Cobalt in PDB 8c36: Light-Adapted 2.0 Angstrom Crystal Structure of H132A Variant of Cobalamin Binding Domain Belonging to A Light-Dependent Transcription Regulator Ttcarh Obtained Under Aerobic Conditions

The structure of Light-Adapted 2.0 Angstrom Crystal Structure of H132A Variant of Cobalamin Binding Domain Belonging to A Light-Dependent Transcription Regulator Ttcarh Obtained Under Aerobic Conditions, PDB code: 8c36 was solved by H.Poddar, D.Leys, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	66.85 / 2.00
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	63.405, 70.664, 203.687, 90, 90, 90
R / Rfree (%)	18.1 / 21.8

The binding sites of Cobalt atom in the Light-Adapted 2.0 Angstrom Crystal Structure of H132A Variant of Cobalamin Binding Domain Belonging to A Light-Dependent Transcription Regulator Ttcarh Obtained Under Aerobic Conditions (pdb code 8c36). This binding sites where shown within 5.0 Angstroms radius around Cobalt atom.
In total 4 binding sites of Cobalt where determined in the Light-Adapted 2.0 Angstrom Crystal Structure of H132A Variant of Cobalamin Binding Domain Belonging to A Light-Dependent Transcription Regulator Ttcarh Obtained Under Aerobic Conditions, PDB code: 8c36:
Jump to Cobalt binding site number: 1; 2; 3; 4;

Cobalt binding site 1 out of 4 in the Light-Adapted 2.0 Angstrom Crystal Structure of H132A Variant of Cobalamin Binding Domain Belonging to A Light-Dependent Transcription Regulator Ttcarh Obtained Under Aerobic Conditions


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 1 of Light-Adapted 2.0 Angstrom Crystal Structure of H132A Variant of Cobalamin Binding Domain Belonging to A Light-Dependent Transcription Regulator Ttcarh Obtained Under Aerobic Conditions within 5.0Å range: probe atom residue distance (Å) B Occ A:Co301 b:42.0 occ:1.00 CO A:B12301 0.0 42.0 1.0 N21 A:B12301 1.8 43.9 1.0 N23 A:B12301 1.9 42.9 1.0 N22 A:B12301 1.9 47.5 1.0 N24 A:B12301 1.9 42.5 1.0 NE2 A:HIS177 1.9 46.6 1.0 O A:HOH410 2.5 55.1 1.0 C19 A:B12301 2.8 42.7 1.0 C4 A:B12301 2.8 48.3 1.0 C14 A:B12301 2.8 42.3 1.0 C1 A:B12301 2.9 43.7 1.0 C6 A:B12301 2.9 46.6 1.0 C11 A:B12301 2.9 44.6 1.0 C9 A:B12301 2.9 46.3 1.0 C16 A:B12301 2.9 40.6 1.0 CE1 A:HIS177 2.9 47.7 1.0 CD2 A:HIS177 3.0 43.3 1.0 C5 A:B12301 3.2 47.3 1.0 C15 A:B12301 3.2 40.4 1.0 C10 A:B12301 3.3 45.0 1.0 C20 A:B12301 3.6 41.9 1.0 ND1 A:HIS177 4.1 44.6 1.0 C2 A:B12301 4.1 48.4 1.0 C3 A:B12301 4.1 51.3 1.0 C18 A:B12301 4.1 41.0 1.0 CG A:HIS177 4.1 42.9 1.0 C13 A:B12301 4.1 41.6 1.0 C8 A:B12301 4.2 49.3 1.0 C7 A:B12301 4.2 51.8 1.0 C12 A:B12301 4.2 44.6 1.0 C17 A:B12301 4.2 42.3 1.0 O A:HOH401 4.3 52.6 1.0 OE2 A:GLU141 4.4 41.5 0.5 C26 A:B12301 4.5 45.1 1.0 C35 A:B12301 4.7 49.6 1.0 C53 A:B12301 4.7 40.1 1.0 C41 A:B12301 4.8 50.2 1.0 C37 A:B12301 4.8 52.8 1.0 C54 A:B12301 5.0 41.4 1.0

