Cobalt in PDB 4ft8: E. Coli Catabolite Activator Protein with Cobalt and Sulfate Ligands

The structure of E. Coli Catabolite Activator Protein with Cobalt and Sulfate Ligands, PDB code: 4ft8 was solved by R.Rao, C.L.Lawson, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	40.78 / 1.97
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	104.143, 108.452, 44.320, 90.00, 90.00, 90.00
R / Rfree (%)	19.6 / 23

The binding sites of Cobalt atom in the E. Coli Catabolite Activator Protein with Cobalt and Sulfate Ligands (pdb code 4ft8). This binding sites where shown within 5.0 Angstroms radius around Cobalt atom.
In total 2 binding sites of Cobalt where determined in the E. Coli Catabolite Activator Protein with Cobalt and Sulfate Ligands, PDB code: 4ft8:
Jump to Cobalt binding site number: 1; 2;

Cobalt binding site 1 out of 2 in the E. Coli Catabolite Activator Protein with Cobalt and Sulfate Ligands


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 1 of E. Coli Catabolite Activator Protein with Cobalt and Sulfate Ligands within 5.0Å range: probe atom residue distance (Å) B Occ A:Co308 b:33.0 occ:1.00 NE2 A:HIS21 2.0 20.0 1.0 O A:HOH402 2.2 30.6 1.0 NE2 A:HIS19 2.2 31.7 1.0 OE2 A:GLU96 2.3 30.7 1.0 CE1 A:HIS21 3.0 38.1 1.0 CD2 A:HIS21 3.1 33.1 1.0 CD A:GLU96 3.1 31.6 1.0 CE1 A:HIS19 3.1 36.2 1.0 CD2 A:HIS19 3.3 33.2 1.0 OE1 A:GLU96 3.7 30.5 1.0 CG A:GLU96 3.9 29.5 1.0 O A:HOH410 4.0 33.2 1.0 CB A:GLU96 4.1 28.2 1.0 ND1 A:HIS21 4.1 31.5 1.0 CG A:HIS21 4.2 32.0 1.0 ND1 A:HIS19 4.3 30.5 1.0 O A:HOH584 4.3 38.8 1.0 CG A:HIS19 4.4 32.6 1.0 O A:HOH518 4.5 39.7 1.0

Cobalt binding site 2 out of 2 in the E. Coli Catabolite Activator Protein with Cobalt and Sulfate Ligands


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 2 of E. Coli Catabolite Activator Protein with Cobalt and Sulfate Ligands within 5.0Å range: probe atom residue distance (Å) B Occ B:Co309 b:25.3 occ:1.00 NE2 B:HIS19 2.1 25.8 1.0 NE2 B:HIS21 2.1 25.9 1.0 OE1 B:GLU96 2.2 24.8 1.0 CD2 B:HIS19 3.0 21.0 1.0 CE1 B:HIS19 3.1 24.8 1.0 CD B:GLU96 3.1 28.0 1.0 CE1 B:HIS21 3.1 30.0 1.0 CD2 B:HIS21 3.1 26.1 1.0 OE2 B:GLU96 3.8 27.7 1.0 CG B:GLU96 4.0 21.2 1.0 O B:HOH420 4.0 23.1 1.0 CB B:GLU96 4.1 20.8 1.0 ND1 B:HIS19 4.2 29.2 1.0 CG B:HIS19 4.2 26.3 1.0 ND1 B:HIS21 4.2 30.7 1.0 CG B:HIS21 4.3 28.1 1.0 O B:HOH454 4.5 32.8 1.0

R.R.Rao, C.L.Lawson. Structure of Catabolite Activator Protein with Cobalt(II) and Sulfate. Acta Crystallogr F Struct V. 70 560 2014BIOL Commun.
ISSN: ISSN 1744-3091
PubMed: 24817710
DOI: 10.1107/S2053230X14005366