Cobalt in PDB 8c37: An Intermediate Light Exposed 2.15 Angstrom Crystal Structure of H132A Variant of Cobalamin Binding Domain Belonging to A Light-Dependent Transcription Regulator Ttcarh Obtained Under Anaerobic Conditions

The structure of An Intermediate Light Exposed 2.15 Angstrom Crystal Structure of H132A Variant of Cobalamin Binding Domain Belonging to A Light-Dependent Transcription Regulator Ttcarh Obtained Under Anaerobic Conditions, PDB code: 8c37 was solved by H.Poddar, D.Leys, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	59.18 / 2.15
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	61.821, 69.683, 201.809, 90, 90, 90
R / Rfree (%)	19.3 / 23

The binding sites of Cobalt atom in the An Intermediate Light Exposed 2.15 Angstrom Crystal Structure of H132A Variant of Cobalamin Binding Domain Belonging to A Light-Dependent Transcription Regulator Ttcarh Obtained Under Anaerobic Conditions (pdb code 8c37). This binding sites where shown within 5.0 Angstroms radius around Cobalt atom.
In total 5 binding sites of Cobalt where determined in the An Intermediate Light Exposed 2.15 Angstrom Crystal Structure of H132A Variant of Cobalamin Binding Domain Belonging to A Light-Dependent Transcription Regulator Ttcarh Obtained Under Anaerobic Conditions, PDB code: 8c37:
Jump to Cobalt binding site number: 1; 2; 3; 4; 5;

Cobalt binding site 1 out of 5 in the An Intermediate Light Exposed 2.15 Angstrom Crystal Structure of H132A Variant of Cobalamin Binding Domain Belonging to A Light-Dependent Transcription Regulator Ttcarh Obtained Under Anaerobic Conditions


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 1 of An Intermediate Light Exposed 2.15 Angstrom Crystal Structure of H132A Variant of Cobalamin Binding Domain Belonging to A Light-Dependent Transcription Regulator Ttcarh Obtained Under Anaerobic Conditions within 5.0Å range: probe atom residue distance (Å) B Occ A:Co301 b:51.2 occ:1.00 CO A:B12301 0.0 51.2 1.0 N23 A:B12301 1.8 46.8 1.0 N21 A:B12301 1.9 54.2 1.0 N24 A:B12301 1.9 54.9 1.0 N22 A:B12301 1.9 46.7 1.0 C19 A:B12301 2.7 55.4 1.0 C14 A:B12301 2.8 47.0 1.0 C1 A:B12301 2.8 58.0 1.0 C9 A:B12301 2.8 53.8 1.0 C11 A:B12301 2.8 47.0 1.0 C16 A:B12301 2.9 54.0 1.0 C4 A:B12301 2.9 54.3 1.0 C6 A:B12301 2.9 57.2 1.0 C10 A:B12301 3.2 50.6 1.0 C15 A:B12301 3.2 46.3 1.0 C5 A:B12301 3.4 60.2 1.0 C20 A:B12301 3.4 64.3 1.0 NE2 A:HIS177 3.7 48.5 1.0 O A:HOH435 4.0 51.2 1.0 C2 A:B12301 4.0 61.1 1.0 C13 A:B12301 4.1 46.3 1.0 CD2 A:HIS177 4.1 45.9 1.0 C17 A:B12301 4.1 57.5 1.0 C7 A:B12301 4.1 60.8 1.0 C18 A:B12301 4.1 56.5 1.0 C8 A:B12301 4.1 58.5 1.0 C12 A:B12301 4.1 51.5 1.0 C3 A:B12301 4.1 59.6 1.0 C48 A:B12301 4.4 50.6 1.0 C26 A:B12301 4.5 58.2 1.0 C37 A:B12301 4.5 56.2 1.0 C53 A:B12301 4.7 47.2 1.0 C46 A:B12301 4.8 52.0 1.0 C55 A:B12301 4.9 59.8 1.0 C35 A:B12301 4.9 61.6 1.0 CE1 A:HIS177 5.0 48.3 1.0 O58 A:B12301 5.0 52.4 1.0

Cobalt binding site 2 out of 5 in the An Intermediate Light Exposed 2.15 Angstrom Crystal Structure of H132A Variant of Cobalamin Binding Domain Belonging to A Light-Dependent Transcription Regulator Ttcarh Obtained Under Anaerobic Conditions


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 2 of An Intermediate Light Exposed 2.15 Angstrom Crystal Structure of H132A Variant of Cobalamin Binding Domain Belonging to A Light-Dependent Transcription Regulator Ttcarh Obtained Under Anaerobic Conditions within 5.0Å range: probe atom residue distance (Å) B Occ B:Co301 b:74.5 occ:1.00 CO B:B12301 0.0 74.5 1.0 N21 B:B12301 1.9 84.8 1.0 N23 B:B12301 1.9 68.8 1.0 N24 B:B12301 1.9 72.1 1.0 N22 B:B12301 1.9 75.4 1.0 C19 B:B12301 2.7 76.8 1.0 C14 B:B12301 2.8 64.6 1.0 C1 B:B12301 2.8 84.3 1.0 O B:HOH416 2.8 71.9 1.0 C4 B:B12301 2.9 79.6 1.0 C11 B:B12301 2.9 59.4 1.0 C16 B:B12301 2.9 70.7 1.0 C9 B:B12301 2.9 70.1 1.0 C6 B:B12301 3.0 81.5 1.0 C15 B:B12301 3.2 66.1 1.0 C5 B:B12301 3.3 83.9 1.0 C10 B:B12301 3.3 56.0 1.0 C20 B:B12301 3.4 82.2 1.0 CE1 B:HIS177 3.8 73.6 1.0 C13 B:B12301 4.1 70.2 1.0 C18 B:B12301 4.1 76.0 1.0 C2 B:B12301 4.1 82.3 1.0 C3 B:B12301 4.2 79.8 1.0 C12 B:B12301 4.2 69.6 1.0 C17 B:B12301 4.2 72.2 1.0 C7 B:B12301 4.2 80.2 1.0 C8 B:B12301 4.2 73.3 1.0 NE2 B:HIS177 4.6 74.4 1.0 C26 B:B12301 4.7 90.0 1.0 C53 B:B12301 4.7 65.2 1.0 C35 B:B12301 4.8 87.2 1.0 ND1 B:HIS177 4.8 71.1 1.0 C37 B:B12301 4.8 94.0 1.0 C47 B:B12301 4.9 70.8 1.0

Cobalt binding site 3 out of 5 in the An Intermediate Light Exposed 2.15 Angstrom Crystal Structure of H132A Variant of Cobalamin Binding Domain Belonging to A Light-Dependent Transcription Regulator Ttcarh Obtained Under Anaerobic Conditions


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 3 of An Intermediate Light Exposed 2.15 Angstrom Crystal Structure of H132A Variant of Cobalamin Binding Domain Belonging to A Light-Dependent Transcription Regulator Ttcarh Obtained Under Anaerobic Conditions within 5.0Å range: probe atom residue distance (Å) B Occ C:Co301 b:62.8 occ:1.00 CO C:B12301 0.0 62.8 1.0 N23 C:B12301 1.9 68.4 1.0 N21 C:B12301 1.9 62.6 1.0 N24 C:B12301 1.9 60.5 1.0 N22 C:B12301 2.0 65.2 1.0 NE2 C:HIS177 2.5 64.0 1.0 C19 C:B12301 2.7 60.4 1.0 C14 C:B12301 2.8 68.8 1.0 C16 C:B12301 2.8 59.6 1.0 C4 C:B12301 2.9 63.1 1.0 C1 C:B12301 2.9 61.1 1.0 C11 C:B12301 2.9 72.1 1.0 C9 C:B12301 3.0 63.7 1.0 C6 C:B12301 3.0 66.0 1.0 C15 C:B12301 3.2 62.7 1.0 C10 C:B12301 3.3 69.3 1.0 C5 C:B12301 3.4 64.9 1.0 CE1 C:HIS177 3.4 61.2 1.0 CD2 C:HIS177 3.5 63.6 1.0 C20 C:B12301 3.5 60.8 1.0 O C:HOH462 3.8 64.2 1.0 C18 C:B12301 4.0 61.8 1.0 C17 C:B12301 4.1 65.5 1.0 C2 C:B12301 4.1 54.9 1.0 C3 C:B12301 4.1 62.1 1.0 C13 C:B12301 4.2 62.3 1.0 C12 C:B12301 4.2 67.4 1.0 C8 C:B12301 4.3 71.8 1.0 C7 C:B12301 4.3 76.3 1.0 C26 C:B12301 4.5 66.2 1.0 ND1 C:HIS177 4.5 58.5 1.0 CG C:HIS177 4.6 59.4 1.0 C53 C:B12301 4.7 56.2 1.0 C35 C:B12301 4.9 61.6 1.0 C55 C:B12301 4.9 68.3 1.0 C54 C:B12301 4.9 64.4 1.0

Cobalt binding site 4 out of 5 in the An Intermediate Light Exposed 2.15 Angstrom Crystal Structure of H132A Variant of Cobalamin Binding Domain Belonging to A Light-Dependent Transcription Regulator Ttcarh Obtained Under Anaerobic Conditions


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 4 of An Intermediate Light Exposed 2.15 Angstrom Crystal Structure of H132A Variant of Cobalamin Binding Domain Belonging to A Light-Dependent Transcription Regulator Ttcarh Obtained Under Anaerobic Conditions within 5.0Å range: probe atom residue distance (Å) B Occ D:Co301 b:57.6 occ:0.50 CO D:B12301 0.0 57.6 0.5 C11 D:B12301 1.3 91.2 0.5 C12 D:B12301 1.8 90.1 0.5 N21 D:B12301 1.8 61.2 0.5 N23 D:B12301 1.9 57.8 0.5 N24 D:B12301 1.9 62.9 0.5 N22 D:B12301 1.9 58.9 0.5 C46 D:B12301 2.0 84.9 0.5 N23 D:B12301 2.0 88.3 0.5 C10 D:B12301 2.0 88.6 0.5 C13 D:B12301 2.2 89.1 0.5 C14 D:B12301 2.4 87.7 0.5 C48 D:B12301 2.6 90.5 0.5 NE2 D:HIS177 2.6 78.2 1.0 C19 D:B12301 2.7 65.6 0.5 C14 D:B12301 2.8 57.6 0.5 C1 D:B12301 2.8 63.5 0.5 C4 D:B12301 2.8 65.5 0.5 C16 D:B12301 2.9 62.0 0.5 C11 D:B12301 2.9 56.1 0.5 C9 D:B12301 2.9 57.8 0.5 C6 D:B12301 2.9 59.2 0.5 O44 D:B12301 3.0 75.7 0.5 C9 D:B12301 3.1 89.4 0.5 C15 D:B12301 3.2 59.8 0.5 C47 D:B12301 3.3 87.6 0.5 C10 D:B12301 3.3 57.9 0.5 C5 D:B12301 3.3 62.4 0.5 CE1 D:HIS177 3.3 75.3 1.0 C20 D:B12301 3.6 62.6 0.5 CO D:B12301 3.6 86.9 0.5 N22 D:B12301 3.6 90.1 0.5 CD2 D:HIS177 3.6 73.9 1.0 C15 D:B12301 3.7 83.1 0.5 C2 D:B12301 4.0 67.6 0.5 C43 D:B12301 4.1 82.9 0.5 C49 D:B12301 4.1 88.5 0.5 C18 D:B12301 4.1 67.6 0.5 C17 D:B12301 4.1 65.5 0.5 C3 D:B12301 4.1 67.6 0.5 C13 D:B12301 4.2 52.7 0.5 C7 D:B12301 4.2 59.7 0.5 C12 D:B12301 4.2 53.4 0.5 C8 D:B12301 4.2 57.0 0.5 C26 D:B12301 4.3 67.6 0.5 C8 D:B12301 4.5 87.8 0.5 ND1 D:HIS177 4.5 71.6 1.0 C53 D:B12301 4.5 82.3 0.5 C16 D:B12301 4.5 84.6 0.5 C37 D:B12301 4.6 60.7 0.5 CG D:HIS177 4.7 69.8 1.0 N24 D:B12301 4.7 87.3 0.5 C53 D:B12301 4.7 59.2 0.5 N45 D:B12301 4.8 81.3 0.5 C35 D:B12301 4.8 63.7 0.5 C41 D:B12301 4.8 87.2 0.5 C46 D:B12301 5.0 52.8 0.5 C50 D:B12301 5.0 88.0 0.5 C6 D:B12301 5.0 87.7 0.5 C41 D:B12301 5.0 54.0 0.5

