Cobalt in PDB 6h9e: Structure of Glutamate Mutase Reconstituted with Homo-Coenzyme B12

All present enzymatic activity of Structure of Glutamate Mutase Reconstituted with Homo-Coenzyme B12:
5.4.99.1;

The structure of Structure of Glutamate Mutase Reconstituted with Homo-Coenzyme B12, PDB code: 6h9e was solved by K.Gruber, V.Csitkovits, C.Kratky, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	40.41 / 1.82
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	63.890, 112.620, 108.350, 90.00, 95.69, 90.00
R / Rfree (%)	13.7 / 16.8

The binding sites of Cobalt atom in the Structure of Glutamate Mutase Reconstituted with Homo-Coenzyme B12 (pdb code 6h9e). This binding sites where shown within 5.0 Angstroms radius around Cobalt atom.
In total 2 binding sites of Cobalt where determined in the Structure of Glutamate Mutase Reconstituted with Homo-Coenzyme B12, PDB code: 6h9e:
Jump to Cobalt binding site number: 1; 2;

Cobalt binding site 1 out of 2 in the Structure of Glutamate Mutase Reconstituted with Homo-Coenzyme B12


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 1 of Structure of Glutamate Mutase Reconstituted with Homo-Coenzyme B12 within 5.0Å range: probe atom residue distance (Å) B Occ A:Co201 b:7.3 occ:1.00 CO A:B12201 0.0 7.3 1.0 N21 A:B12201 1.9 6.8 1.0 N24 A:B12201 1.9 6.3 1.0 N23 A:B12201 1.9 7.9 1.0 N22 A:B12201 1.9 5.3 1.0 C6' B:FWK501 2.1 6.8 1.0 NE2 A:HIS16 2.2 9.0 1.0 C19 A:B12201 2.8 6.1 1.0 C1 A:B12201 2.9 7.8 1.0 C9 A:B12201 2.9 5.7 1.0 C11 A:B12201 2.9 7.3 1.0 C4 A:B12201 2.9 9.0 1.0 C14 A:B12201 3.0 4.5 1.0 C16 A:B12201 3.0 4.5 1.0 C6 A:B12201 3.0 5.1 1.0 CE1 A:HIS16 3.1 8.4 1.0 C5' B:FWK501 3.1 9.5 1.0 C10 A:B12201 3.2 5.8 1.0 CD2 A:HIS16 3.3 6.6 1.0 C5 A:B12201 3.4 7.5 1.0 C15 A:B12201 3.4 5.4 1.0 C20 A:B12201 3.5 6.8 1.0 C18 A:B12201 4.1 4.6 1.0 C2 A:B12201 4.1 8.9 1.0 C8 A:B12201 4.2 6.2 1.0 C12 A:B12201 4.2 6.7 1.0 C3 A:B12201 4.2 7.5 1.0 C13 A:B12201 4.2 6.4 1.0 C17 A:B12201 4.2 5.6 1.0 ND1 A:HIS16 4.3 8.6 1.0 C7 A:B12201 4.3 5.8 1.0 CG A:HIS16 4.4 6.8 1.0 C4' B:FWK501 4.5 10.3 1.0 C26 A:B12201 4.6 6.2 1.0 C46 A:B12201 4.7 7.4 1.0 C48 A:B12201 4.8 7.6 1.0 O B:HOH776 4.8 5.7 1.0 C3' B:FWK501 4.9 9.6 1.0 C35 A:B12201 4.9 7.4 1.0 C41 A:B12201 4.9 5.1 1.0 C53 A:B12201 4.9 6.2 1.0 C54 A:B12201 4.9 5.2 1.0

Cobalt binding site 2 out of 2 in the Structure of Glutamate Mutase Reconstituted with Homo-Coenzyme B12


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 2 of Structure of Glutamate Mutase Reconstituted with Homo-Coenzyme B12 within 5.0Å range: probe atom residue distance (Å) B Occ C:Co201 b:8.0 occ:1.00 CO C:B12201 0.0 8.0 1.0 N21 C:B12201 1.9 6.3 1.0 N24 C:B12201 1.9 7.1 1.0 N23 C:B12201 1.9 7.7 1.0 N22 C:B12201 1.9 7.1 1.0 C6' D:FWK501 2.1 6.7 1.0 NE2 C:HIS16 2.2 7.0 1.0 C19 C:B12201 2.8 6.8 1.0 C11 C:B12201 2.9 4.9 1.0 C1 C:B12201 2.9 7.0 1.0 C9 C:B12201 2.9 8.0 1.0 C4 C:B12201 2.9 7.2 1.0 C14 C:B12201 2.9 5.8 1.0 C16 C:B12201 3.0 5.8 1.0 C6 C:B12201 3.0 7.0 1.0 CE1 C:HIS16 3.0 9.1 1.0 C5' D:FWK501 3.1 8.5 1.0 C10 C:B12201 3.2 5.1 1.0 CD2 C:HIS16 3.3 6.3 1.0 C15 C:B12201 3.4 6.8 1.0 C5 C:B12201 3.4 7.5 1.0 C20 C:B12201 3.6 7.4 1.0 C18 C:B12201 4.1 4.5 1.0 C2 C:B12201 4.2 8.6 1.0 C12 C:B12201 4.2 5.8 1.0 C13 C:B12201 4.2 6.5 1.0 C3 C:B12201 4.2 6.5 1.0 C8 C:B12201 4.2 6.3 1.0 ND1 C:HIS16 4.2 7.3 1.0 C17 C:B12201 4.2 5.6 1.0 C7 C:B12201 4.3 6.0 1.0 CG C:HIS16 4.4 5.7 1.0 C4' D:FWK501 4.5 9.1 1.0 C26 C:B12201 4.6 7.2 1.0 C48 C:B12201 4.7 7.2 1.0 C46 C:B12201 4.7 6.8 1.0 O D:HOH796 4.8 7.7 1.0 C53 C:B12201 4.9 6.9 1.0 C3' D:FWK501 4.9 9.4 1.0 C35 C:B12201 4.9 7.3 1.0 C54 C:B12201 4.9 6.1 1.0 C41 C:B12201 5.0 8.3 1.0

C.Csitkovits, A.Lyskowski, S.Gschoesser, C.Kratky, B.Kraeutler, K.Gruber. Structures of Glutamate Mutase Reconstituted with Homo- and Bishomo-Coenzyme B12 To Be Published.