Cobalt in PDB 4etl: Crystallographic Structure of Phenylalanine Hydroxylase From Chromobacterium Violaceum F258A Mutation

All present enzymatic activity of Crystallographic Structure of Phenylalanine Hydroxylase From Chromobacterium Violaceum F258A Mutation:
1.14.16.1;

The structure of Crystallographic Structure of Phenylalanine Hydroxylase From Chromobacterium Violaceum F258A Mutation, PDB code: 4etl was solved by J.A.Ronau, L.P.Paul, I.R.Corn, K.T.Wagner, M.M.Abu-Omar, C.Das, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	22.68 / 1.49
Space group	P 1
Cell size a, b, c (Å), α, β, γ (°)	36.780, 38.553, 48.044, 76.47, 73.00, 85.36
R / Rfree (%)	16.5 / 21.4

The binding sites of Cobalt atom in the Crystallographic Structure of Phenylalanine Hydroxylase From Chromobacterium Violaceum F258A Mutation (pdb code 4etl). This binding sites where shown within 5.0 Angstroms radius around Cobalt atom.
In total 2 binding sites of Cobalt where determined in the Crystallographic Structure of Phenylalanine Hydroxylase From Chromobacterium Violaceum F258A Mutation, PDB code: 4etl:
Jump to Cobalt binding site number: 1; 2;

Cobalt binding site 1 out of 2 in the Crystallographic Structure of Phenylalanine Hydroxylase From Chromobacterium Violaceum F258A Mutation


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 1 of Crystallographic Structure of Phenylalanine Hydroxylase From Chromobacterium Violaceum F258A Mutation within 5.0Å range: probe atom residue distance (Å) B Occ A:Co301 b:11.9 occ:1.00 NE2 A:HIS138 2.0 9.8 1.0 NE2 A:HIS143 2.1 8.6 1.0 OE2 A:GLU184 2.1 10.5 1.0 O A:HOH481 2.1 13.4 1.0 O A:HOH404 2.1 12.4 1.0 OE1 A:GLU184 2.2 9.7 1.0 CD A:GLU184 2.5 8.9 1.0 CE1 A:HIS138 2.9 10.1 1.0 CD2 A:HIS138 3.0 10.0 1.0 CE1 A:HIS143 3.0 8.9 1.0 CD2 A:HIS143 3.1 9.0 1.0 O A:HOH403 3.9 14.2 1.0 CG A:GLU184 4.0 8.7 1.0 ND1 A:HIS138 4.1 10.2 1.0 OD1 A:ASP139 4.1 13.5 1.0 CG A:HIS138 4.2 9.7 1.0 ND1 A:HIS143 4.2 9.4 1.0 CG A:HIS143 4.3 8.3 1.0 O A:HOH443 4.4 23.9 1.0 O A:HOH449 4.4 19.9 1.0 CB A:ALA199 4.6 8.9 1.0 CB A:GLU184 4.9 8.6 1.0

Cobalt binding site 2 out of 2 in the Crystallographic Structure of Phenylalanine Hydroxylase From Chromobacterium Violaceum F258A Mutation


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 2 of Crystallographic Structure of Phenylalanine Hydroxylase From Chromobacterium Violaceum F258A Mutation within 5.0Å range: probe atom residue distance (Å) B Occ A:Co302 b:39.9 occ:1.00 NE2 A:HIS22 2.0 34.5 1.0 CE1 A:HIS22 2.9 29.4 1.0 O A:HOH495 2.9 30.1 1.0 CD2 A:HIS22 3.1 30.2 1.0 ND1 A:HIS22 4.1 29.9 1.0 CG A:HIS22 4.2 26.6 1.0 O A:HOH560 4.5 41.2 1.0 O A:HOH574 4.6 34.0 1.0 O A:HOH520 4.8 35.0 1.0

J.A.Ronau, L.N.Paul, J.E.Fuchs, I.R.Corn, K.T.Wagner, K.R.Liedl, M.M.Abu-Omar, C.Das. An Additional Substrate Binding Site in A Bacterial Phenylalanine Hydroxylase. Eur.Biophys.J. V. 42 691 2013.
ISSN: ISSN 0175-7571
PubMed: 23860686
DOI: 10.1007/S00249-013-0919-8