Cobalt binding site 5 out of 5 in the An Intermediate Light Exposed 2.15 Angstrom Crystal Structure of H132A Variant of Cobalamin Binding Domain Belonging to A Light-Dependent Transcription Regulator Ttcarh Obtained Under Anaerobic Conditions


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 5 of An Intermediate Light Exposed 2.15 Angstrom Crystal Structure of H132A Variant of Cobalamin Binding Domain Belonging to A Light-Dependent Transcription Regulator Ttcarh Obtained Under Anaerobic Conditions within 5.0Å range: probe atom residue distance (Å) B Occ D:Co301 b:86.9 occ:0.50 CO D:B12301 0.0 86.9 0.5 C2 D:B12301 0.8 67.6 0.5 C1 D:B12301 1.0 63.5 0.5 C25 D:B12301 1.8 68.6 0.5 N21 D:B12301 1.8 90.0 0.5 C26 D:B12301 1.8 67.6 0.5 N23 D:B12301 1.8 88.3 0.5 C19 D:B12301 1.9 65.6 0.5 N24 D:B12301 1.9 87.3 0.5 N22 D:B12301 1.9 90.1 0.5 C20 D:B12301 2.1 62.6 0.5 N21 D:B12301 2.1 61.2 0.5 C3 D:B12301 2.1 67.6 0.5 C4 D:B12301 2.6 65.5 0.5 C19 D:B12301 2.7 85.0 0.5 C18 D:B12301 2.8 67.6 0.5 C4 D:B12301 2.8 91.7 0.5 C14 D:B12301 2.8 87.7 0.5 C16 D:B12301 2.8 84.6 0.5 C11 D:B12301 2.9 91.2 0.5 C1 D:B12301 2.9 88.2 0.5 C9 D:B12301 2.9 89.4 0.5 C6 D:B12301 2.9 87.7 0.5 N24 D:B12301 3.0 62.9 0.5 C30 D:B12301 3.0 67.7 0.5 C27 D:B12301 3.0 67.1 0.5 C60 D:B12301 3.2 70.4 0.5 C15 D:B12301 3.2 83.1 0.5 C10 D:B12301 3.3 88.6 0.5 C5 D:B12301 3.3 90.3 0.5 CO D:B12301 3.6 57.6 0.5 N29 D:B12301 3.7 65.7 0.5 O28 D:B12301 3.8 64.3 0.5 C20 D:B12301 3.8 86.2 0.5 C3 D:B12301 3.9 90.6 0.5 C61 D:B12301 4.0 72.4 0.5 C5 D:B12301 4.0 62.4 0.5 C18 D:B12301 4.0 82.9 0.5 C2 D:B12301 4.0 92.1 0.5 C17 D:B12301 4.0 65.5 0.5 C16 D:B12301 4.0 62.0 0.5 C17 D:B12301 4.0 82.0 0.5 C13 D:B12301 4.1 89.1 0.5 C8 D:B12301 4.1 87.8 0.5 C7 D:B12301 4.2 88.3 0.5 C12 D:B12301 4.2 90.1 0.5 O63 D:B12301 4.3 81.4 0.5 N62 D:B12301 4.3 72.2 0.5 C25 D:B12301 4.4 94.1 0.5 C31 D:B12301 4.5 72.7 0.5 C55 D:B12301 4.7 78.6 0.5 C53 D:B12301 4.7 82.3 0.5 C35 D:B12301 4.7 91.4 0.5 C54 D:B12301 4.7 66.6 0.5 N22 D:B12301 4.8 58.9 0.5 C47 D:B12301 4.8 87.6 0.5 O63 D:B12301 4.8 71.2 0.5 C6 D:B12301 4.8 59.2 0.5 C37 D:B12301 4.8 86.0 0.5 C35 D:B12301 4.9 63.7 0.5

H.Poddar, D.Leys. An Intermediate Light Exposed 2.15 Angstrom Crystal Structure of H132A Variant of Cobalamin Binding Domain Belonging to A Light-Dependent Transcription Regulator Ttcarh Obtained Under Anaerobic Conditions To Be Published.