Cobalt in PDB 6bmc: The Structure of A Dimeric Type II DAH7PS Associated with Pyocyanin Biosynthesis in Pseudomonas Aeruginosa

All present enzymatic activity of The Structure of A Dimeric Type II DAH7PS Associated with Pyocyanin Biosynthesis in Pseudomonas Aeruginosa:
2.5.1.54;

The structure of The Structure of A Dimeric Type II DAH7PS Associated with Pyocyanin Biosynthesis in Pseudomonas Aeruginosa, PDB code: 6bmc was solved by O.W.Sterritt, G.B.Jameson, E.J.Parker, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	85.27 / 2.70
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	53.757, 169.722, 170.550, 90.00, 90.00, 90.00
R / Rfree (%)	24.8 / 29

The structure of The Structure of A Dimeric Type II DAH7PS Associated with Pyocyanin Biosynthesis in Pseudomonas Aeruginosa also contains other interesting chemical elements:

Chlorine

(Cl)

1 atom

The binding sites of Cobalt atom in the The Structure of A Dimeric Type II DAH7PS Associated with Pyocyanin Biosynthesis in Pseudomonas Aeruginosa (pdb code 6bmc). This binding sites where shown within 5.0 Angstroms radius around Cobalt atom.
In total 2 binding sites of Cobalt where determined in the The Structure of A Dimeric Type II DAH7PS Associated with Pyocyanin Biosynthesis in Pseudomonas Aeruginosa, PDB code: 6bmc:
Jump to Cobalt binding site number: 1; 2;

Cobalt binding site 1 out of 2 in the The Structure of A Dimeric Type II DAH7PS Associated with Pyocyanin Biosynthesis in Pseudomonas Aeruginosa


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 1 of The Structure of A Dimeric Type II DAH7PS Associated with Pyocyanin Biosynthesis in Pseudomonas Aeruginosa within 5.0Å range: probe atom residue distance (Å) B Occ A:Co502 b:83.2 occ:1.00 O A:HOH601 1.8 61.6 1.0 SG A:CYS67 2.1 77.6 1.0 OE1 A:GLU345 2.2 91.5 1.0 OD2 A:ASP373 2.4 92.8 1.0 NE2 A:HIS303 2.4 69.9 1.0 OE2 A:GLU345 2.7 85.8 1.0 CD A:GLU345 2.8 84.8 1.0 CG A:ASP373 3.2 87.8 1.0 CE1 A:HIS303 3.2 71.1 1.0 CB A:CYS67 3.2 76.5 1.0 CB A:ASP373 3.4 85.4 1.0 CD2 A:HIS303 3.4 70.6 1.0 NZ A:LYS113 3.4 81.3 1.0 O2' A:PEP501 3.4 67.1 1.0 CA A:CYS67 3.8 73.7 1.0 C1 A:PEP501 4.1 70.6 1.0 CG A:GLU345 4.2 80.0 1.0 OD1 A:ASP373 4.3 86.9 1.0 NH2 A:ARG316 4.3 98.8 1.0 ND1 A:HIS303 4.3 71.9 1.0 CE A:LYS113 4.4 79.3 1.0 CG A:HIS303 4.5 70.5 1.0 NH1 A:ARG106 4.6 81.9 1.0 C2 A:PEP501 4.6 68.7 1.0 CA A:ASP373 4.7 84.0 1.0 O1 A:PEP501 4.7 74.5 1.0 C A:CYS67 4.7 71.3 1.0 NH2 A:ARG106 4.8 86.9 1.0 N A:ASP373 4.8 83.4 1.0 O A:HOH609 4.8 55.2 1.0 NH1 A:ARG316 4.8 97.8 1.0 N A:CYS67 4.9 75.6 1.0 O A:CYS67 4.9 69.7 1.0

Cobalt binding site 2 out of 2 in the The Structure of A Dimeric Type II DAH7PS Associated with Pyocyanin Biosynthesis in Pseudomonas Aeruginosa


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 2 of The Structure of A Dimeric Type II DAH7PS Associated with Pyocyanin Biosynthesis in Pseudomonas Aeruginosa within 5.0Å range: probe atom residue distance (Å) B Occ B:Co501 b:88.6 occ:1.00 OE2 B:GLU345 1.9 81.1 1.0 O B:HOH602 2.1 93.3 1.0 NE2 B:HIS303 2.1 92.2 1.0 SG B:CYS67 2.3 82.5 1.0 CD B:GLU345 2.5 78.7 1.0 OE1 B:GLU345 2.6 85.0 1.0 OD2 B:ASP373 2.8 93.0 1.0 CD2 B:HIS303 2.8 92.8 1.0 CE1 B:HIS303 3.3 90.5 1.0 NZ B:LYS113 3.4 94.6 1.0 CB B:CYS67 3.5 80.5 1.0 CG B:ASP373 3.6 94.1 1.0 CB B:ASP373 3.6 93.1 1.0 O2' B:PEP502 3.8 90.9 1.0 CG B:GLU345 3.9 75.2 1.0 CA B:CYS67 4.0 79.5 1.0 CG B:HIS303 4.0 92.4 1.0 ND1 B:HIS303 4.2 90.4 1.0 NH2 B:ARG316 4.2 98.6 1.0 C1 B:PEP502 4.3 96.1 1.0 NH2 B:ARG106 4.5 85.7 1.0 O1 B:PEP502 4.6 0.3 1.0 OD1 B:ASP373 4.7 94.2 1.0 CE B:LYS113 4.8 93.4 1.0 NH1 B:ARG316 4.8 99.0 1.0 NH1 B:ARG106 4.9 86.9 1.0 CA B:ASP373 4.9 94.8 1.0 C B:CYS67 4.9 79.3 1.0 CB B:GLU345 4.9 74.6 1.0 O B:CYS67 5.0 76.4 1.0 CD B:LYS113 5.0 95.0 1.0 CZ B:ARG106 5.0 84.9 1.0

O.W.Sterritt, E.J.M.Lang, S.A.Kessans, T.M.Ryan, B.Demeler, G.B.Jameson, E.J.Parker. Structural and Functional Characterisation of the Entry Point to Pyocyanin Biosynthesis Inpseudomonas Aeruginosadefines A New 3-Deoxy-D-Arabino-Heptulosonate 7-Phosphate Synthase Subclass. Biosci. Rep. V. 38 2018.
ISSN: ISSN 1573-4935
PubMed: 30242059
DOI: 10.1042/BSR20181605