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Cobalt in PDB 4cla: Alternative Binding Modes For Chloramphenicol and 1-Substituted Chloramphenicol Analogues Revealed By Site-Directed Mutagenesis and X-Ray Crystallography of Chloramphenicol Acetyltransferase

Enzymatic activity of Alternative Binding Modes For Chloramphenicol and 1-Substituted Chloramphenicol Analogues Revealed By Site-Directed Mutagenesis and X-Ray Crystallography of Chloramphenicol Acetyltransferase

All present enzymatic activity of Alternative Binding Modes For Chloramphenicol and 1-Substituted Chloramphenicol Analogues Revealed By Site-Directed Mutagenesis and X-Ray Crystallography of Chloramphenicol Acetyltransferase:
2.3.1.28;

Protein crystallography data

The structure of Alternative Binding Modes For Chloramphenicol and 1-Substituted Chloramphenicol Analogues Revealed By Site-Directed Mutagenesis and X-Ray Crystallography of Chloramphenicol Acetyltransferase, PDB code: 4cla was solved by A.G.W.Leslie, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 6.00 / 2.00
Space group H 3 2
Cell size a, b, c (Å), α, β, γ (°) 107.750, 107.750, 123.920, 90.00, 90.00, 120.00
R / Rfree (%) 15.7 / n/a

Other elements in 4cla:

The structure of Alternative Binding Modes For Chloramphenicol and 1-Substituted Chloramphenicol Analogues Revealed By Site-Directed Mutagenesis and X-Ray Crystallography of Chloramphenicol Acetyltransferase also contains other interesting chemical elements:

Chlorine (Cl) 2 atoms

Cobalt Binding Sites:

The binding sites of Cobalt atom in the Alternative Binding Modes For Chloramphenicol and 1-Substituted Chloramphenicol Analogues Revealed By Site-Directed Mutagenesis and X-Ray Crystallography of Chloramphenicol Acetyltransferase (pdb code 4cla). This binding sites where shown within 5.0 Angstroms radius around Cobalt atom.
In total 2 binding sites of Cobalt where determined in the Alternative Binding Modes For Chloramphenicol and 1-Substituted Chloramphenicol Analogues Revealed By Site-Directed Mutagenesis and X-Ray Crystallography of Chloramphenicol Acetyltransferase, PDB code: 4cla:
Jump to Cobalt binding site number: 1; 2;

Cobalt binding site 1 out of 2 in 4cla

Go back to Cobalt Binding Sites List in 4cla
Cobalt binding site 1 out of 2 in the Alternative Binding Modes For Chloramphenicol and 1-Substituted Chloramphenicol Analogues Revealed By Site-Directed Mutagenesis and X-Ray Crystallography of Chloramphenicol Acetyltransferase


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 1 of Alternative Binding Modes For Chloramphenicol and 1-Substituted Chloramphenicol Analogues Revealed By Site-Directed Mutagenesis and X-Ray Crystallography of Chloramphenicol Acetyltransferase within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Co222

b:10.8
occ:0.50
 OE1 A:GLU23 1.9 14.9 1.0
ND1 A:HIS27 2.1 17.3 1.0
CD A:GLU23 2.7 15.0 1.0
OE2 A:GLU23 2.8 13.5 1.0
CE1 A:HIS27 3.0 17.2 1.0
CG A:HIS27 3.2 13.1 1.0
CB A:HIS27 3.6 11.3 1.0
CG A:GLU23 4.1 8.0 1.0
NE2 A:HIS27 4.1 12.0 1.0
O A:HOH266 4.2 40.3 1.0
CD2 A:HIS27 4.3 12.5 1.0
O A:HOH267 4.4 38.8 1.0
O A:HOH255 4.6 27.4 1.0

Cobalt binding site 2 out of 2 in 4cla

Go back to Cobalt Binding Sites List in 4cla
Cobalt binding site 2 out of 2 in the Alternative Binding Modes For Chloramphenicol and 1-Substituted Chloramphenicol Analogues Revealed By Site-Directed Mutagenesis and X-Ray Crystallography of Chloramphenicol Acetyltransferase


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 2 of Alternative Binding Modes For Chloramphenicol and 1-Substituted Chloramphenicol Analogues Revealed By Site-Directed Mutagenesis and X-Ray Crystallography of Chloramphenicol Acetyltransferase within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Co223

b:37.6
occ:0.50
 O A:HOH340 1.9 60.4 0.5
O A:HOH281 2.3 34.8 1.0
O A:HOH426 2.5 52.4 1.0
O A:HOH412 3.8 81.8 1.0
O A:ASN68 4.0 20.7 1.0
O A:ASP87 4.2 19.1 1.0
O A:HOH299 4.2 49.0 1.0
O A:HOH369 4.7 68.6 1.0
O A:HOH351 4.9 57.6 1.0

Reference:

I.A.Murray, A.Lewendon, J.A.Williams, P.M.Cullis, W.V.Shaw. Alternative Binding Modes For Chloramphenicol and 1-Substituted Chloramphenicol Analogues Revealed By Site-Directed Mutagenesis and X-Ray Crystallography of Chloramphenicol Acetyltransferase. Biochemistry V. 30 3763 1991.
ISSN: ISSN 0006-2960
PubMed: 2015231
DOI: 10.1021/BI00229A025
Page generated: Sun Dec 13 10:43:57 2020

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