Cobalt in PDB 6m2g: Sirohydrochlorin Nickelochelatase Cfba in Complex with Cobalt- Sirohydrochlorin

All present enzymatic activity of Sirohydrochlorin Nickelochelatase Cfba in Complex with Cobalt- Sirohydrochlorin:
4.99.1.11; 4.99.1.3;

The structure of Sirohydrochlorin Nickelochelatase Cfba in Complex with Cobalt- Sirohydrochlorin, PDB code: 6m2g was solved by T.Fujishiro, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	48.92 / 2.80
Space group	P 41
Cell size a, b, c (Å), α, β, γ (°)	69.18, 69.18, 81.8, 90, 90, 90
R / Rfree (%)	18.4 / 22.4

The binding sites of Cobalt atom in the Sirohydrochlorin Nickelochelatase Cfba in Complex with Cobalt- Sirohydrochlorin (pdb code 6m2g). This binding sites where shown within 5.0 Angstroms radius around Cobalt atom.
In total only one binding site of Cobalt was determined in the Sirohydrochlorin Nickelochelatase Cfba in Complex with Cobalt- Sirohydrochlorin, PDB code: 6m2g:

Cobalt binding site 1 out of 1 in the Sirohydrochlorin Nickelochelatase Cfba in Complex with Cobalt- Sirohydrochlorin


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 1 of Sirohydrochlorin Nickelochelatase Cfba in Complex with Cobalt- Sirohydrochlorin within 5.0Å range: probe atom residue distance (Å) B Occ A:Co201 b:115.3 occ:1.00 CO A:F0X201 0.0 115.3 1.0 N17 A:F0X201 1.8 144.2 1.0 N16 A:F0X201 1.8 142.2 1.0 N27 A:F0X201 1.9 111.7 1.0 N14 A:F0X201 1.9 104.8 1.0 C5 A:F0X201 2.8 148.6 1.0 C3 A:F0X201 2.8 150.2 1.0 C18 A:F0X201 2.9 138.2 1.0 C8 A:F0X201 2.9 131.1 1.0 C26 A:F0X201 2.9 109.7 1.0 C13 A:F0X201 3.0 91.0 1.0 C28 A:F0X201 3.0 100.0 1.0 C10 A:F0X201 3.0 98.4 1.0 C4 A:F0X201 3.1 143.5 1.0 C25 A:F0X201 3.2 119.5 1.0 NE2 A:HIS9 3.2 83.3 1.0 NE2 B:HIS9 3.3 79.2 1.0 C9 A:F0X201 3.3 113.5 1.0 C29 A:F0X201 3.3 97.6 1.0 CE1 B:HIS9 4.0 76.8 1.0 CD2 A:HIS9 4.0 81.5 1.0 C06 A:F0X201 4.1 139.6 1.0 CE1 A:HIS9 4.1 79.2 1.0 C07 A:F0X201 4.1 128.5 1.0 CD2 B:HIS9 4.1 75.5 1.0 C19 A:F0X201 4.2 143.6 1.0 C2 A:F0X201 4.2 148.9 1.0 NE2 A:HIS75 4.2 102.1 1.0 C12 A:F0X201 4.3 82.1 1.0 C31 A:F0X201 4.3 104.8 1.0 C30 A:F0X201 4.3 92.0 1.0 C11 A:F0X201 4.3 93.7 1.0 NE2 B:HIS75 4.3 98.3 1.0 C50 A:F0X201 4.6 119.7 1.0 CD2 A:HIS75 4.9 90.5 1.0 C55 A:F0X201 4.9 139.4 1.0 ND1 B:HIS9 4.9 75.8 1.0

T.Fujishiro, S.Ogawa. The Nickel-Sirohydrochlorin Formation Mechanismof the Ancestral Class II Chelatase Cfba in COENZYMEF430 Biosynthesis Chem Sci 2021.
ISSN: ESSN 2041-6539
DOI: 10.1039/D0SC05439A