Cobalt in PDB 4v0z: O-Nitrophenyl Cellobioside As An Active Site Probe For Family 7 Cellobiohydrolases

All present enzymatic activity of O-Nitrophenyl Cellobioside As An Active Site Probe For Family 7 Cellobiohydrolases:
3.2.1.176;

The structure of O-Nitrophenyl Cellobioside As An Active Site Probe For Family 7 Cellobiohydrolases, PDB code: 4v0z was solved by A.Nutt, M.H.Momeni, J.Stahlberg, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	14.94 / 1.70
Space group	I 2 2 2
Cell size a, b, c (Å), α, β, γ (°)	83.063, 81.375, 109.942, 90.00, 90.00, 90.00
R / Rfree (%)	12.909 / 15.664

The binding sites of Cobalt atom in the O-Nitrophenyl Cellobioside As An Active Site Probe For Family 7 Cellobiohydrolases (pdb code 4v0z). This binding sites where shown within 5.0 Angstroms radius around Cobalt atom.
In total 2 binding sites of Cobalt where determined in the O-Nitrophenyl Cellobioside As An Active Site Probe For Family 7 Cellobiohydrolases, PDB code: 4v0z:
Jump to Cobalt binding site number: 1; 2;

Cobalt binding site 1 out of 2 in the O-Nitrophenyl Cellobioside As An Active Site Probe For Family 7 Cellobiohydrolases


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 1 of O-Nitrophenyl Cellobioside As An Active Site Probe For Family 7 Cellobiohydrolases within 5.0Å range: probe atom residue distance (Å) B Occ A:Co436 b:5.1 occ:0.50 OE2 A:GLU325 2.1 5.1 1.0 OE1 A:GLU295 2.1 4.1 1.0 OE2 A:GLU295 2.2 4.1 1.0 CD A:GLU295 2.5 4.2 1.0 CD A:GLU325 3.1 5.2 1.0 OE1 A:GLU325 3.5 5.3 1.0 O A:HOH2418 3.9 9.1 1.0 CG A:GLU295 4.0 4.3 1.0 ND2 A:ASN301 4.0 4.7 1.0 O A:HOH2392 4.3 7.3 1.0 CG A:GLU325 4.4 5.2 1.0 OG A:SER297 4.7 5.3 1.0 CB A:ALA299 4.8 4.2 1.0 CB A:GLU295 4.9 4.5 1.0 O A:HOH2419 5.0 6.0 1.0

Cobalt binding site 2 out of 2 in the O-Nitrophenyl Cellobioside As An Active Site Probe For Family 7 Cellobiohydrolases


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 2 of O-Nitrophenyl Cellobioside As An Active Site Probe For Family 7 Cellobiohydrolases within 5.0Å range: probe atom residue distance (Å) B Occ A:Co437 b:36.2 occ:1.00 O A:HOH2294 1.6 18.5 1.0 OE2 A:GLU239 1.8 22.5 0.8 O A:HOH2293 2.1 33.8 1.0 O A:HOH2295 2.1 17.5 1.0 NE2 A:HIS206 2.2 8.1 0.5 NE2 A:HIS206 2.2 8.6 0.5 CD A:GLU239 2.7 19.2 1.0 CD2 A:HIS206 2.9 8.1 0.5 CE1 A:HIS206 2.9 8.6 0.5 OE1 A:GLU239 3.1 22.0 0.8 CE1 A:HIS206 3.4 8.2 0.5 CD2 A:HIS206 3.4 8.5 0.5 CG A:GLU239 4.0 16.7 1.0 ND1 A:HIS206 4.1 8.5 0.5 CG A:HIS206 4.2 7.7 0.5 ND1 A:HIS206 4.4 8.0 0.5 CG A:HIS206 4.4 8.0 0.5 O A:HOH2114 4.8 31.1 1.0

A.Nutt, M.Nilsson, P.Valjamae, M.H.Momeni, J.Stahlberg, R.Isaksson, G.Johansson. O-Nitrophenyl Cellobioside As An Active Site Probe For Family 7 Cellobiohydrolases To Be Published.