Cobalt in PDB 6ec0: Crystal Structure of the Wild-Type Heterocomplex Between Coil 1B Domains of Human Intermediate Filament Proteins Keratin 1 (KRT1) and Keratin 10 (KRT10)

The structure of Crystal Structure of the Wild-Type Heterocomplex Between Coil 1B Domains of Human Intermediate Filament Proteins Keratin 1 (KRT1) and Keratin 10 (KRT10), PDB code: 6ec0 was solved by S.A.Eldirany, I.B.Lomakin, C.G.Bunick, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	46.20 / 2.98
Space group	P 31 2 1
Cell size a, b, c (Å), α, β, γ (°)	106.685, 106.685, 70.321, 90.00, 90.00, 120.00
R / Rfree (%)	28.1 / 30

The structure of Crystal Structure of the Wild-Type Heterocomplex Between Coil 1B Domains of Human Intermediate Filament Proteins Keratin 1 (KRT1) and Keratin 10 (KRT10) also contains other interesting chemical elements:

Nickel	(Ni)	2 atoms
Cadmium	(Cd)	4 atoms

The binding sites of Cobalt atom in the Crystal Structure of the Wild-Type Heterocomplex Between Coil 1B Domains of Human Intermediate Filament Proteins Keratin 1 (KRT1) and Keratin 10 (KRT10) (pdb code 6ec0). This binding sites where shown within 5.0 Angstroms radius around Cobalt atom.
In total 2 binding sites of Cobalt where determined in the Crystal Structure of the Wild-Type Heterocomplex Between Coil 1B Domains of Human Intermediate Filament Proteins Keratin 1 (KRT1) and Keratin 10 (KRT10), PDB code: 6ec0:
Jump to Cobalt binding site number: 1; 2;

Cobalt binding site 1 out of 2 in the Crystal Structure of the Wild-Type Heterocomplex Between Coil 1B Domains of Human Intermediate Filament Proteins Keratin 1 (KRT1) and Keratin 10 (KRT10)


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 1 of Crystal Structure of the Wild-Type Heterocomplex Between Coil 1B Domains of Human Intermediate Filament Proteins Keratin 1 (KRT1) and Keratin 10 (KRT10) within 5.0Å range: probe atom residue distance (Å) B Occ A:Co404 b:0.8 occ:1.00 OD2 A:ASP253 2.8 0.2 1.0 HE21 A:GLN249 3.1 0.6 1.0 CG A:ASP253 3.5 0.2 1.0 OD1 A:ASP253 3.7 0.1 1.0 NE2 A:GLN249 3.7 98.8 1.0 HE22 A:GLN249 4.0 0.6 1.0 O A:HOH514 4.2 40.7 1.0 HG2 A:GLN249 4.3 0.3 1.0 HB3 A:ASP253 4.5 0.1 1.0 HG3 A:GLN249 4.6 0.3 1.0 CD A:GLN249 4.6 97.5 1.0 CB A:ASP253 4.6 0.8 1.0 CG A:GLN249 4.7 0.4 1.0 HD11 B:ILE220 4.9 85.1 1.0 HB2 A:ASP253 5.0 0.1 1.0

Cobalt binding site 2 out of 2 in the Crystal Structure of the Wild-Type Heterocomplex Between Coil 1B Domains of Human Intermediate Filament Proteins Keratin 1 (KRT1) and Keratin 10 (KRT10)


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 2 of Crystal Structure of the Wild-Type Heterocomplex Between Coil 1B Domains of Human Intermediate Filament Proteins Keratin 1 (KRT1) and Keratin 10 (KRT10) within 5.0Å range: probe atom residue distance (Å) B Occ B:Co302 b:0.7 occ:1.00 HB3 B:ASN212 3.1 0.1 1.0 OD2 B:ASP216 3.3 90.7 1.0 O B:HOH417 3.3 0.5 1.0 OD1 B:ASP216 3.3 0.9 1.0 CG B:ASP216 3.7 88.8 1.0 CB B:ASN212 3.9 0.1 1.0 O B:HOH402 4.0 59.5 1.0 HD22 B:ASN212 4.2 0.9 1.0 CG B:ASN212 4.2 0.9 1.0 ND2 B:ASN212 4.3 0.4 1.0 HA B:ASN212 4.3 0.3 1.0 O B:ASN212 4.3 79.1 1.0 CA B:ASN212 4.6 0.6 1.0 HB2 B:ASN212 4.7 0.1 1.0 C B:ASN212 4.7 94.0 1.0 HD21 B:ASN212 4.7 0.9 1.0 OD1 B:ASN212 4.8 93.1 1.0

S.A.Eldirany, M.Ho, A.J.Hinbest, I.B.Lomakin, C.G.Bunick. Human Keratin 1/10-1B Tetramer Structures Reveal A Knob-Pocket Mechanism in Intermediate Filament Assembly. Embo J. V. 38 2019.
ISSN: ESSN 1460-2075
PubMed: 31036554
DOI: 10.15252/EMBJ.2018100741