Cobalt in PDB 5uxm: Type II DAH7PS From Pseudomonas Aeruginosa with Trp Bound

All present enzymatic activity of Type II DAH7PS From Pseudomonas Aeruginosa with Trp Bound:
2.5.1.54;

The structure of Type II DAH7PS From Pseudomonas Aeruginosa with Trp Bound, PDB code: 5uxm was solved by O.W.Sterritt, G.B.Jameson, E.J.Parker, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	75.79 / 1.54
Space group	I 2 2 2
Cell size a, b, c (Å), α, β, γ (°)	81.680, 100.370, 115.600, 90.00, 90.00, 90.00
R / Rfree (%)	18.5 / 21

The structure of Type II DAH7PS From Pseudomonas Aeruginosa with Trp Bound also contains other interesting chemical elements:

Chlorine

(Cl)

2 atoms

The binding sites of Cobalt atom in the Type II DAH7PS From Pseudomonas Aeruginosa with Trp Bound (pdb code 5uxm). This binding sites where shown within 5.0 Angstroms radius around Cobalt atom.
In total only one binding site of Cobalt was determined in the Type II DAH7PS From Pseudomonas Aeruginosa with Trp Bound, PDB code: 5uxm:

Cobalt binding site 1 out of 1 in the Type II DAH7PS From Pseudomonas Aeruginosa with Trp Bound


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 1 of Type II DAH7PS From Pseudomonas Aeruginosa with Trp Bound within 5.0Å range: probe atom residue distance (Å) B Occ A:Co505 b:21.3 occ:0.75 O A:HOH739 1.8 27.2 0.8 OE1 A:GLU394 2.1 16.9 1.0 OD2 A:ASP424 2.3 33.4 1.0 NE2 A:HIS352 2.3 19.6 1.0 OE2 A:GLU394 2.4 18.9 1.0 CD A:GLU394 2.5 16.0 1.0 SG A:CYS69 2.6 18.9 0.8 CE1 A:HIS352 3.3 18.2 1.0 CD2 A:HIS352 3.3 18.3 1.0 CG A:ASP424 3.3 31.7 1.0 SG A:CYS69 3.5 13.5 0.2 CB A:ASP424 3.7 26.5 1.0 NH2 A:ARG365 3.7 25.6 1.0 CB A:CYS69 3.8 16.0 0.8 O2' A:PEP501 3.9 18.5 1.0 CB A:CYS69 4.0 13.4 0.2 CG A:GLU394 4.0 14.1 1.0 O A:HOH847 4.1 23.0 1.0 CA A:CYS69 4.2 15.2 0.8 C1 A:PEP501 4.3 17.7 1.0 ND1 A:HIS352 4.4 17.0 1.0 CA A:CYS69 4.4 13.3 0.2 OD1 A:ASP424 4.4 36.3 1.0 CG A:HIS352 4.4 17.5 1.0 NH1 A:ARG365 4.4 22.5 1.0 CZ A:ARG365 4.6 23.8 1.0 N A:ASP424 4.6 19.7 1.0 NH2 A:ARG108 4.6 13.6 1.0 C2 A:PEP501 4.7 18.0 1.0 CA A:ASP424 4.7 22.1 1.0 O A:CYS69 4.9 14.7 0.8 C3 A:PEP501 4.9 18.3 1.0 O A:CYS69 4.9 13.8 0.2 O1 A:PEP501 4.9 16.6 1.0 C A:CYS69 5.0 14.4 0.8

O.W.Sterritt, S.A.Kessans, G.B.Jameson, E.J.Parker. A Pseudoisostructural Type II DAH7PS Enzyme From Pseudomonas Aeruginosa: Alternative Evolutionary Strategies to Control Shikimate Pathway Flux. Biochemistry V. 57 2667 2018.
ISSN: ISSN 1520-4995
PubMed: 29608284
DOI: 10.1021/ACS.BIOCHEM.8B00082