Cobalt in PDB 6iu8: Crystal Structure of Cytoplasmic Metal Binding Domain with Cobalt Ions

The structure of Crystal Structure of Cytoplasmic Metal Binding Domain with Cobalt Ions, PDB code: 6iu8 was solved by T.Kato, T.Nishizawa, K.Yamashita, K.Kumazaki, R.Ishitani, O.Nureki, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.72 / 2.70
Space group	P 31
Cell size a, b, c (Å), α, β, γ (°)	85.503, 85.503, 98.355, 90.00, 90.00, 120.00
R / Rfree (%)	15.8 / 21.5

The structure of Crystal Structure of Cytoplasmic Metal Binding Domain with Cobalt Ions also contains other interesting chemical elements:

Zinc

(Zn)

15 atoms

Pages:

>>> Page 1 <<< Page 2, Binding sites: 11 - 18;

Binding sites:

The binding sites of Cobalt atom in the Crystal Structure of Cytoplasmic Metal Binding Domain with Cobalt Ions (pdb code 6iu8). This binding sites where shown within 5.0 Angstroms radius around Cobalt atom.
In total 18 binding sites of Cobalt where determined in the Crystal Structure of Cytoplasmic Metal Binding Domain with Cobalt Ions, PDB code: 6iu8:
Jump to Cobalt binding site number: 1; 2; 3; 4; 5; 6; 7; 8; 9; 10;

Cobalt binding site 1 out of 18 in the Crystal Structure of Cytoplasmic Metal Binding Domain with Cobalt Ions


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 1 of Crystal Structure of Cytoplasmic Metal Binding Domain with Cobalt Ions within 5.0Å range: probe atom residue distance (Å) B Occ A:Co202 b:78.3 occ:1.00 OE2 A:GLU113 1.5 68.1 1.0 OE1 A:GLU102 1.8 63.5 1.0 O A:HOH301 1.9 44.1 1.0 OE1 A:GLU116 2.1 57.4 1.0 OE2 A:GLU153 2.2 62.0 1.0 CD A:GLU113 2.6 57.0 1.0 SD A:MET149 2.6 66.9 1.0 CD A:GLU102 3.0 59.2 1.0 OE1 A:GLU113 3.1 47.6 1.0 CD A:GLU153 3.3 65.6 1.0 CD A:GLU116 3.3 69.0 1.0 OE2 A:GLU102 3.5 42.0 1.0 ZN A:ZN203 3.6 74.0 1.0 ZN A:ZN201 3.7 76.4 1.0 CG A:MET149 3.8 87.6 1.0 CG A:GLU113 3.9 55.8 1.0 OE1 A:GLU153 3.9 64.8 1.0 OE2 A:GLU116 3.9 58.6 1.0 CE A:MET149 3.9 70.8 1.0 CB A:MET149 4.0 80.1 1.0 CG A:GLU102 4.2 63.1 1.0 OE2 A:GLU105 4.3 97.5 1.0 CG A:GLU153 4.4 57.4 1.0 CB A:GLU113 4.5 56.3 1.0 CG A:GLU116 4.5 70.0 1.0 CB A:GLU116 4.5 70.3 1.0 CA A:GLU113 4.8 55.6 1.0 CB A:GLU153 4.8 64.0 1.0

Cobalt binding site 2 out of 18 in the Crystal Structure of Cytoplasmic Metal Binding Domain with Cobalt Ions


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 2 of Crystal Structure of Cytoplasmic Metal Binding Domain with Cobalt Ions within 5.0Å range: probe atom residue distance (Å) B Occ A:Co204 b:93.2 occ:1.00 NE2 A:HIS129 2.1 80.2 1.0 CE1 A:HIS129 2.6 70.6 1.0 CD2 A:HIS129 3.5 76.7 1.0 OE2 A:GLU127 3.6 93.3 1.0 OE1 A:GLU130 3.7 64.2 1.0 ND1 A:HIS129 3.8 72.4 1.0 CD A:GLU127 4.0 97.6 1.0 CD A:GLU130 4.2 67.3 1.0 CG A:HIS129 4.3 80.7 1.0 CG A:GLU127 4.3 96.4 1.0 OE1 A:GLU127 4.7 1.0 1.0 OE2 A:GLU130 4.8 73.7 1.0 CG A:GLU130 4.9 62.3 1.0 CB A:GLU127 5.0 89.3 1.0

Cobalt binding site 3 out of 18 in the Crystal Structure of Cytoplasmic Metal Binding Domain with Cobalt Ions


