Cobalt in PDB 4a8n: Protein Crystallization and Microgravity: Glucose Isomerase Crystals Grown During the Pcdf-Protein Mission

All present enzymatic activity of Protein Crystallization and Microgravity: Glucose Isomerase Crystals Grown During the Pcdf-Protein Mission:
5.3.1.5;

The structure of Protein Crystallization and Microgravity: Glucose Isomerase Crystals Grown During the Pcdf-Protein Mission, PDB code: 4a8n was solved by K.Decanniere, L.-D.Patino-Lopez, M.Sleutel, C.Evrard, C.Van De Weerdt, E.Haumont, J.A.Gavira, F.Otalora, D.Maes, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	70.77 / 1.20
Space group	I 2 2 2
Cell size a, b, c (Å), α, β, γ (°)	92.530, 98.070, 102.050, 90.00, 90.00, 90.00
R / Rfree (%)	10.998 / 12.788

The binding sites of Cobalt atom in the Protein Crystallization and Microgravity: Glucose Isomerase Crystals Grown During the Pcdf-Protein Mission (pdb code 4a8n). This binding sites where shown within 5.0 Angstroms radius around Cobalt atom.
In total 2 binding sites of Cobalt where determined in the Protein Crystallization and Microgravity: Glucose Isomerase Crystals Grown During the Pcdf-Protein Mission, PDB code: 4a8n:
Jump to Cobalt binding site number: 1; 2;

Cobalt binding site 1 out of 2 in the Protein Crystallization and Microgravity: Glucose Isomerase Crystals Grown During the Pcdf-Protein Mission


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 1 of Protein Crystallization and Microgravity: Glucose Isomerase Crystals Grown During the Pcdf-Protein Mission within 5.0Å range: probe atom residue distance (Å) B Occ A:Co1390 b:11.0 occ:1.00 OE1 A:GLU217 2.1 15.2 1.0 OD2 A:ASP287 2.1 11.1 1.0 OE2 A:GLU181 2.1 13.0 1.0 OD2 A:ASP245 2.3 10.2 1.0 O1 A:GOL1389 2.4 10.5 1.0 O A:HOH2258 2.4 16.2 1.0 CD A:GLU181 3.1 9.5 1.0 CG A:ASP287 3.2 6.8 1.0 CD A:GLU217 3.3 7.8 1.0 OE1 A:GLU181 3.3 10.2 1.0 C1 A:GOL1389 3.4 12.6 1.0 CG A:ASP245 3.4 6.5 1.0 CB A:ASP287 3.7 5.8 1.0 O A:HOH2277 3.9 14.9 1.0 O A:HOH2303 4.0 11.8 1.0 CB A:ASP245 4.0 5.6 1.0 CE1 A:HIS220 4.1 13.7 1.0 CG A:GLU217 4.1 5.2 1.0 CB A:GLU217 4.2 4.6 1.0 OE2 A:GLU217 4.2 11.4 1.0 OD1 A:ASP287 4.3 6.8 1.0 OD1 A:ASP245 4.4 7.8 1.0 CG A:GLU181 4.4 7.6 1.0 NE2 A:HIS220 4.5 13.0 1.0 C2 A:GOL1389 4.7 9.3 1.0 ND2 A:ASN215 4.8 10.0 1.0 ND1 A:HIS220 4.8 9.1 1.0 CO A:CO1391 4.9 14.9 1.0

Cobalt binding site 2 out of 2 in the Protein Crystallization and Microgravity: Glucose Isomerase Crystals Grown During the Pcdf-Protein Mission


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 2 of Protein Crystallization and Microgravity: Glucose Isomerase Crystals Grown During the Pcdf-Protein Mission within 5.0Å range: probe atom residue distance (Å) B Occ A:Co1391 b:14.9 occ:1.00 OE2 A:GLU217 2.0 11.4 1.0 O A:HOH2277 2.1 14.9 1.0 OD1 A:ASP255 2.2 14.0 1.0 OD1 A:ASP257 2.3 13.4 1.0 OD2 A:ASP255 2.3 15.3 1.0 NE2 A:HIS220 2.7 13.0 1.0 CG A:ASP255 2.7 12.2 1.0 CD A:GLU217 3.0 7.8 1.0 CD2 A:HIS220 3.2 8.2 1.0 CG A:ASP257 3.3 12.7 1.0 O A:HOH2261 3.4 32.6 1.0 OE1 A:GLU217 3.4 15.2 1.0 OD2 A:ASP257 3.5 18.9 1.0 CE1 A:HIS220 3.8 13.7 1.0 ND2 A:ASN247 4.0 5.9 1.0 CB A:ASP255 4.1 11.8 1.0 O A:HOH2305 4.1 10.7 1.0 CG A:GLU217 4.4 5.2 1.0 CG A:HIS220 4.4 6.0 1.0 O A:HOH2258 4.4 16.2 1.0 NZ A:LYS183 4.7 13.0 1.0 ND1 A:HIS220 4.7 9.1 1.0 CB A:ASP257 4.7 10.0 1.0 OD2 A:ASP287 4.7 11.1 1.0 CE A:LYS183 4.8 7.4 1.0 CO A:CO1390 4.9 11.0 1.0 CA A:ASP255 5.0 7.2 1.0

K.Decanniere, L.-D.Patino-Lopez, M.Sleutel, C.Evrard, C.Van De Weerdt, E.Haumont, J.A.Gavira, F.Otalora, D.Maes. Protein Crystallization and Microgravity: Glucose Isomerase Crystals Grown During the Pcdf-Protein Mission To Be Published.