Cobalt in PDB 6h9f: Structure of Glutamate Mutase Reconstituted with Bishomo-Coenzyme B12

All present enzymatic activity of Structure of Glutamate Mutase Reconstituted with Bishomo-Coenzyme B12:
5.4.99.1;

The structure of Structure of Glutamate Mutase Reconstituted with Bishomo-Coenzyme B12, PDB code: 6h9f was solved by K.Gruber, V.Csitkovits, C.Kratky, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	35.35 / 2.10
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	63.749, 112.286, 108.010, 90.00, 95.71, 90.00
R / Rfree (%)	15.8 / 20.3

The binding sites of Cobalt atom in the Structure of Glutamate Mutase Reconstituted with Bishomo-Coenzyme B12 (pdb code 6h9f). This binding sites where shown within 5.0 Angstroms radius around Cobalt atom.
In total 2 binding sites of Cobalt where determined in the Structure of Glutamate Mutase Reconstituted with Bishomo-Coenzyme B12, PDB code: 6h9f:
Jump to Cobalt binding site number: 1; 2;

Cobalt binding site 1 out of 2 in the Structure of Glutamate Mutase Reconstituted with Bishomo-Coenzyme B12


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 1 of Structure of Glutamate Mutase Reconstituted with Bishomo-Coenzyme B12 within 5.0Å range: probe atom residue distance (Å) B Occ A:Co201 b:6.8 occ:1.00 CO A:B12201 0.0 6.8 1.0 N21 A:B12201 1.9 7.5 1.0 N24 A:B12201 1.9 5.8 1.0 N23 A:B12201 1.9 5.5 1.0 N22 A:B12201 1.9 7.2 1.0 C24 B:8ZB501 2.0 8.6 1.0 NE2 A:HIS16 2.2 8.4 1.0 C19 A:B12201 2.8 5.9 1.0 C28 B:8ZB501 2.8 8.0 1.0 C1 A:B12201 2.9 9.3 1.0 C9 A:B12201 2.9 5.4 1.0 C11 A:B12201 2.9 6.2 1.0 C4 A:B12201 2.9 6.8 1.0 C14 A:B12201 3.0 5.1 1.0 C16 A:B12201 3.0 4.5 1.0 C6 A:B12201 3.0 5.8 1.0 CE1 A:HIS16 3.1 6.9 1.0 C10 A:B12201 3.2 5.4 1.0 CD2 A:HIS16 3.3 8.9 1.0 C5 A:B12201 3.4 5.5 1.0 C15 A:B12201 3.4 5.4 1.0 C20 A:B12201 3.6 6.2 1.0 C18 A:B12201 4.1 8.8 1.0 C2 A:B12201 4.1 6.0 1.0 C8 A:B12201 4.2 5.2 1.0 C3 A:B12201 4.2 6.5 1.0 C12 A:B12201 4.2 8.3 1.0 C31 B:8ZB501 4.2 8.7 1.0 C13 A:B12201 4.2 9.0 1.0 ND1 A:HIS16 4.2 7.0 1.0 C17 A:B12201 4.2 5.7 1.0 C7 A:B12201 4.2 7.3 1.0 CG A:HIS16 4.4 7.1 1.0 C26 A:B12201 4.5 6.0 1.0 O B:HOH719 4.7 14.4 1.0 C46 A:B12201 4.7 5.6 1.0 C48 A:B12201 4.7 7.4 1.0 C35 A:B12201 4.9 7.3 1.0 C54 A:B12201 4.9 7.2 1.0 C53 A:B12201 4.9 5.0 1.0 C41 A:B12201 4.9 5.6 1.0

Cobalt binding site 2 out of 2 in the Structure of Glutamate Mutase Reconstituted with Bishomo-Coenzyme B12


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 2 of Structure of Glutamate Mutase Reconstituted with Bishomo-Coenzyme B12 within 5.0Å range: probe atom residue distance (Å) B Occ C:Co201 b:6.5 occ:1.00 CO C:B12201 0.0 6.5 1.0 N21 C:B12201 1.9 5.6 1.0 N24 C:B12201 1.9 4.1 1.0 N23 C:B12201 1.9 4.8 1.0 N22 C:B12201 1.9 5.8 1.0 C24 D:8ZB501 2.0 6.3 1.0 NE2 C:HIS16 2.2 3.8 1.0 C19 C:B12201 2.8 8.4 1.0 C11 C:B12201 2.9 4.0 1.0 C1 C:B12201 2.9 6.9 1.0 C9 C:B12201 2.9 5.6 1.0 CE1 C:HIS16 2.9 7.3 1.0 C4 C:B12201 2.9 6.7 1.0 C14 C:B12201 3.0 5.1 1.0 C16 C:B12201 3.0 5.3 1.0 C6 C:B12201 3.0 6.6 1.0 C28 D:8ZB501 3.1 8.7 1.0 C10 C:B12201 3.2 3.9 1.0 CD2 C:HIS16 3.3 6.3 1.0 C5 C:B12201 3.4 5.9 1.0 C15 C:B12201 3.4 5.7 1.0 C20 C:B12201 3.5 4.2 1.0 ND1 C:HIS16 4.1 6.2 1.0 C18 C:B12201 4.2 4.7 1.0 C2 C:B12201 4.2 6.2 1.0 C12 C:B12201 4.2 5.6 1.0 C13 C:B12201 4.2 5.1 1.0 C3 C:B12201 4.2 6.5 1.0 C8 C:B12201 4.2 6.5 1.0 C17 C:B12201 4.2 7.1 1.0 C7 C:B12201 4.3 7.2 1.0 C31 D:8ZB501 4.3 11.1 1.0 CG C:HIS16 4.3 5.1 1.0 C26 C:B12201 4.6 6.6 1.0 C46 C:B12201 4.7 3.6 1.0 C48 C:B12201 4.7 4.5 1.0 C35 C:B12201 4.9 6.3 1.0 C54 C:B12201 4.9 5.6 1.0 C53 C:B12201 4.9 3.9 1.0 O D:HOH731 4.9 11.4 1.0 C41 C:B12201 5.0 5.2 1.0

C.Csitkovits, A.Lyskowski, S.Gschoesser, C.Kratky, B.Kraeutler, K.Gruber. Structures of Glutamate Mutase Reconstituted with Homo- and Bishomo-Coenzyme B12 To Be Published.