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 3 of Crystal Structure of Cytoplasmic Metal Binding Domain with Cobalt Ions within 5.0Å range: probe atom residue distance (Å) B Occ B:Co202 b:49.0 occ:1.00 OE1 B:GLU113 1.9 53.4 1.0 OE1 B:GLU153 1.9 51.2 1.0 OE2 B:GLU102 1.9 41.6 1.0 OE2 B:GLU116 1.9 52.4 1.0 O B:HOH301 2.5 40.9 1.0 CD B:GLU113 2.7 58.0 1.0 CD B:GLU153 2.7 63.0 1.0 OE2 B:GLU113 2.8 46.0 1.0 CD B:GLU102 2.9 53.5 1.0 OE2 B:GLU153 3.0 77.0 1.0 CD B:GLU116 3.1 65.0 1.0 ZN B:ZN201 3.1 70.6 1.0 CO B:CO203 3.2 51.2 1.0 OE1 B:GLU102 3.3 48.4 1.0 OE1 B:GLU116 3.7 62.6 1.0 OE2 B:GLU105 4.0 56.0 1.0 CG B:GLU113 4.1 56.9 1.0 CG B:GLU153 4.2 66.8 1.0 CG B:GLU102 4.3 53.3 1.0 CG B:GLU116 4.3 60.4 1.0 OE1 B:GLU105 4.3 54.2 1.0 CD B:GLU105 4.4 67.4 1.0 SD B:MET149 4.4 78.6 1.0 CG B:MET149 4.5 83.9 1.0 CB B:GLU116 4.5 65.1 1.0 CB B:MET149 4.6 80.5 1.0 O C:GLY87 4.6 48.7 1.0 CB B:GLU153 4.7 63.4 1.0 NE2 C:HIS89 4.8 57.4 1.0 CB B:GLU113 4.8 60.5 1.0 CA B:GLU113 4.9 58.9 1.0

Cobalt binding site 4 out of 18 in the Crystal Structure of Cytoplasmic Metal Binding Domain with Cobalt Ions


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 4 of Crystal Structure of Cytoplasmic Metal Binding Domain with Cobalt Ions within 5.0Å range: probe atom residue distance (Å) B Occ B:Co203 b:51.2 occ:1.00 OE2 B:GLU113 1.9 46.0 1.0 N C:GLY87 2.0 66.9 1.0 OE1 B:GLU116 2.1 62.6 1.0 O C:GLY87 2.1 48.7 1.0 O B:HOH301 2.1 40.9 1.0 OE1 B:GLU105 2.5 54.2 1.0 CD B:GLU116 2.8 65.0 1.0 C C:GLY87 2.8 56.9 1.0 OE2 B:GLU116 2.9 52.4 1.0 CA C:GLY87 2.9 59.8 1.0 CD B:GLU113 3.1 58.0 1.0 CO B:CO202 3.2 49.0 1.0 CD B:GLU105 3.3 67.4 1.0 ZN B:ZN201 3.6 70.6 1.0 OE1 B:GLU113 3.7 53.4 1.0 OE2 B:GLU105 3.9 56.0 1.0 N C:SER88 4.1 56.9 1.0 OE2 B:GLU153 4.1 77.0 1.0 CG B:GLU105 4.2 65.8 1.0 CG B:GLU116 4.3 60.4 1.0 CG B:GLU113 4.3 56.9 1.0 CB B:GLU105 4.5 67.6 1.0 OE2 B:GLU102 4.6 41.6 1.0 NE2 C:HIS89 4.6 57.4 1.0 CD2 C:HIS89 4.7 61.4 1.0 CG2 B:THR112 4.7 65.1 1.0 OE1 B:GLU153 4.7 51.2 1.0 CD B:GLU153 4.9 63.0 1.0 OE1 B:GLU102 4.9 48.4 1.0 O C:SER88 4.9 63.3 1.0 CA C:SER88 4.9 63.8 1.0

Cobalt binding site 5 out of 18 in the Crystal Structure of Cytoplasmic Metal Binding Domain with Cobalt Ions


