Cobalt in PDB 5d0a: Crystal Structure of Epoxyqueuosine Reductase with Cleaved Rna Stem Loop
Enzymatic activity of Crystal Structure of Epoxyqueuosine Reductase with Cleaved Rna Stem Loop
All present enzymatic activity of Crystal Structure of Epoxyqueuosine Reductase with Cleaved Rna Stem Loop:
1.17.99.6;
Protein crystallography data
The structure of Crystal Structure of Epoxyqueuosine Reductase with Cleaved Rna Stem Loop, PDB code: 5d0a
was solved by
D.P.Dowling,
Z.D.Miles,
C.Kohrer,
V.Bandarian,
C.L.Drennan,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
46.39 /
2.10
|
Space group
|
P 1 21 1
|
Cell size a, b, c (Å), α, β, γ (°)
|
90.587,
111.118,
96.008,
90.00,
104.90,
90.00
|
R / Rfree (%)
|
18.4 /
22.5
|
Other elements in 5d0a:
The structure of Crystal Structure of Epoxyqueuosine Reductase with Cleaved Rna Stem Loop also contains other interesting chemical elements:
Cobalt Binding Sites:
The binding sites of Cobalt atom in the Crystal Structure of Epoxyqueuosine Reductase with Cleaved Rna Stem Loop
(pdb code 5d0a). This binding sites where shown within
5.0 Angstroms radius around Cobalt atom.
In total 4 binding sites of Cobalt where determined in the
Crystal Structure of Epoxyqueuosine Reductase with Cleaved Rna Stem Loop, PDB code: 5d0a:
Jump to Cobalt binding site number:
1;
2;
3;
4;
Cobalt binding site 1 out
of 4 in 5d0a
Go back to
Cobalt Binding Sites List in 5d0a
Cobalt binding site 1 out
of 4 in the Crystal Structure of Epoxyqueuosine Reductase with Cleaved Rna Stem Loop
Mono view
Stereo pair view
|
A full contact list of Cobalt with other atoms in the Co binding
site number 1 of Crystal Structure of Epoxyqueuosine Reductase with Cleaved Rna Stem Loop within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Co503
b:25.4
occ:1.00
|
CO
|
A:B12503
|
0.0
|
25.4
|
1.0
|
N21
|
A:B12503
|
1.9
|
24.3
|
1.0
|
N24
|
A:B12503
|
1.9
|
29.1
|
1.0
|
N22
|
A:B12503
|
1.9
|
28.2
|
1.0
|
N23
|
A:B12503
|
1.9
|
27.5
|
1.0
|
O
|
A:HOH652
|
2.6
|
28.4
|
1.0
|
C1
|
A:B12503
|
2.8
|
22.6
|
1.0
|
C19
|
A:B12503
|
2.8
|
23.8
|
1.0
|
C9
|
A:B12503
|
2.9
|
30.2
|
1.0
|
C4
|
A:B12503
|
2.9
|
25.4
|
1.0
|
C11
|
A:B12503
|
2.9
|
27.7
|
1.0
|
C14
|
A:B12503
|
3.0
|
26.1
|
1.0
|
C6
|
A:B12503
|
3.0
|
26.4
|
1.0
|
C16
|
A:B12503
|
3.0
|
26.4
|
1.0
|
C20
|
A:B12503
|
3.3
|
24.9
|
1.0
|
C10
|
A:B12503
|
3.3
|
22.5
|
1.0
|
C5
|
A:B12503
|
3.3
|
27.8
|
1.0
|
C15
|
A:B12503
|
3.4
|
26.6
|
1.0
|
C18
|
A:B12503
|
4.1
|
22.0
|
1.0
|
C2
|
A:B12503
|
4.2
|
22.1
|
1.0
|
O
|
A:HOH704
|
4.2
|
32.2
|
1.0
|
C8
|
A:B12503
|
4.2
|
25.7
|
1.0
|
C3
|
A:B12503
|
4.2
|
22.9
|
1.0
|
C7
|
A:B12503
|
4.2
|
28.6
|
1.0
|
C13
|
A:B12503
|
4.2
|
26.7
|
1.0
|
C17
|
A:B12503
|
4.3
|
22.6
|
1.0
|
C12
|
A:B12503
|
4.3
|
31.3
|
1.0
|
C49
|
A:B12503
|
4.3
|
26.8
|
1.0
|
O2
|
A:GOL504
|
4.4
|
25.2
|
1.0
|
O
|
A:HOH770
|
4.5
|
31.5
|
1.0
|
C26
|
A:B12503
|
4.7
|
24.9
|
1.0
|
C35
|
A:B12503
|
4.8
|
22.8
|
1.0
|
C37
|
A:B12503
|
4.8
|
25.9
|
1.0
|
C48
|
A:B12503
|
4.8
|
27.8
|
1.0
|
C41
|
A:B12503
|
4.9
|
27.0
|
1.0
|
C53
|
A:B12503
|
4.9
|
23.9
|
1.0
|
C46
|
A:B12503
|
4.9
|
25.6
|
1.0
|
NH2
|
A:ARG141
|
4.9
|
26.4
|
1.0
|
|
Cobalt binding site 2 out
of 4 in 5d0a
Go back to
Cobalt Binding Sites List in 5d0a
Cobalt binding site 2 out
of 4 in the Crystal Structure of Epoxyqueuosine Reductase with Cleaved Rna Stem Loop
Mono view
Stereo pair view
|
A full contact list of Cobalt with other atoms in the Co binding
site number 2 of Crystal Structure of Epoxyqueuosine Reductase with Cleaved Rna Stem Loop within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Co503
b:27.3
occ:1.00
|
CO
|
B:B12503
|
0.0
|
27.3
|
1.0
|
N21
|
B:B12503
|
1.9
|
29.7
|
1.0
|
N24
|
B:B12503
|
1.9
|
28.6
|
1.0
|
N23
|
B:B12503
|
1.9
|
27.5
|
1.0
|
N22
|
B:B12503
|
1.9
|
27.1
|
1.0
|
O
|
B:HOH677
|
2.6
|
30.3
|
1.0
|
C1
|
B:B12503
|
2.8
|
27.9
|
1.0
|
C19
|
B:B12503
|
2.8
|
28.3
|
1.0
|
C9
|
B:B12503
|
2.9
|
30.7
|
1.0
|
C11
|
B:B12503
|
2.9
|
24.7
|
1.0
|
C4
|
B:B12503
|
2.9
|
30.3
|
1.0
|
C14
|
B:B12503
|
3.0
|
23.5
|
1.0
|
C16
|
B:B12503
|
3.0
|
25.2
|
1.0
|
C6
|
B:B12503
|
3.0
|
27.2
|
1.0
|
C10
|
B:B12503
|
3.3
|
22.5
|
1.0
|
C20
|
B:B12503
|
3.3
|
25.2
|
1.0
|
C5
|
B:B12503
|
3.3
|
29.2
|
1.0
|
C15
|
B:B12503
|
3.4
|
21.1
|
1.0
|
C18
|
B:B12503
|
4.1
|
26.3
|
1.0
|
O
|
B:HOH696
|
4.1
|
29.2
|
1.0
|
C2
|
B:B12503
|
4.2
|
27.7
|
1.0
|
C3
|
B:B12503
|
4.2
|
26.2
|
1.0
|
C12
|
B:B12503
|
4.2
|
26.9
|
1.0
|
C8
|
B:B12503
|
4.2
|
26.0
|
1.0
|
C13
|
B:B12503
|
4.2
|
25.9
|
1.0
|
C7
|
B:B12503
|
4.2
|
27.5
|
1.0
|
C17
|
B:B12503
|
4.3
|
22.4
|
1.0
|
C49
|
B:B12503
|
4.3
|
25.8
|
1.0
|
O2
|
B:GOL504
|
4.4
|
29.5
|
1.0
|
O
|
B:HOH783
|
4.7
|
28.2
|
1.0
|
C26
|
B:B12503
|
4.8
|
26.0
|
1.0
|
C35
|
B:B12503
|
4.8
|
27.6
|
1.0
|
C48
|
B:B12503
|
4.8
|
26.9
|
1.0
|
C37
|
B:B12503
|
4.8
|
22.4
|
1.0
|
C41
|
B:B12503
|
4.9
|
26.0
|
1.0
|
C46
|
B:B12503
|
4.9
|
28.1
|
1.0
|
NH2
|
B:ARG141
|
4.9
|
27.3
|
1.0
|
C53
|
B:B12503
|
4.9
|
26.2
|
1.0
|
C3
|
B:GOL504
|
4.9
|
30.7
|
1.0
|
C2
|
B:GOL504
|
5.0
|
29.8
|
1.0
|
C30
|
B:B12503
|
5.0
|
25.4
|
1.0
|
|
Cobalt binding site 3 out
of 4 in 5d0a
Go back to
Cobalt Binding Sites List in 5d0a
Cobalt binding site 3 out
of 4 in the Crystal Structure of Epoxyqueuosine Reductase with Cleaved Rna Stem Loop
Mono view
Stereo pair view
|
A full contact list of Cobalt with other atoms in the Co binding
site number 3 of Crystal Structure of Epoxyqueuosine Reductase with Cleaved Rna Stem Loop within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:Co503
b:26.2
occ:1.00
|
CO
|
C:B12503
|
0.0
|
26.2
|
1.0
|
N21
|
C:B12503
|
1.9
|
27.2
|
1.0
|
N24
|
C:B12503
|
1.9
|
28.3
|
1.0
|
N22
|
C:B12503
|
1.9
|
27.9
|
1.0
|
N23
|
C:B12503
|
1.9
|
24.4
|
1.0
|
O
|
C:HOH678
|
2.5
|
27.9
|
1.0
|
C1
|
C:B12503
|
2.8
|
26.7
|
1.0
|
C19
|
C:B12503
|
2.8
|
24.9
|
1.0
|
C9
|
C:B12503
|
2.9
|
28.7
|
1.0
|
C4
|
C:B12503
|
2.9
|
24.5
|
1.0
|
C11
|
C:B12503
|
2.9
|
21.4
|
1.0
|
C14
|
C:B12503
|
2.9
|
25.7
|
1.0
|
C16
|
C:B12503
|
3.0
|
24.6
|
1.0
|
C6
|
C:B12503
|
3.0
|
28.6
|
1.0
|
C10
|
C:B12503
|
3.3
|
24.4
|
1.0
|
C20
|
C:B12503
|
3.3
|
22.8
|
1.0
|
C5
|
C:B12503
|
3.3
|
25.6
|
1.0
|
C15
|
C:B12503
|
3.4
|
26.9
|
1.0
|
C18
|
C:B12503
|
4.1
|
23.4
|
1.0
|
C2
|
C:B12503
|
4.1
|
27.5
|
1.0
|
O
|
C:HOH700
|
4.2
|
29.5
|
1.0
|
C3
|
C:B12503
|
4.2
|
21.9
|
1.0
|
C49
|
C:B12503
|
4.2
|
24.0
|
1.0
|
C12
|
C:B12503
|
4.2
|
25.7
|
1.0
|
C8
|
C:B12503
|
4.2
|
27.7
|
1.0
|
C13
|
C:B12503
|
4.2
|
29.6
|
1.0
|
C17
|
C:B12503
|
4.3
|
25.3
|
1.0
|
C7
|
C:B12503
|
4.3
|
28.7
|
1.0
|
O2
|
C:GOL504
|
4.3
|
31.6
|
1.0
|
O
|
C:HOH780
|
4.5
|
28.7
|
1.0
|
C26
|
C:B12503
|
4.7
|
25.5
|
1.0
|
C35
|
C:B12503
|
4.8
|
25.6
|
1.0
|
C41
|
C:B12503
|
4.8
|
26.6
|
1.0
|
C37
|
C:B12503
|
4.8
|
25.9
|
1.0
|
C46
|
C:B12503
|
4.8
|
22.5
|
1.0
|
C48
|
C:B12503
|
4.8
|
27.1
|
1.0
|
C53
|
C:B12503
|
4.9
|
21.1
|
1.0
|
C2
|
C:GOL504
|
4.9
|
29.4
|
1.0
|
C30
|
C:B12503
|
4.9
|
25.6
|
1.0
|
C3
|
C:GOL504
|
4.9
|
30.2
|
1.0
|
NH2
|
C:ARG141
|
5.0
|
26.2
|
1.0
|
|
Cobalt binding site 4 out
of 4 in 5d0a
Go back to
Cobalt Binding Sites List in 5d0a
Cobalt binding site 4 out
of 4 in the Crystal Structure of Epoxyqueuosine Reductase with Cleaved Rna Stem Loop
Mono view
Stereo pair view
|
A full contact list of Cobalt with other atoms in the Co binding
site number 4 of Crystal Structure of Epoxyqueuosine Reductase with Cleaved Rna Stem Loop within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
D:Co503
b:26.1
occ:1.00
|
CO
|
D:B12503
|
0.0
|
26.1
|
1.0
|
N21
|
D:B12503
|
1.9
|
26.6
|
1.0
|
N24
|
D:B12503
|
1.9
|
28.5
|
1.0
|
N22
|
D:B12503
|
1.9
|
27.5
|
1.0
|
N23
|
D:B12503
|
1.9
|
25.3
|
1.0
|
O
|
D:HOH693
|
2.6
|
29.7
|
1.0
|
C1
|
D:B12503
|
2.8
|
24.9
|
1.0
|
C19
|
D:B12503
|
2.8
|
26.7
|
1.0
|
C9
|
D:B12503
|
2.9
|
27.7
|
1.0
|
C4
|
D:B12503
|
2.9
|
30.3
|
1.0
|
C11
|
D:B12503
|
2.9
|
22.8
|
1.0
|
C14
|
D:B12503
|
2.9
|
24.1
|
1.0
|
C16
|
D:B12503
|
3.0
|
29.6
|
1.0
|
C6
|
D:B12503
|
3.0
|
29.5
|
1.0
|
C10
|
D:B12503
|
3.3
|
25.1
|
1.0
|
C5
|
D:B12503
|
3.3
|
27.0
|
1.0
|
C20
|
D:B12503
|
3.3
|
23.1
|
1.0
|
C15
|
D:B12503
|
3.4
|
27.1
|
1.0
|
C18
|
D:B12503
|
4.1
|
23.4
|
1.0
|
C2
|
D:B12503
|
4.1
|
24.6
|
1.0
|
C49
|
D:B12503
|
4.2
|
22.6
|
1.0
|
C8
|
D:B12503
|
4.2
|
25.8
|
1.0
|
C3
|
D:B12503
|
4.2
|
28.7
|
1.0
|
O
|
D:HOH695
|
4.2
|
31.5
|
1.0
|
C7
|
D:B12503
|
4.2
|
30.3
|
1.0
|
C13
|
D:B12503
|
4.2
|
26.1
|
1.0
|
C17
|
D:B12503
|
4.2
|
25.7
|
1.0
|
C12
|
D:B12503
|
4.3
|
28.1
|
1.0
|
O2
|
D:GOL504
|
4.4
|
27.4
|
1.0
|
O
|
D:HOH776
|
4.6
|
31.3
|
1.0
|
C26
|
D:B12503
|
4.7
|
31.2
|
1.0
|
C41
|
D:B12503
|
4.7
|
26.6
|
1.0
|
C35
|
D:B12503
|
4.8
|
27.9
|
1.0
|
C48
|
D:B12503
|
4.8
|
27.6
|
1.0
|
C37
|
D:B12503
|
4.8
|
30.0
|
1.0
|
C53
|
D:B12503
|
4.9
|
22.3
|
1.0
|
C46
|
D:B12503
|
4.9
|
28.3
|
1.0
|
NH2
|
D:ARG141
|
4.9
|
28.9
|
1.0
|
|
Reference:
D.P.Dowling,
Z.D.Miles,
C.Kohrer,
S.J.Maiocco,
S.J.Elliott,
V.Bandarian,
C.L.Drennan.
Molecular Basis of Cobalamin-Dependent Rna Modification. Nucleic Acids Res. V. 44 9965 2016.
ISSN: ESSN 1362-4962
PubMed: 27638883
DOI: 10.1093/NAR/GKW806
Page generated: Tue Jul 30 17:48:07 2024
|