Cobalt in PDB 7njg: Xylose Isomerase Grown Inside Hare Serial Crystallography Chip

All present enzymatic activity of Xylose Isomerase Grown Inside Hare Serial Crystallography Chip:
5.3.1.5;

The structure of Xylose Isomerase Grown Inside Hare Serial Crystallography Chip, PDB code: 7njg was solved by B.Norton-Baker, P.Mehrabi, J.Boger, R.Schonherr, D.Von Stetten, H.Schikora, R.W.Martin, R.J.D.Miller, L.Redecke, E.C.Schulz, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	51.57 / 1.90
Space group	I 2 2 2
Cell size a, b, c (Å), α, β, γ (°)	94.45, 99.75, 103.15, 90, 90, 90
R / Rfree (%)	20.2 / 24.4

The binding sites of Cobalt atom in the Xylose Isomerase Grown Inside Hare Serial Crystallography Chip (pdb code 7njg). This binding sites where shown within 5.0 Angstroms radius around Cobalt atom.
In total only one binding site of Cobalt was determined in the Xylose Isomerase Grown Inside Hare Serial Crystallography Chip, PDB code: 7njg:

Cobalt binding site 1 out of 1 in the Xylose Isomerase Grown Inside Hare Serial Crystallography Chip


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 1 of Xylose Isomerase Grown Inside Hare Serial Crystallography Chip within 5.0Å range: probe atom residue distance (Å) B Occ A:Co401 b:20.1 occ:1.00 OE2 A:GLU217 2.1 15.9 1.0 O A:HOH664 2.1 17.0 1.0 OD2 A:ASP255 2.2 18.4 1.0 OD1 A:ASP257 2.2 16.5 1.0 NE2 A:HIS220 2.5 16.2 1.0 OD1 A:ASP255 2.6 19.4 1.0 CG A:ASP255 2.7 15.8 1.0 CD A:GLU217 3.0 15.4 1.0 CG A:ASP257 3.1 18.7 1.0 CD2 A:HIS220 3.2 10.6 1.0 OD2 A:ASP257 3.2 28.0 1.0 OE1 A:GLU217 3.2 15.1 1.0 CE1 A:HIS220 3.5 16.3 1.0 O A:HOH714 3.9 25.3 1.0 ND2 A:ASN247 4.0 16.7 1.0 O A:HOH553 4.1 13.9 1.0 CB A:ASP255 4.2 16.1 1.0 CG A:HIS220 4.3 12.3 1.0 CG A:GLU217 4.4 11.9 1.0 ND1 A:HIS220 4.5 14.8 1.0 CB A:ASP257 4.5 14.0 1.0 O A:HOH612 4.6 33.1 1.0 CE A:LYS183 4.8 14.4 1.0 OD2 A:ASP287 4.9 21.7 1.0 CA A:ASP257 4.9 12.3 1.0 NZ A:LYS183 5.0 18.3 1.0

B.Norton-Baker, P.Mehrabi, J.Boger, R.Schonherr, D.Von Stetten, H.Schikora, A.O.Kwok, R.W.Martin, R.J.D.Miller, L.Redecke, E.C.Schulz. A Simple Vapor-Diffusion Method Enables Protein Crystallization Inside the Hare Serial Crystallography Chip. Acta Crystallogr D Struct V. 77 820 2021BIOL.
ISSN: ISSN 2059-7983
PubMed: 34076595
DOI: 10.1107/S2059798321003855