Atomistry » Cobalt » PDB 4fe5-4j0b » 4hhm
Atomistry »
  Cobalt »
    PDB 4fe5-4j0b »
      4hhm »

Cobalt in PDB 4hhm: Crystal Structure of A Mutant, G219A, of Glucose Isomerase From Streptomyces Sp. Sk

Enzymatic activity of Crystal Structure of A Mutant, G219A, of Glucose Isomerase From Streptomyces Sp. Sk

All present enzymatic activity of Crystal Structure of A Mutant, G219A, of Glucose Isomerase From Streptomyces Sp. Sk:
5.3.1.5;

Protein crystallography data

The structure of Crystal Structure of A Mutant, G219A, of Glucose Isomerase From Streptomyces Sp. Sk, PDB code: 4hhm was solved by H.Ben Hlima, J.Riguet, R.Haser, N.Aghajari, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 48.50 / 2.15
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 85.500, 137.400, 136.600, 90.00, 90.06, 90.00
R / Rfree (%) 19.7 / 25

Other elements in 4hhm:

The structure of Crystal Structure of A Mutant, G219A, of Glucose Isomerase From Streptomyces Sp. Sk also contains other interesting chemical elements:

Magnesium (Mg) 8 atoms

Cobalt Binding Sites:

The binding sites of Cobalt atom in the Crystal Structure of A Mutant, G219A, of Glucose Isomerase From Streptomyces Sp. Sk (pdb code 4hhm). This binding sites where shown within 5.0 Angstroms radius around Cobalt atom.
In total 8 binding sites of Cobalt where determined in the Crystal Structure of A Mutant, G219A, of Glucose Isomerase From Streptomyces Sp. Sk, PDB code: 4hhm:
Jump to Cobalt binding site number: 1; 2; 3; 4; 5; 6; 7; 8;

Cobalt binding site 1 out of 8 in 4hhm

Go back to Cobalt Binding Sites List in 4hhm
Cobalt binding site 1 out of 8 in the Crystal Structure of A Mutant, G219A, of Glucose Isomerase From Streptomyces Sp. Sk


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 1 of Crystal Structure of A Mutant, G219A, of Glucose Isomerase From Streptomyces Sp. Sk within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Co2001

b:47.9
occ:1.00
OE2 A:GLU217 2.0 23.6 1.0
OD2 A:ASP255 2.1 25.7 1.0
OD1 A:ASP257 2.2 21.9 1.0
CG A:ASP255 3.0 27.5 1.0
CG A:ASP257 3.1 21.4 1.0
OD1 A:ASP255 3.1 26.8 1.0
CD A:GLU217 3.2 22.2 1.0
O A:HOH2260 3.2 16.2 1.0
OD2 A:ASP257 3.3 25.0 1.0
NE2 A:HIS220 3.3 15.1 1.0
OE1 A:GLU217 3.7 23.7 1.0
CD2 A:HIS220 3.8 10.7 1.0
ND2 A:ASN247 4.0 16.0 1.0
CG A:GLU217 4.4 19.9 1.0
OD2 A:ASP287 4.4 20.0 1.0
CB A:ASP255 4.4 22.3 1.0
CE1 A:HIS220 4.4 10.3 1.0
CB A:ASP257 4.5 19.3 1.0
OD1 A:ASP287 4.5 22.0 1.0
CG A:ASP287 4.5 20.0 1.0
O A:ASP287 4.5 21.6 1.0
MG A:MG2002 4.6 11.9 1.0
CB A:ALA219 4.9 10.3 1.0
NZ A:LYS289 5.0 28.2 1.0

Cobalt binding site 2 out of 8 in 4hhm

Go back to Cobalt Binding Sites List in 4hhm
Cobalt binding site 2 out of 8 in the Crystal Structure of A Mutant, G219A, of Glucose Isomerase From Streptomyces Sp. Sk


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 2 of Crystal Structure of A Mutant, G219A, of Glucose Isomerase From Streptomyces Sp. Sk within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Co2001

b:35.2
occ:1.00
OD2 B:ASP255 2.1 24.2 1.0
OE2 B:GLU217 2.2 10.1 1.0
OD1 B:ASP257 2.3 19.7 1.0
CG B:ASP255 2.7 26.1 1.0
OD1 B:ASP255 2.8 27.1 1.0
O B:HOH2251 3.0 12.5 1.0
CG B:ASP257 3.1 17.4 1.0
OD2 B:ASP257 3.1 22.8 1.0
CD B:GLU217 3.2 12.8 1.0
NE2 B:HIS220 3.2 14.1 1.0
OE1 B:GLU217 3.5 13.1 1.0
CD2 B:HIS220 3.7 13.8 1.0
O B:HOH2337 4.0 27.0 1.0
ND2 B:ASN247 4.0 6.8 1.0
CB B:ASP255 4.1 21.7 1.0
CE1 B:HIS220 4.3 13.0 1.0
OD2 B:ASP287 4.4 23.3 1.0
CB B:ASP257 4.5 13.6 1.0
CG B:GLU217 4.5 14.3 1.0
MG B:MG2002 4.6 18.1 1.0
CB B:ALA219 4.7 8.3 1.0
CG B:ASP287 4.8 18.8 1.0
O B:HOH2401 4.8 39.6 1.0
NZ B:LYS183 4.9 17.3 1.0
O B:ASP287 4.9 18.9 1.0
O B:HOH2168 4.9 27.9 1.0
OD1 B:ASP287 4.9 20.1 1.0
CG B:HIS220 5.0 14.4 1.0

Cobalt binding site 3 out of 8 in 4hhm

Go back to Cobalt Binding Sites List in 4hhm
Cobalt binding site 3 out of 8 in the Crystal Structure of A Mutant, G219A, of Glucose Isomerase From Streptomyces Sp. Sk


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 3 of Crystal Structure of A Mutant, G219A, of Glucose Isomerase From Streptomyces Sp. Sk within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Co2001

b:39.3
occ:1.00
OD2 C:ASP255 2.1 26.8 1.0
OE2 C:GLU217 2.2 15.4 1.0
OD1 C:ASP257 2.4 27.9 1.0
OD2 C:ASP257 2.9 29.5 1.0
CG C:ASP255 3.0 26.8 1.0
CG C:ASP257 3.0 24.9 1.0
CD C:GLU217 3.0 15.9 1.0
OE1 C:GLU217 3.1 21.9 1.0
O C:HOH2314 3.2 32.7 1.0
OD1 C:ASP255 3.2 29.3 1.0
NE2 C:HIS220 3.5 18.2 1.0
ND2 C:ASN247 3.8 18.7 1.0
CD2 C:HIS220 3.9 15.0 1.0
O C:HOH2315 4.1 29.5 1.0
OD2 C:ASP287 4.3 18.0 1.0
CB C:ASP255 4.3 22.4 1.0
CG C:GLU217 4.4 15.8 1.0
CB C:ASP257 4.4 22.3 1.0
O C:ASP287 4.5 22.6 1.0
CB C:ALA219 4.6 15.4 1.0
CE1 C:HIS220 4.7 17.2 1.0
CG C:ASP287 4.7 22.2 1.0
MG C:MG2002 4.8 15.4 1.0
OD1 C:ASP287 4.9 24.0 1.0
CA C:ASP257 4.9 19.4 1.0

Cobalt binding site 4 out of 8 in 4hhm

Go back to Cobalt Binding Sites List in 4hhm
Cobalt binding site 4 out of 8 in the Crystal Structure of A Mutant, G219A, of Glucose Isomerase From Streptomyces Sp. Sk


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 4 of Crystal Structure of A Mutant, G219A, of Glucose Isomerase From Streptomyces Sp. Sk within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Co2001

b:40.8
occ:1.00
OD1 D:ASP257 2.1 19.1 1.0
OE2 D:GLU217 2.2 20.9 1.0
OD2 D:ASP255 2.2 25.7 1.0
CG D:ASP255 2.9 25.1 1.0
CD D:GLU217 3.0 19.2 1.0
CG D:ASP257 3.0 17.6 1.0
OD1 D:ASP255 3.0 26.0 1.0
OE1 D:GLU217 3.0 21.1 1.0
NE2 D:HIS220 3.2 14.4 1.0
OD2 D:ASP257 3.2 21.7 1.0
CD2 D:HIS220 3.6 11.7 1.0
ND2 D:ASN247 3.9 17.4 1.0
O D:HOH2255 3.9 51.9 1.0
OD1 D:ASP287 4.1 19.9 1.0
CE1 D:HIS220 4.3 12.0 1.0
CB D:ASP255 4.4 20.4 1.0
CB D:ASP257 4.4 16.2 1.0
CG D:GLU217 4.4 19.9 1.0
CG D:ASP287 4.5 16.5 1.0
MG D:MG2002 4.5 17.9 1.0
O D:ASP287 4.6 19.0 1.0
OD2 D:ASP287 4.7 17.7 1.0
CB D:ALA219 4.7 12.2 1.0
NZ D:LYS289 4.9 29.4 1.0
CG D:HIS220 4.9 13.3 1.0
CA D:ASP257 4.9 16.2 1.0

Cobalt binding site 5 out of 8 in 4hhm

Go back to Cobalt Binding Sites List in 4hhm
Cobalt binding site 5 out of 8 in the Crystal Structure of A Mutant, G219A, of Glucose Isomerase From Streptomyces Sp. Sk


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 5 of Crystal Structure of A Mutant, G219A, of Glucose Isomerase From Streptomyces Sp. Sk within 5.0Å range:
probe atom residue distance (Å) B Occ
E:Co2001

b:40.2
occ:1.00
OD2 E:ASP255 2.2 24.0 1.0
OE2 E:GLU217 2.2 15.1 1.0
OD1 E:ASP257 2.3 21.4 1.0
OE1 E:GLU217 2.7 16.8 1.0
O E:HOH2359 2.7 36.5 1.0
CD E:GLU217 2.8 13.0 1.0
NE2 E:HIS220 3.1 16.9 1.0
CG E:ASP255 3.1 25.3 1.0
CG E:ASP257 3.3 21.0 1.0
OD1 E:ASP255 3.4 22.1 1.0
OD2 E:ASP257 3.5 27.5 1.0
CD2 E:HIS220 3.6 14.4 1.0
O E:HOH2261 3.7 30.4 1.0
ND2 E:ASN247 3.9 11.5 1.0
CE1 E:HIS220 4.0 16.3 1.0
MG E:MG2002 4.2 17.8 1.0
CG E:GLU217 4.3 13.2 1.0
OD2 E:ASP287 4.5 21.6 1.0
CB E:ASP255 4.5 18.1 1.0
OD1 E:ASP287 4.5 24.1 1.0
CG E:ASP287 4.6 23.2 1.0
O E:ASP287 4.7 19.0 1.0
CB E:ASP257 4.7 16.2 1.0
CG E:HIS220 4.8 16.3 1.0
ND1 E:HIS220 5.0 16.9 1.0
CB E:ALA219 5.0 8.9 1.0

Cobalt binding site 6 out of 8 in 4hhm

Go back to Cobalt Binding Sites List in 4hhm
Cobalt binding site 6 out of 8 in the Crystal Structure of A Mutant, G219A, of Glucose Isomerase From Streptomyces Sp. Sk


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 6 of Crystal Structure of A Mutant, G219A, of Glucose Isomerase From Streptomyces Sp. Sk within 5.0Å range:
probe atom residue distance (Å) B Occ
F:Co2001

b:40.1
occ:1.00
OE2 F:GLU217 2.0 17.8 1.0
OD1 F:ASP257 2.1 24.1 1.0
OD2 F:ASP255 2.1 21.2 1.0
CG F:ASP255 2.9 25.1 1.0
CD F:GLU217 2.9 18.3 1.0
OD1 F:ASP255 3.0 24.8 1.0
CG F:ASP257 3.0 26.0 1.0
OE1 F:GLU217 3.1 21.9 1.0
OD2 F:ASP257 3.3 29.6 1.0
NE2 F:HIS220 3.5 19.6 1.0
ND2 F:ASN247 3.8 11.4 1.0
CD2 F:HIS220 3.9 16.6 1.0
O F:HOH2210 4.3 16.0 1.0
CG F:GLU217 4.3 17.5 1.0
CB F:ASP257 4.4 24.6 1.0
CB F:ASP255 4.4 21.9 1.0
O F:ASP287 4.5 21.8 1.0
CB F:ALA219 4.5 15.0 1.0
OD1 F:ASP287 4.6 24.2 1.0
CE1 F:HIS220 4.6 16.8 1.0
OD2 F:ASP287 4.7 19.9 1.0
CG F:ASP287 4.7 18.4 1.0
CA F:ASP257 4.8 21.2 1.0
MG F:MG2002 4.9 21.0 1.0

Cobalt binding site 7 out of 8 in 4hhm

Go back to Cobalt Binding Sites List in 4hhm
Cobalt binding site 7 out of 8 in the Crystal Structure of A Mutant, G219A, of Glucose Isomerase From Streptomyces Sp. Sk


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 7 of Crystal Structure of A Mutant, G219A, of Glucose Isomerase From Streptomyces Sp. Sk within 5.0Å range:
probe atom residue distance (Å) B Occ
G:Co2001

b:40.3
occ:1.00
OD1 G:ASP257 2.1 22.5 1.0
OD2 G:ASP255 2.2 20.1 1.0
OE2 G:GLU217 2.3 24.4 1.0
CD G:GLU217 3.0 19.7 1.0
OE1 G:GLU217 3.0 16.7 1.0
CG G:ASP255 3.0 25.2 1.0
CG G:ASP257 3.1 22.9 1.0
OD1 G:ASP255 3.2 23.8 1.0
NE2 G:HIS220 3.3 18.8 1.0
OD2 G:ASP257 3.4 24.3 1.0
ND2 G:ASN247 3.8 19.6 1.0
CD2 G:HIS220 3.8 18.1 1.0
O G:HOH2262 3.9 24.2 1.0
OD2 G:ASP287 4.2 20.0 1.0
MG G:MG2002 4.4 13.0 1.0
CE1 G:HIS220 4.4 17.8 1.0
CG G:GLU217 4.4 19.4 1.0
CB G:ASP257 4.4 19.9 1.0
CB G:ASP255 4.5 19.3 1.0
CG G:ASP287 4.5 19.5 1.0
O G:ASP287 4.5 21.2 1.0
OD1 G:ASP287 4.5 20.2 1.0
CB G:ALA219 4.7 9.1 1.0
CA G:ASP257 4.9 20.6 1.0

Cobalt binding site 8 out of 8 in 4hhm

Go back to Cobalt Binding Sites List in 4hhm
Cobalt binding site 8 out of 8 in the Crystal Structure of A Mutant, G219A, of Glucose Isomerase From Streptomyces Sp. Sk


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 8 of Crystal Structure of A Mutant, G219A, of Glucose Isomerase From Streptomyces Sp. Sk within 5.0Å range:
probe atom residue distance (Å) B Occ
H:Co2001

b:37.6
occ:1.00
OD2 H:ASP255 2.1 17.7 1.0
OE2 H:GLU217 2.3 20.8 1.0
OD1 H:ASP257 2.3 24.6 1.0
O H:HOH2387 2.8 30.6 1.0
CD H:GLU217 3.0 18.5 1.0
OE1 H:GLU217 3.0 16.6 1.0
CG H:ASP255 3.0 21.9 1.0
CG H:ASP257 3.1 21.8 1.0
O H:HOH2277 3.2 34.6 1.0
OD2 H:ASP257 3.2 25.6 1.0
OD1 H:ASP255 3.2 22.2 1.0
NE2 H:HIS220 3.4 11.7 1.0
ND2 H:ASN247 3.8 11.1 1.0
CD2 H:HIS220 3.9 11.7 1.0
OD2 H:ASP287 4.0 15.3 1.0
CB H:ASP255 4.4 16.6 1.0
CE1 H:HIS220 4.4 11.0 1.0
CG H:GLU217 4.4 16.6 1.0
CB H:ASP257 4.5 17.4 1.0
CG H:ASP287 4.6 17.0 1.0
MG H:MG2002 4.6 21.7 1.0
O H:ASP287 4.6 17.9 1.0
O H:HOH2249 4.7 30.7 1.0
CB H:ALA219 4.8 7.0 1.0
OD1 H:ASP287 4.9 17.3 1.0

Reference:

H.Ben Hlima, S.Bejar, J.Riguet, R.Haser, N.Aghajari. Identification of Critical Residues For the Activity and Thermostability of Streptomyces Sp. Sk Glucose Isomerase. Appl.Microbiol.Biotechnol. V. 97 9715 2013.
ISSN: ISSN 0175-7598
PubMed: 23463249
DOI: 10.1007/S00253-013-4784-2
Page generated: Tue Jul 30 17:09:38 2024

Last articles

Al in 7NIC
Al in 7NIQ
Al in 7L07
Al in 7N77
Al in 7N73
Al in 7N72
Al in 7LVR
Al in 7KYB
Al in 7JL3
Al in 7KRO
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy