Cobalt in PDB 4a8i: Protein Crystallization and Microgravity: Glucose Isomerase Crystals Grown During the Pcdf-Protein Mission

All present enzymatic activity of Protein Crystallization and Microgravity: Glucose Isomerase Crystals Grown During the Pcdf-Protein Mission:
5.3.1.5;

The structure of Protein Crystallization and Microgravity: Glucose Isomerase Crystals Grown During the Pcdf-Protein Mission, PDB code: 4a8i was solved by K.Decanniere, L.-D.Patino-Lopez, M.Sleutel, C.Evrard, C.Van De Weerdt, E.Haumont, J.A.Gavira, F.Otalora, D.Maes, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	70.87 / 0.95
Space group	I 2 2 2
Cell size a, b, c (Å), α, β, γ (°)	92.410, 98.140, 102.080, 90.00, 90.00, 90.00
R / Rfree (%)	11.951 / 13.226

The binding sites of Cobalt atom in the Protein Crystallization and Microgravity: Glucose Isomerase Crystals Grown During the Pcdf-Protein Mission (pdb code 4a8i). This binding sites where shown within 5.0 Angstroms radius around Cobalt atom.
In total 2 binding sites of Cobalt where determined in the Protein Crystallization and Microgravity: Glucose Isomerase Crystals Grown During the Pcdf-Protein Mission, PDB code: 4a8i:
Jump to Cobalt binding site number: 1; 2;

Cobalt binding site 1 out of 2 in the Protein Crystallization and Microgravity: Glucose Isomerase Crystals Grown During the Pcdf-Protein Mission


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 1 of Protein Crystallization and Microgravity: Glucose Isomerase Crystals Grown During the Pcdf-Protein Mission within 5.0Å range: probe atom residue distance (Å) B Occ A:Co1389 b:10.6 occ:1.00 OE1 A:GLU217 2.1 17.1 1.0 OD2 A:ASP287 2.2 12.1 1.0 OE2 A:GLU181 2.2 13.5 1.0 OD2 A:ASP245 2.3 11.4 1.0 O2 A:EDO1388 2.4 10.8 1.0 O A:HOH2272 2.4 16.5 1.0 CD A:GLU181 3.1 10.5 1.0 CG A:ASP287 3.2 7.7 1.0 CD A:GLU217 3.3 9.8 1.0 C2 A:EDO1388 3.3 11.6 1.0 OE1 A:GLU181 3.4 10.7 1.0 CG A:ASP245 3.4 7.8 1.0 CB A:ASP287 3.6 7.0 1.0 O A:HOH2297 4.0 19.0 1.0 CB A:ASP245 4.0 7.1 1.0 O A:HOH2327 4.0 13.4 1.0 CE1 A:HIS220 4.1 15.0 1.0 CG A:GLU217 4.1 6.6 1.0 CB A:GLU217 4.2 6.2 1.0 OE2 A:GLU217 4.3 14.5 1.0 OD1 A:ASP287 4.3 8.1 1.0 OD1 A:ASP245 4.4 9.5 1.0 CG A:GLU181 4.4 8.5 1.0 NE2 A:HIS220 4.5 14.2 1.0 C1 A:EDO1388 4.6 9.8 1.0 ND2 A:ASN215 4.8 11.8 1.0 ND1 A:HIS220 4.9 10.6 1.0 CO A:CO1390 4.9 17.5 1.0

Cobalt binding site 2 out of 2 in the Protein Crystallization and Microgravity: Glucose Isomerase Crystals Grown During the Pcdf-Protein Mission


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 2 of Protein Crystallization and Microgravity: Glucose Isomerase Crystals Grown During the Pcdf-Protein Mission within 5.0Å range: probe atom residue distance (Å) B Occ A:Co1390 b:17.5 occ:1.00 OE2 A:GLU217 2.1 14.5 1.0 O A:HOH2297 2.1 19.0 1.0 OD1 A:ASP255 2.3 15.8 1.0 OD2 A:ASP255 2.4 16.6 1.0 OD1 A:ASP257 2.4 19.0 1.0 CG A:ASP255 2.7 14.7 1.0 NE2 A:HIS220 2.8 14.2 1.0 CD A:GLU217 3.0 9.8 1.0 O A:HOH2276 3.2 47.4 1.0 CD2 A:HIS220 3.2 10.5 1.0 OE1 A:GLU217 3.4 17.1 1.0 CG A:ASP257 3.4 14.6 1.0 OD2 A:ASP257 3.6 21.3 1.0 CE1 A:HIS220 3.9 15.0 1.0 ND2 A:ASN247 4.0 7.2 1.0 CB A:ASP255 4.1 15.1 1.0 O A:HOH2329 4.1 13.3 1.0 CG A:GLU217 4.3 6.6 1.0 CG A:HIS220 4.5 7.5 1.0 O A:HOH2272 4.5 16.5 1.0 NZ A:LYS183 4.7 13.6 1.0 OD2 A:ASP287 4.7 12.1 1.0 ND1 A:HIS220 4.8 10.6 1.0 CE A:LYS183 4.8 9.0 1.0 CB A:ASP257 4.8 11.1 1.0 CO A:CO1389 4.9 10.6 1.0 CA A:ASP255 4.9 9.3 1.0

K.Decanniere, L.-D.Patino-Lopez, M.Sleutel, C.Evrard, C.Van De Weerdt, E.Haumont, J.A.Gavira, F.Otalora, D.Maes. Protein Crystallization and Microgravity: Glucose Isomerase Crystals Grown During the Pcdf-Protein Mission To Be Published.