Cobalt in PDB 4i2g: Binary Complex of Mouse Tdt with Ssdna

All present enzymatic activity of Binary Complex of Mouse Tdt with Ssdna:
2.7.7.31;

The structure of Binary Complex of Mouse Tdt with Ssdna, PDB code: 4i2g was solved by J.Gouge, M.Delarue, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	23.29 / 2.50
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	46.826, 84.636, 115.850, 90.00, 90.00, 90.00
R / Rfree (%)	18.4 / 21.8

The structure of Binary Complex of Mouse Tdt with Ssdna also contains other interesting chemical elements:

Magnesium	(Mg)	1 atom
Sodium	(Na)	1 atom

The binding sites of Cobalt atom in the Binary Complex of Mouse Tdt with Ssdna (pdb code 4i2g). This binding sites where shown within 5.0 Angstroms radius around Cobalt atom.
In total only one binding site of Cobalt was determined in the Binary Complex of Mouse Tdt with Ssdna, PDB code: 4i2g:

Cobalt binding site 1 out of 1 in the Binary Complex of Mouse Tdt with Ssdna


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 1 of Binary Complex of Mouse Tdt with Ssdna within 5.0Å range: probe atom residue distance (Å) B Occ A:Co601 b:55.3 occ:1.00 OP1 C:DA5 1.9 0.4 1.0 OD2 A:ASP343 2.0 44.0 1.0 OD2 A:ASP434 2.1 44.5 1.0 OD1 A:ASP345 2.3 64.6 1.0 OD2 A:ASP345 2.8 55.6 1.0 CG A:ASP345 2.8 55.8 1.0 CG A:ASP343 2.9 41.4 1.0 P C:DA5 3.0 0.6 1.0 OD1 A:ASP343 3.1 44.0 1.0 O3' C:DA4 3.2 0.9 1.0 CG A:ASP434 3.3 45.6 1.0 C5' C:DA4 3.7 0.1 1.0 MG A:MG603 3.9 38.7 1.0 O5' C:DA5 3.9 0.8 1.0 CB A:ASP434 4.0 41.8 1.0 O5' C:DA4 4.0 0.0 1.0 C3' C:DA4 4.1 0.9 1.0 OP2 C:DA5 4.2 0.8 1.0 CB A:ASP345 4.2 43.6 1.0 CB A:ASP343 4.2 34.1 1.0 C4' C:DA4 4.2 1.0 1.0 OD1 A:ASP434 4.3 47.6 1.0 C5' C:DA5 4.5 0.8 1.0 CZ3 A:TRP450 4.7 46.9 1.0 NH2 A:ARG432 4.8 41.8 1.0

J.Gouge, S.Rosario, F.Romain, P.Beguin, M.Delarue. Structures of Intermediates Along the Catalytic Cycle of Terminal Deoxynucleotidyltransferase: Dynamical Aspects of the Two-Metal Ion Mechanism. J.Mol.Biol. V. 425 4334 2013.
ISSN: ISSN 0022-2836
PubMed: 23856622
DOI: 10.1016/J.JMB.2013.07.009