.0
CE1 B:HIS181 2.9 17.3 1.0
CD B:GLU340 3.0 23.7 1.0
CD2 B:HIS261 3.0 20.0 1.0
C10 B:RMN1369 3.1 46.5 1.0
CE1 B:HIS261 3.1 20.9 1.0
CD2 B:HIS181 3.1 18.1 1.0
C7 B:RMN1369 3.3 46.9 1.0
OE2 B:GLU340 3.5 21.6 1.0
C1 B:RMN1369 4.0 46.8 1.0
ND1 B:HIS181 4.1 16.8 1.0
CG B:HIS261 4.2 19.2 1.0
ND1 B:HIS261 4.2 19.5 1.0
CG B:HIS181 4.2 16.4 1.0
O11 B:RMN1369 4.2 50.7 1.0
CE1 B:PHE327 4.2 20.9 1.0
CG B:GLU340 4.3 22.7 1.0
CB B:ALA263 4.4 15.7 1.0
NE2 B:GLN325 4.4 23.4 1.0
C2 B:RMN1369 4.5 44.5 1.0
CB B:GLU340 4.5 17.4 1.0
CZ B:PHE327 4.6 21.9 1.0
C6 B:RMN1369 4.7 45.6 1.0
CB B:ALA183 4.7 17.1 1.0
OG1 B:THR234 4.9 32.9 1.0

Reference:

S.Pratter, C.Konstantinovics, C.L.M.Digiuro, E.Leitner, D.Kumar, S.P.De Visser, G.Grogan, G.Straganz. Inversion of Enantioselectivity of A Mononuclear Non-Heme Iron(II)-Dependent Hydroxylase By Tuning the Interplay of Metal Center Geometry and Protein Structure Angew.Chem.Int.Ed.Engl. V. 52 9677 2013.
ISSN: ISSN 1433-7851
PubMed: 23881738
DOI: 10.1002/ANGE.201304633
Page generated: Sun Jul 13 19:36:34 2025

Last articles

Fe in 2YXO
Fe in 2YRS
Fe in 2YXC
Fe in 2YNM
Fe in 2YVJ
Fe in 2YP1
Fe in 2YU2
Fe in 2YU1
Fe in 2YQB
Fe in 2YOO
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy