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Cobalt in PDB 5d0a: Crystal Structure of Epoxyqueuosine Reductase with Cleaved Rna Stem Loop

Enzymatic activity of Crystal Structure of Epoxyqueuosine Reductase with Cleaved Rna Stem Loop

All present enzymatic activity of Crystal Structure of Epoxyqueuosine Reductase with Cleaved Rna Stem Loop:
1.17.99.6;

Protein crystallography data

The structure of Crystal Structure of Epoxyqueuosine Reductase with Cleaved Rna Stem Loop, PDB code: 5d0a was solved by D.P.Dowling, Z.D.Miles, C.Kohrer, V.Bandarian, C.L.Drennan, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 46.39 / 2.10
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 90.587, 111.118, 96.008, 90.00, 104.90, 90.00
R / Rfree (%) 18.4 / 22.5

Other elements in 5d0a:

The structure of Crystal Structure of Epoxyqueuosine Reductase with Cleaved Rna Stem Loop also contains other interesting chemical elements:

Iron (Fe) 32 atoms

Cobalt Binding Sites:

The binding sites of Cobalt atom in the Crystal Structure of Epoxyqueuosine Reductase with Cleaved Rna Stem Loop (pdb code 5d0a). This binding sites where shown within 5.0 Angstroms radius around Cobalt atom.
In total 4 binding sites of Cobalt where determined in the Crystal Structure of Epoxyqueuosine Reductase with Cleaved Rna Stem Loop, PDB code: 5d0a:
Jump to Cobalt binding site number: 1; 2; 3; 4;

Cobalt binding site 1 out of 4 in 5d0a

Go back to Cobalt Binding Sites List in 5d0a
Cobalt binding site 1 out of 4 in the Crystal Structure of Epoxyqueuosine Reductase with Cleaved Rna Stem Loop


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 1 of Crystal Structure of Epoxyqueuosine Reductase with Cleaved Rna Stem Loop within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Co503

b:25.4
occ:1.00
CO A:B12503 0.0 25.4 1.0
N21 A:B12503 1.9 24.3 1.0
N24 A:B12503 1.9 29.1 1.0
N22 A:B12503 1.9 28.2 1.0
N23 A:B12503 1.9 27.5 1.0
O A:HOH652 2.6 28.4 1.0
C1 A:B12503 2.8 22.6 1.0
C19 A:B12503 2.8 23.8 1.0
C9 A:B12503 2.9 30.2 1.0
C4 A:B12503 2.9 25.4 1.0
C11 A:B12503 2.9 27.7 1.0
C14 A:B12503 3.0 26.1 1.0
C6 A:B12503 3.0 26.4 1.0
C16 A:B12503 3.0 26.4 1.0
C20 A:B12503 3.3 24.9 1.0
C10 A:B12503 3.3 22.5 1.0
C5 A:B12503 3.3 27.8 1.0
C15 A:B12503 3.4 26.6 1.0
C18 A:B12503 4.1 22.0 1.0
C2 A:B12503 4.2 22.1 1.0
O A:HOH704 4.2 32.2 1.0
C8 A:B12503 4.2 25.7 1.0
C3 A:B12503 4.2 22.9 1.0
C7 A:B12503 4.2 28.6 1.0
C13 A:B12503 4.2 26.7 1.0
C17 A:B12503 4.3 22.6 1.0
C12 A:B12503 4.3 31.3 1.0
C49 A:B12503 4.3 26.8 1.0
O2 A:GOL504 4.4 25.2 1.0
O A:HOH770 4.5 31.5 1.0
C26 A:B12503 4.7 24.9 1.0
C35 A:B12503 4.8 22.8 1.0
C37 A:B12503 4.8 25.9 1.0
C48 A:B12503 4.8 27.8 1.0
C41 A:B12503 4.9 27.0 1.0
C53 A:B12503 4.9 23.9 1.0
C46 A:B12503 4.9 25.6 1.0
NH2 A:ARG141 4.9 26.4 1.0

Cobalt binding site 2 out of 4 in 5d0a

Go back to Cobalt Binding Sites List in 5d0a
Cobalt binding site 2 out of 4 in the Crystal Structure of Epoxyqueuosine Reductase with Cleaved Rna Stem Loop


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 2 of Crystal Structure of Epoxyqueuosine Reductase with Cleaved Rna Stem Loop within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Co503

b:27.3
occ:1.00
CO B:B12503 0.0 27.3 1.0
N21 B:B12503 1.9 29.7 1.0
N24 B:B12503 1.9 28.6 1.0
N23 B:B12503 1.9 27.5 1.0
N22 B:B12503 1.9 27.1 1.0
O B:HOH677 2.6 30.3 1.0
C1 B:B12503 2.8 27.9 1.0
C19 B:B12503 2.8 28.3 1.0
C9 B:B12503 2.9 30.7 1.0
C11 B:B12503 2.9 24.7 1.0
C4 B:B12503 2.9 30.3 1.0
C14 B:B12503 3.0 23.5 1.0
C16 B:B12503 3.0 25.2 1.0
C6 B:B12503 3.0 27.2 1.0
C10 B:B12503 3.3 22.5 1.0
C20 B:B12503 3.3 25.2 1.0
C5 B:B12503 3.3 29.2 1.0
C15 B:B12503 3.4 21.1 1.0
C18 B:B12503 4.1 26.3 1.0
O B:HOH696 4.1 29.2 1.0
C2 B:B12503 4.2 27.7 1.0
C3 B:B12503 4.2 26.2 1.0
C12 B:B12503 4.2 26.9 1.0
C8 B:B12503 4.2 26.0 1.0
C13 B:B12503 4.2 25.9 1.0
C7 B:B12503 4.2 27.5 1.0
C17 B:B12503 4.3 22.4 1.0
C49 B:B12503 4.3 25.8 1.0
O2 B:GOL504 4.4 29.5 1.0
O B:HOH783 4.7 28.2 1.0
C26 B:B12503 4.8 26.0 1.0
C35 B:B12503 4.8 27.6 1.0
C48 B:B12503 4.8 26.9 1.0
C37 B:B12503 4.8 22.4 1.0
C41 B:B12503 4.9 26.0 1.0
C46 B:B12503 4.9 28.1 1.0
NH2 B:ARG141 4.9 27.3 1.0
C53 B:B12503 4.9 26.2 1.0
C3 B:GOL504 4.9 30.7 1.0
C2 B:GOL504 5.0 29.8 1.0
C30 B:B12503 5.0 25.4 1.0

Cobalt binding site 3 out of 4 in 5d0a

Go back to Cobalt Binding Sites List in 5d0a
Cobalt binding site 3 out of 4 in the Crystal Structure of Epoxyqueuosine Reductase with Cleaved Rna Stem Loop


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 3 of Crystal Structure of Epoxyqueuosine Reductase with Cleaved Rna Stem Loop within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Co503

b:26.2
occ:1.00
CO C:B12503 0.0 26.2 1.0
N21 C:B12503 1.9 27.2 1.0
N24 C:B12503 1.9 28.3 1.0
N22 C:B12503 1.9 27.9 1.0
N23 C:B12503 1.9 24.4 1.0
O C:HOH678 2.5 27.9 1.0
C1 C:B12503 2.8 26.7 1.0
C19 C:B12503 2.8 24.9 1.0
C9 C:B12503 2.9 28.7 1.0
C4 C:B12503 2.9 24.5 1.0
C11 C:B12503 2.9 21.4 1.0
C14 C:B12503 2.9 25.7 1.0
C16 C:B12503 3.0 24.6 1.0
C6 C:B12503 3.0 28.6 1.0
C10 C:B12503 3.3 24.4 1.0
C20 C:B12503 3.3 22.8 1.0
C5 C:B12503 3.3 25.6 1.0
C15 C:B12503 3.4 26.9 1.0
C18 C:B12503 4.1 23.4 1.0
C2 C:B12503 4.1 27.5 1.0
O C:HOH700 4.2 29.5 1.0
C3 C:B12503 4.2 21.9 1.0
C49 C:B12503 4.2 24.0 1.0
C12 C:B12503 4.2 25.7 1.0
C8 C:B12503 4.2 27.7 1.0
C13 C:B12503 4.2 29.6 1.0
C17 C:B12503 4.3 25.3 1.0
C7 C:B12503 4.3 28.7 1.0
O2 C:GOL504 4.3 31.6 1.0
O C:HOH780 4.5 28.7 1.0
C26 C:B12503 4.7 25.5 1.0
C35 C:B12503 4.8 25.6 1.0
C41 C:B12503 4.8 26.6 1.0
C37 C:B12503 4.8 25.9 1.0
C46 C:B12503 4.8 22.5 1.0
C48 C:B12503 4.8 27.1 1.0
C53 C:B12503 4.9 21.1 1.0
C2 C:GOL504 4.9 29.4 1.0
C30 C:B12503 4.9 25.6 1.0
C3 C:GOL504 4.9 30.2 1.0
NH2 C:ARG141 5.0 26.2 1.0

Cobalt binding site 4 out of 4 in 5d0a

Go back to Cobalt Binding Sites List in 5d0a
Cobalt binding site 4 out of 4 in the Crystal Structure of Epoxyqueuosine Reductase with Cleaved Rna Stem Loop


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 4 of Crystal Structure of Epoxyqueuosine Reductase with Cleaved Rna Stem Loop within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Co503

b:26.1
occ:1.00
CO D:B12503 0.0 26.1 1.0
N21 D:B12503 1.9 26.6 1.0
N24 D:B12503 1.9 28.5 1.0
N22 D:B12503 1.9 27.5 1.0
N23 D:B12503 1.9 25.3 1.0
O D:HOH693 2.6 29.7 1.0
C1 D:B12503 2.8 24.9 1.0
C19 D:B12503 2.8 26.7 1.0
C9 D:B12503 2.9 27.7 1.0
C4 D:B12503 2.9 30.3 1.0
C11 D:B12503 2.9 22.8 1.0
C14 D:B12503 2.9 24.1 1.0
C16 D:B12503 3.0 29.6 1.0
C6 D:B12503 3.0 29.5 1.0
C10 D:B12503 3.3 25.1 1.0
C5 D:B12503 3.3 27.0 1.0
C20 D:B12503 3.3 23.1 1.0
C15 D:B12503 3.4 27.1 1.0
C18 D:B12503 4.1 23.4 1.0
C2 D:B12503 4.1 24.6 1.0
C49 D:B12503 4.2 22.6 1.0
C8 D:B12503 4.2 25.8 1.0
C3 D:B12503 4.2 28.7 1.0
O D:HOH695 4.2 31.5 1.0
C7 D:B12503 4.2 30.3 1.0
C13 D:B12503 4.2 26.1 1.0
C17 D:B12503 4.2 25.7 1.0
C12 D:B12503 4.3 28.1 1.0
O2 D:GOL504 4.4 27.4 1.0
O D:HOH776 4.6 31.3 1.0
C26 D:B12503 4.7 31.2 1.0
C41 D:B12503 4.7 26.6 1.0
C35 D:B12503 4.8 27.9 1.0
C48 D:B12503 4.8 27.6 1.0
C37 D:B12503 4.8 30.0 1.0
C53 D:B12503 4.9 22.3 1.0
C46 D:B12503 4.9 28.3 1.0
NH2 D:ARG141 4.9 28.9 1.0

Reference:

D.P.Dowling, Z.D.Miles, C.Kohrer, S.J.Maiocco, S.J.Elliott, V.Bandarian, C.L.Drennan. Molecular Basis of Cobalamin-Dependent Rna Modification. Nucleic Acids Res. V. 44 9965 2016.
ISSN: ESSN 1362-4962
PubMed: 27638883
DOI: 10.1093/NAR/GKW806
Page generated: Tue Jul 30 17:48:07 2024

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