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 5 of Crystal Structure of Cytoplasmic Metal Binding Domain with Cobalt Ions within 5.0Å range: probe atom residue distance (Å) B Occ C:Co203 b:52.9 occ:1.00 O C:HOH301 1.8 30.9 1.0 OE2 C:GLU113 2.0 47.5 1.0 OE2 C:GLU153 2.0 50.5 1.0 OE1 C:GLU102 2.1 57.6 1.0 OE1 C:GLU116 2.5 45.8 1.0 SD C:MET149 2.7 76.6 1.0 CD C:GLU102 2.9 51.7 1.0 ZN C:ZN202 2.9 63.6 1.0 CD C:GLU113 2.9 51.6 1.0 CD C:GLU153 2.9 55.4 1.0 OE2 C:GLU102 3.0 40.7 1.0 OE1 C:GLU153 3.2 48.6 1.0 OE1 C:GLU113 3.2 35.7 1.0 CO C:CO204 3.3 53.5 1.0 CD C:GLU116 3.7 55.2 1.0 CG C:MET149 4.0 75.4 1.0 CE C:MET149 4.1 83.5 1.0 CB C:MET149 4.1 66.5 1.0 NE2 B:HIS89 4.2 48.5 1.0 OE2 C:GLU116 4.3 50.1 1.0 CG C:GLU113 4.3 46.4 1.0 CG C:GLU102 4.3 53.3 1.0 OE2 C:GLU105 4.3 68.3 1.0 CG C:GLU153 4.4 54.8 1.0 O B:GLY87 4.5 50.1 1.0 CB C:GLU153 4.8 56.1 1.0 CD C:GLU105 4.8 73.8 1.0 CE1 B:HIS89 4.8 59.2 1.0 OE1 C:GLU105 4.8 66.6 1.0 CB C:GLU113 4.8 52.2 1.0 CG C:GLU116 4.9 53.2 1.0 CB C:GLU116 5.0 58.7 1.0

Cobalt binding site 6 out of 18 in the Crystal Structure of Cytoplasmic Metal Binding Domain with Cobalt Ions


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 6 of Crystal Structure of Cytoplasmic Metal Binding Domain with Cobalt Ions within 5.0Å range: probe atom residue distance (Å) B Occ C:Co204 b:53.5 occ:1.00 OE1 C:GLU113 1.7 35.7 1.0 O B:GLY87 1.9 50.1 1.0 O C:HOH301 1.9 30.9 1.0 OE2 C:GLU116 2.4 50.1 1.0 N B:GLY87 2.6 73.3 1.0 C B:GLY87 2.6 61.1 1.0 CA B:GLY87 2.6 57.9 1.0 OE1 C:GLU116 2.7 45.8 1.0 CD C:GLU113 2.8 51.6 1.0 CD C:GLU116 2.9 55.2 1.0 OE1 C:GLU105 3.0 66.6 1.0 ZN C:ZN202 3.1 63.6 1.0 CO C:CO203 3.3 52.9 1.0 OE2 C:GLU113 3.3 47.5 1.0 NE2 B:HIS89 3.6 48.5 1.0 CD C:GLU105 3.6 73.8 1.0 OE1 C:GLU153 3.8 48.6 1.0 N B:SER88 3.9 61.8 1.0 CD2 B:HIS89 4.1 60.7 1.0 CG C:GLU113 4.1 46.4 1.0 OE2 C:GLU105 4.2 68.3 1.0 CG C:GLU116 4.4 53.2 1.0 CG C:GLU105 4.5 72.1 1.0 OE2 C:GLU102 4.6 40.7 1.0 OE2 C:GLU153 4.6 50.5 1.0 CD C:GLU153 4.6 55.4 1.0 CB C:GLU105 4.7 65.8 1.0 CG2 C:THR112 4.7 71.6 1.0 CA B:SER88 4.8 66.3 1.0 CE1 B:HIS89 4.9 59.2 1.0 OE1 C:GLU102 4.9 57.6 1.0 CA C:GLU113 4.9 56.9 1.0

Cobalt binding site 7 out of 18 in the Crystal Structure of Cytoplasmic Metal Binding Domain with Cobalt Ions


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 7 of Crystal Structure of Cytoplasmic Metal Binding Domain with Cobalt Ions within 5.0Å range: probe atom residue distance (Å) B Occ C:Co205 b:80.6 occ:1.00 NE2 C:HIS129 2.1 63.1 1.0 CE1 C:HIS129 2.6 59.9 1.0 OE2 C:GLU127 2.7 79.0 1.0 CD2 C:HIS129 3.3 65.5 1.0 CD C:GLU127 3.7 88.1 1.0 ND1 C:HIS129 3.9 67.5 1.0 OE1 C:GLU130 4.1 79.9 1.0 OE1 C:GLU127 4.1 91.9 1.0 CG C:HIS129 4.2 72.6 1.0 CD C:GLU130 4.6 65.3 1.0 CG C:GLU127 4.9 78.7 1.0 CB C:GLU127 5.0 74.7 1.0 CG C:GLU130 5.0 54.9 1.0

Cobalt binding site 8 out of 18 in the Crystal Structure of Cytoplasmic Metal Binding Domain with Cobalt Ions


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 8 of Crystal Structure of Cytoplasmic Metal Binding Domain with Cobalt Ions within 5.0Å range: probe atom residue distance (Å) B Occ D:Co202 b:59.1 occ:1.00 OE2 D:GLU102 1.9 41.8 1.0 OE1 D:GLU113 2.0 72.6 1.0 OE2 D:GLU116 2.1 54.9 1.0 O D:HOH301 2.3 33.6 1.0 OE2 D:GLU153 2.3 72.8 1.0 SD D:MET149 2.8 70.8 1.0 CD D:GLU113 2.9 76.8 1.0 CD D:GLU102 3.0 55.5 1.0 CD D:GLU153 3.0 73.3 1.0 ZN D:ZN203 3.1 74.2 1.0 OE1 D:GLU153 3.1 73.7 1.0 ZN D:ZN201 3.2 69.0 1.0 CD D:GLU116 3.2 62.5 1.0 OE2 D:GLU113 3.3 82.9 1.0 OE1 D:GLU102 3.4 56.4 1.0 CE D:MET149 3.4 70.2 1.0 OE1 D:GLU116 3.6 62.8 1.0 OE1 D:GLU105 3.7 55.3 1.0 CG D:MET149 4.2 63.2 1.0 CG D:GLU113 4.2 73.4 1.0 CG D:GLU102 4.2 64.4 1.0 CG D:GLU153 4.4 75.7 1.0 CB D:MET149 4.4 62.9 1.0 CG D:GLU116 4.5 54.6 1.0 CB D:GLU113 4.7 68.4 1.0 CB D:GLU116 4.7 56.6 1.0 O A:GLY87 4.8 85.1 1.0 CA D:GLU113 4.8 72.9 1.0 CD D:GLU105 4.9 72.7 1.0 CB D:GLU153 5.0 76.4 1.0

Cobalt binding site 9 out of 18 in the Crystal Structure of Cytoplasmic Metal Binding Domain with Cobalt Ions


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 9 of Crystal Structure of Cytoplasmic Metal Binding Domain with Cobalt Ions within 5.0Å range: probe atom residue distance (Å) B Occ D:Co204 b:78.9 occ:1.00 NE2 D:HIS129 2.2 91.2 1.0 CE1 D:HIS129 2.2 89.7 1.0 OE1 D:GLU127 2.5 79.8 1.0 ND1 D:HIS129 3.5 82.8 1.0 CD2 D:HIS129 3.5 90.4 1.0 CD D:GLU127 3.6 92.3 1.0 CG D:HIS129 4.1 86.1 1.0 OE2 D:GLU127 4.2 82.5 1.0 OE1 D:GLU130 4.6 79.5 1.0 CG D:GLU127 4.8 95.4 1.0 CD D:GLU130 4.9 77.5 1.0 CB D:GLU127 4.9 95.9 1.0

Cobalt binding site 10 out of 18 in the Crystal Structure of Cytoplasmic Metal Binding Domain with Cobalt Ions


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 10 of Crystal Structure of Cytoplasmic Metal Binding Domain with Cobalt Ions within 5.0Å range: probe atom residue distance (Å) B Occ E:Co202 b:55.8 occ:1.00 OE2 E:GLU113 1.8 60.0 1.0 OE1 E:GLU102 2.0 57.4 1.0 OE2 E:GLU153 2.0 63.5 1.0 OE1 E:GLU116 2.2 54.9 1.0 O E:HOH302 2.2 47.2 1.0 SD E:MET149 2.8 62.2 1.0 CD E:GLU113 2.9 56.5 1.0 CD E:GLU102 2.9 53.5 1.0 CD E:GLU153 2.9 57.1 1.0 OE1 E:GLU153 3.1 51.9 1.0 OE2 E:GLU102 3.2 46.2 1.0 ZN E:ZN201 3.2 67.2 1.0 ZN E:ZN203 3.3 70.1 1.0 CD E:GLU116 3.3 53.7 1.0 OE1 E:GLU113 3.3 50.5 1.0 OE2 E:GLU116 3.8 52.1 1.0 CE E:MET149 4.0 67.6 1.0 CG E:MET149 4.0 59.6 1.0 CB E:MET149 4.1 56.1 1.0 CG E:GLU113 4.1 50.2 1.0 CG E:GLU102 4.3 57.6 1.0 CG E:GLU153 4.3 58.1 1.0 CB E:GLU113 4.5 48.8 1.0 CG E:GLU116 4.6 50.0 1.0 CA E:GLU113 4.7 53.2 1.0 CB E:GLU116 4.8 52.0 1.0 CB E:GLU153 4.9 51.1 1.0 OE2 E:GLU105 4.9 67.1 1.0 O H:GLY87 4.9 56.2 1.0

T.Kato, K.Kumazaki, M.Wada, R.Taniguchi, T.Nakane, K.Yamashita, K.Hirata, R.Ishitani, K.Ito, T.Nishizawa, O.Nureki. Crystal Structure of Plant Vacuolar Iron Transporter VIT1. Nat Plants V. 5 308 2019.
ISSN: ESSN 2055-0278
PubMed: 30742036
DOI: 10.1038/S41477-019-0367-2