Cobalt binding site 2 out of 4 in the Light-Adapted 2.0 Angstrom Crystal Structure of H132A Variant of Cobalamin Binding Domain Belonging to A Light-Dependent Transcription Regulator Ttcarh Obtained Under Aerobic Conditions


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 2 of Light-Adapted 2.0 Angstrom Crystal Structure of H132A Variant of Cobalamin Binding Domain Belonging to A Light-Dependent Transcription Regulator Ttcarh Obtained Under Aerobic Conditions within 5.0Å range: probe atom residue distance (Å) B Occ B:Co301 b:45.8 occ:1.00 CO B:B12301 0.0 45.8 1.0 N21 B:B12301 1.8 47.4 1.0 N23 B:B12301 1.9 44.0 1.0 N22 B:B12301 1.9 47.9 1.0 N24 B:B12301 1.9 51.6 1.0 NE2 B:HIS177 2.0 43.1 1.0 O B:HOH421 2.5 47.8 1.0 C19 B:B12301 2.8 50.5 1.0 C4 B:B12301 2.8 46.8 1.0 C14 B:B12301 2.8 41.8 1.0 C1 B:B12301 2.9 46.8 1.0 C11 B:B12301 2.9 45.4 1.0 C16 B:B12301 2.9 50.3 1.0 C9 B:B12301 2.9 45.3 1.0 C6 B:B12301 2.9 46.8 1.0 CE1 B:HIS177 3.0 43.4 1.0 CD2 B:HIS177 3.0 40.8 1.0 C5 B:B12301 3.3 47.7 1.0 C15 B:B12301 3.3 47.8 1.0 C10 B:B12301 3.3 44.8 1.0 C20 B:B12301 3.6 46.7 1.0 C18 B:B12301 4.1 50.8 1.0 C2 B:B12301 4.1 49.6 1.0 C3 B:B12301 4.1 50.4 1.0 ND1 B:HIS177 4.1 39.9 1.0 C13 B:B12301 4.2 41.4 1.0 C7 B:B12301 4.2 50.4 1.0 CG B:HIS177 4.2 38.3 1.0 C17 B:B12301 4.2 51.8 1.0 C8 B:B12301 4.2 46.8 1.0 C12 B:B12301 4.2 43.0 1.0 OE2 B:GLU141 4.3 59.7 0.5 O B:HOH422 4.5 53.1 1.0 C26 B:B12301 4.5 53.1 1.0 C37 B:B12301 4.6 57.4 1.0 C35 B:B12301 4.8 50.4 1.0 C53 B:B12301 4.8 46.7 1.0 C48 B:B12301 4.9 40.3 1.0 C41 B:B12301 4.9 44.7 1.0 C46 B:B12301 4.9 43.5 1.0

Cobalt binding site 3 out of 4 in the Light-Adapted 2.0 Angstrom Crystal Structure of H132A Variant of Cobalamin Binding Domain Belonging to A Light-Dependent Transcription Regulator Ttcarh Obtained Under Aerobic Conditions


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 3 of Light-Adapted 2.0 Angstrom Crystal Structure of H132A Variant of Cobalamin Binding Domain Belonging to A Light-Dependent Transcription Regulator Ttcarh Obtained Under Aerobic Conditions within 5.0Å range: probe atom residue distance (Å) B Occ C:Co301 b:37.9 occ:1.00 CO C:B12301 0.0 37.9 1.0 N21 C:B12301 1.9 39.3 1.0 N23 C:B12301 1.9 39.4 1.0 N24 C:B12301 1.9 39.9 1.0 N22 C:B12301 1.9 40.8 1.0 NE2 C:HIS177 2.0 39.1 1.0 O C:HOH406 2.6 46.9 1.0 C19 C:B12301 2.8 39.0 1.0 C4 C:B12301 2.8 40.0 1.0 C14 C:B12301 2.9 36.1 1.0 C1 C:B12301 2.9 38.5 1.0 C11 C:B12301 2.9 38.5 1.0 C16 C:B12301 2.9 35.4 1.0 C9 C:B12301 2.9 37.9 1.0 C6 C:B12301 2.9 38.5 1.0 CE1 C:HIS177 3.0 39.2 1.0 CD2 C:HIS177 3.1 36.2 1.0 C15 C:B12301 3.3 38.1 1.0 C10 C:B12301 3.3 38.5 1.0 C5 C:B12301 3.3 38.5 1.0 C20 C:B12301 3.6 35.9 1.0 O C:HOH432 4.0 49.4 1.0 C2 C:B12301 4.1 40.1 1.0 C3 C:B12301 4.1 41.6 1.0 C18 C:B12301 4.1 38.0 1.0 ND1 C:HIS177 4.1 38.5 1.0 C17 C:B12301 4.2 37.5 1.0 C13 C:B12301 4.2 37.3 1.0 CG C:HIS177 4.2 36.5 1.0 C8 C:B12301 4.2 39.5 1.0 C12 C:B12301 4.2 38.7 1.0 C7 C:B12301 4.2 43.3 1.0 OE2 C:GLU141 4.4 37.7 0.5 C26 C:B12301 4.6 44.2 1.0 C53 C:B12301 4.8 39.1 1.0 C37 C:B12301 4.8 50.6 1.0 C35 C:B12301 4.8 40.7 1.0 C54 C:B12301 4.9 37.4 1.0 CD C:GLU141 4.9 35.8 0.5 C41 C:B12301 5.0 35.6 1.0

