Cobalt in PDB 6i5j: Crystal Structure of SOCS2:Elongin C:Elongin B in Complex with Growth Hormone Receptor Peptide

The structure of Crystal Structure of SOCS2:Elongin C:Elongin B in Complex with Growth Hormone Receptor Peptide, PDB code: 6i5j was solved by W.W.Kung, S.Ramachandran, N.Makukhin, E.Bruno, A.Ciulli, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	64.59 / 2.80
Space group	P 2 21 21
Cell size a, b, c (Å), α, β, γ (°)	57.835, 113.707, 156.944, 90.00, 90.00, 90.00
R / Rfree (%)	21 / 26.4

The structure of Crystal Structure of SOCS2:Elongin C:Elongin B in Complex with Growth Hormone Receptor Peptide also contains other interesting chemical elements:

Arsenic

(As)

5 atoms

The binding sites of Cobalt atom in the Crystal Structure of SOCS2:Elongin C:Elongin B in Complex with Growth Hormone Receptor Peptide (pdb code 6i5j). This binding sites where shown within 5.0 Angstroms radius around Cobalt atom.
In total 2 binding sites of Cobalt where determined in the Crystal Structure of SOCS2:Elongin C:Elongin B in Complex with Growth Hormone Receptor Peptide, PDB code: 6i5j:
Jump to Cobalt binding site number: 1; 2;

Cobalt binding site 1 out of 2 in the Crystal Structure of SOCS2:Elongin C:Elongin B in Complex with Growth Hormone Receptor Peptide


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 1 of Crystal Structure of SOCS2:Elongin C:Elongin B in Complex with Growth Hormone Receptor Peptide within 5.0Å range: probe atom residue distance (Å) B Occ A:Co201 b:52.9 occ:1.00 NE2 A:HIS149 2.1 49.6 1.0 O A:HOH301 2.2 54.2 1.0 O K:HOH103 2.2 67.7 1.0 NE2 K:HIS4 2.3 44.0 1.0 CE1 A:HIS149 2.7 51.5 1.0 CE1 K:HIS4 3.1 43.1 1.0 CD2 A:HIS149 3.3 50.2 1.0 CD2 K:HIS4 3.5 45.5 1.0 ND1 A:HIS149 3.9 49.9 1.0 CG A:HIS149 4.2 47.7 1.0 O A:THR147 4.2 59.0 1.0 ND1 K:HIS4 4.3 41.7 1.0 CG K:HIS4 4.5 42.8 1.0

Cobalt binding site 2 out of 2 in the Crystal Structure of SOCS2:Elongin C:Elongin B in Complex with Growth Hormone Receptor Peptide


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 2 of Crystal Structure of SOCS2:Elongin C:Elongin B in Complex with Growth Hormone Receptor Peptide within 5.0Å range: probe atom residue distance (Å) B Occ D:Co201 b:89.9 occ:1.00 CE1 D:HIS149 2.2 87.6 1.0 NE2 D:HIS149 2.2 86.3 1.0 NE2 L:HIS4 2.4 92.1 1.0 CD2 L:HIS4 3.1 91.4 1.0 CE1 L:HIS4 3.3 91.9 1.0 ND1 D:HIS149 3.4 84.6 1.0 CD2 D:HIS149 3.5 85.7 1.0 CG D:HIS149 4.0 81.5 1.0 CG L:HIS4 4.2 90.0 1.0 ND1 L:HIS4 4.3 91.5 1.0 O D:THR147 4.3 70.5 1.0 CB D:THR147 4.9 74.8 1.0

W.W.Kung, S.Ramachandran, N.Makukhin, E.Bruno, A.Ciulli. Structural Insights Into Substrate Recognition By the SOCS2 E3 Ubiquitin Ligase. Nat Commun V. 10 2534 2019.
ISSN: ESSN 2041-1723
PubMed: 31182716
DOI: 10.1038/S41467-019-10190-4