Cobalt in PDB 4a8r: Protein Crystallization and Microgravity: Glucose Isomerase Crystals Grown During the Pcdf-Protein Mission

All present enzymatic activity of Protein Crystallization and Microgravity: Glucose Isomerase Crystals Grown During the Pcdf-Protein Mission:
5.3.1.5;

The structure of Protein Crystallization and Microgravity: Glucose Isomerase Crystals Grown During the Pcdf-Protein Mission, PDB code: 4a8r was solved by K.Decanniere, L.-D.Patino-Lopez, M.Sleutel, C.Evrard, C.Van De Weerdt, E.Haumont, J.A.Gavira, F.Otalora, D.Maes, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	70.77 / 1.42
Space group	I 2 2 2
Cell size a, b, c (Å), α, β, γ (°)	92.530, 98.220, 102.070, 90.00, 90.00, 90.00
R / Rfree (%)	10.291 / 12.971

The binding sites of Cobalt atom in the Protein Crystallization and Microgravity: Glucose Isomerase Crystals Grown During the Pcdf-Protein Mission (pdb code 4a8r). This binding sites where shown within 5.0 Angstroms radius around Cobalt atom.
In total 2 binding sites of Cobalt where determined in the Protein Crystallization and Microgravity: Glucose Isomerase Crystals Grown During the Pcdf-Protein Mission, PDB code: 4a8r:
Jump to Cobalt binding site number: 1; 2;

Cobalt binding site 1 out of 2 in the Protein Crystallization and Microgravity: Glucose Isomerase Crystals Grown During the Pcdf-Protein Mission


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 1 of Protein Crystallization and Microgravity: Glucose Isomerase Crystals Grown During the Pcdf-Protein Mission within 5.0Å range: probe atom residue distance (Å) B Occ A:Co1390 b:14.8 occ:1.00 OE1 A:GLU217 2.0 15.8 1.0 OD2 A:ASP287 2.0 11.2 1.0 OE2 A:GLU181 2.1 16.8 1.0 O1 A:GOL1389 2.3 12.3 1.0 OD2 A:ASP245 2.3 10.1 1.0 O A:HOH2262 2.4 25.6 1.0 CD A:GLU181 3.1 12.3 1.0 CG A:ASP287 3.2 6.6 1.0 CD A:GLU217 3.3 7.4 1.0 C1 A:GOL1389 3.3 16.4 1.0 OE1 A:GLU181 3.4 11.2 1.0 CG A:ASP245 3.5 7.6 1.0 CB A:ASP287 3.6 6.1 1.0 O A:HOH2310 3.9 15.8 1.0 O A:HOH2283 4.0 15.2 1.0 CB A:ASP245 4.0 6.3 1.0 CE1 A:HIS220 4.0 15.3 1.0 OE2 A:GLU217 4.1 12.1 1.0 CB A:GLU217 4.2 5.6 1.0 CG A:GLU217 4.2 6.2 1.0 OD1 A:ASP287 4.3 7.9 1.0 CG A:GLU181 4.4 7.5 1.0 OD1 A:ASP245 4.4 8.4 1.0 NE2 A:HIS220 4.5 11.7 1.0 C2 A:GOL1389 4.6 9.8 1.0 ND2 A:ASN215 4.9 9.8 1.0 ND1 A:HIS220 4.9 9.7 1.0 CO A:CO1391 5.0 21.1 1.0

Cobalt binding site 2 out of 2 in the Protein Crystallization and Microgravity: Glucose Isomerase Crystals Grown During the Pcdf-Protein Mission


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 2 of Protein Crystallization and Microgravity: Glucose Isomerase Crystals Grown During the Pcdf-Protein Mission within 5.0Å range: probe atom residue distance (Å) B Occ A:Co1391 b:21.1 occ:1.00 OE2 A:GLU217 2.1 12.1 1.0 O A:HOH2283 2.1 15.2 1.0 OD1 A:ASP255 2.2 16.6 1.0 OD1 A:ASP257 2.3 14.2 1.0 OD2 A:ASP255 2.3 16.3 1.0 CG A:ASP255 2.7 13.4 1.0 NE2 A:HIS220 2.7 11.7 1.0 CD A:GLU217 3.1 7.4 1.0 CD2 A:HIS220 3.2 8.5 1.0 CG A:ASP257 3.3 12.3 1.0 O A:HOH2265 3.5 23.5 1.0 OD2 A:ASP257 3.6 18.4 1.0 OE1 A:GLU217 3.7 15.8 1.0 CE1 A:HIS220 3.9 15.3 1.0 ND2 A:ASN247 3.9 6.4 1.0 CB A:ASP255 4.1 11.7 1.0 O A:HOH2312 4.1 11.4 1.0 O A:HOH2262 4.4 25.6 1.0 CG A:GLU217 4.4 6.2 1.0 CG A:HIS220 4.5 6.3 1.0 NZ A:LYS183 4.7 12.2 1.0 CB A:ASP257 4.7 8.7 1.0 OD2 A:ASP287 4.7 11.2 1.0 CE A:LYS183 4.8 7.3 1.0 ND1 A:HIS220 4.8 9.7 1.0 CA A:ASP255 5.0 6.8 1.0 CO A:CO1390 5.0 14.8 1.0

K.Decanniere, L.-D.Patino-Lopez, M.Sleutel, C.Evrard, C.Van De Weerdt, E.Haumont, J.A.Gavira, F.Otalora, D.Maes. Protein Crystallization and Microgravity: Glucose Isomerase Crystals Grown During the Pcdf-Protein Mission To Be Published.