Cobalt binding site 4 out of 4 in the Light-Adapted 2.0 Angstrom Crystal Structure of H132A Variant of Cobalamin Binding Domain Belonging to A Light-Dependent Transcription Regulator Ttcarh Obtained Under Aerobic Conditions


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 4 of Light-Adapted 2.0 Angstrom Crystal Structure of H132A Variant of Cobalamin Binding Domain Belonging to A Light-Dependent Transcription Regulator Ttcarh Obtained Under Aerobic Conditions within 5.0Å range: probe atom residue distance (Å) B Occ D:Co301 b:39.7 occ:1.00 CO D:B12301 0.0 39.7 1.0 N21 D:B12301 1.8 40.0 1.0 N23 D:B12301 1.9 38.6 1.0 N24 D:B12301 1.9 45.8 1.0 N22 D:B12301 1.9 40.1 1.0 NE2 D:HIS177 2.0 39.9 1.0 O D:HOH431 2.5 44.1 1.0 C19 D:B12301 2.8 46.8 1.0 C4 D:B12301 2.8 44.8 1.0 C14 D:B12301 2.8 38.0 1.0 C1 D:B12301 2.9 43.8 1.0 C11 D:B12301 2.9 39.2 1.0 C9 D:B12301 2.9 41.7 1.0 C16 D:B12301 2.9 40.5 1.0 C6 D:B12301 3.0 42.0 1.0 CD2 D:HIS177 3.0 37.1 1.0 CE1 D:HIS177 3.0 39.9 1.0 C15 D:B12301 3.3 38.6 1.0 C10 D:B12301 3.3 40.0 1.0 C5 D:B12301 3.3 43.4 1.0 C20 D:B12301 3.6 41.9 1.0 C2 D:B12301 4.1 46.8 1.0 C3 D:B12301 4.1 48.9 1.0 C18 D:B12301 4.1 48.0 1.0 O D:HOH424 4.1 51.5 1.0 ND1 D:HIS177 4.2 37.4 1.0 C13 D:B12301 4.2 38.8 1.0 CG D:HIS177 4.2 36.9 1.0 C17 D:B12301 4.2 46.4 1.0 C12 D:B12301 4.2 40.5 1.0 C8 D:B12301 4.2 42.3 1.0 C7 D:B12301 4.3 46.0 1.0 OE2 D:GLU141 4.3 63.3 1.0 C26 D:B12301 4.5 46.2 1.0 C35 D:B12301 4.8 47.1 1.0 C53 D:B12301 4.8 36.9 1.0 C37 D:B12301 4.8 49.0 1.0 C41 D:B12301 4.9 39.9 1.0 C48 D:B12301 4.9 38.2 1.0 C46 D:B12301 5.0 42.3 1.0 CD D:GLU141 5.0 65.8 1.0

H.Poddar, D.Leys. Light-Adapted 2.0 Angstrom Crystal Structure of H132A Variant of Cobalamin Binding Domain Belonging to A Light-Dependent Transcription Regulator Ttcarh Obtained Under Aerobic Conditions To Be Published.