Cobalt in PDB 8c73: Dark State 1.8 Angstrom Crystal Structure of Cobalamin Binding Domain Belonging to A Light-Dependent Transcription Regulator Ttcarh Obtained Under Aerobic Condition Form Ll

The structure of Dark State 1.8 Angstrom Crystal Structure of Cobalamin Binding Domain Belonging to A Light-Dependent Transcription Regulator Ttcarh Obtained Under Aerobic Condition Form Ll, PDB code: 8c73 was solved by R.Rios-Santacruz, J.P.Colletier, G.Schiro, M.Weik, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.91 / 1.70
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	64.519, 66.501, 205.245, 90, 90, 90
R / Rfree (%)	17.1 / 21

The binding sites of Cobalt atom in the Dark State 1.8 Angstrom Crystal Structure of Cobalamin Binding Domain Belonging to A Light-Dependent Transcription Regulator Ttcarh Obtained Under Aerobic Condition Form Ll (pdb code 8c73). This binding sites where shown within 5.0 Angstroms radius around Cobalt atom.
In total 4 binding sites of Cobalt where determined in the Dark State 1.8 Angstrom Crystal Structure of Cobalamin Binding Domain Belonging to A Light-Dependent Transcription Regulator Ttcarh Obtained Under Aerobic Condition Form Ll, PDB code: 8c73:
Jump to Cobalt binding site number: 1; 2; 3; 4;

Cobalt binding site 1 out of 4 in the Dark State 1.8 Angstrom Crystal Structure of Cobalamin Binding Domain Belonging to A Light-Dependent Transcription Regulator Ttcarh Obtained Under Aerobic Condition Form Ll


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 1 of Dark State 1.8 Angstrom Crystal Structure of Cobalamin Binding Domain Belonging to A Light-Dependent Transcription Regulator Ttcarh Obtained Under Aerobic Condition Form Ll within 5.0Å range: probe atom residue distance (Å) B Occ A:Co402 b:21.0 occ:1.00 CO A:B12402 0.0 21.0 1.0 N21 A:B12402 1.9 22.2 1.0 N24 A:B12402 1.9 18.0 1.0 N23 A:B12402 1.9 18.5 1.0 N22 A:B12402 1.9 20.8 1.0 C5' A:5AD401 2.0 23.9 1.0 NE2 A:HIS177 2.3 21.2 1.0 C19 A:B12402 2.8 22.5 1.0 C1 A:B12402 2.9 23.4 1.0 C14 A:B12402 2.9 18.1 1.0 C4 A:B12402 2.9 21.8 1.0 C11 A:B12402 2.9 21.6 1.0 C9 A:B12402 2.9 21.9 1.0 C16 A:B12402 3.0 19.3 1.0 C6 A:B12402 3.0 22.7 1.0 C4' A:5AD401 3.1 26.4 1.0 CD2 A:HIS177 3.2 25.1 1.0 CE1 A:HIS177 3.3 23.2 1.0 C10 A:B12402 3.3 21.1 1.0 C15 A:B12402 3.4 19.3 1.0 C5 A:B12402 3.4 23.2 1.0 C20 A:B12402 3.5 20.4 1.0 O4' A:5AD401 3.6 24.5 1.0 C18 A:B12402 4.1 22.0 1.0 C2 A:B12402 4.1 20.7 1.0 C3 A:B12402 4.2 21.5 1.0 C17 A:B12402 4.2 22.1 1.0 C12 A:B12402 4.2 21.4 1.0 C13 A:B12402 4.2 19.4 1.0 C8 A:B12402 4.3 21.2 1.0 C7 A:B12402 4.3 23.0 1.0 CG A:HIS177 4.4 22.5 1.0 ND1 A:HIS177 4.4 24.1 1.0 C3' A:5AD401 4.4 24.7 1.0 C26 A:B12402 4.5 20.3 1.0 C37 A:B12402 4.7 23.9 1.0 C53 A:B12402 4.9 21.7 1.0 C35 A:B12402 4.9 23.2 1.0 C41 A:B12402 4.9 21.8 1.0 C1' A:5AD401 4.9 23.4 1.0 C48 A:B12402 4.9 20.5 1.0 C54 A:B12402 5.0 22.1 1.0 O3' A:5AD401 5.0 26.7 1.0

Cobalt binding site 2 out of 4 in the Dark State 1.8 Angstrom Crystal Structure of Cobalamin Binding Domain Belonging to A Light-Dependent Transcription Regulator Ttcarh Obtained Under Aerobic Condition Form Ll


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 2 of Dark State 1.8 Angstrom Crystal Structure of Cobalamin Binding Domain Belonging to A Light-Dependent Transcription Regulator Ttcarh Obtained Under Aerobic Condition Form Ll within 5.0Å range: probe atom residue distance (Å) B Occ B:Co301 b:22.1 occ:1.00 CO B:B12301 0.0 22.1 1.0 N22 B:B12301 1.9 22.3 1.0 N21 B:B12301 1.9 20.4 1.0 N24 B:B12301 1.9 22.7 1.0 N23 B:B12301 1.9 19.5 1.0 C5' B:5AD302 2.0 23.1 1.0 NE2 B:HIS177 2.3 21.4 1.0 C19 B:B12301 2.8 24.4 1.0 C11 B:B12301 2.9 20.2 1.0 C9 B:B12301 2.9 19.0 1.0 C1 B:B12301 2.9 23.1 1.0 C4 B:B12301 2.9 20.2 1.0 C14 B:B12301 2.9 21.0 1.0 C6 B:B12301 2.9 20.8 1.0 C16 B:B12301 3.0 19.7 1.0 C4' B:5AD302 3.1 27.3 1.0 CD2 B:HIS177 3.2 23.4 1.0 C10 B:B12301 3.2 21.6 1.0 CE1 B:HIS177 3.3 23.4 1.0 C5 B:B12301 3.3 21.2 1.0 C15 B:B12301 3.4 19.0 1.0 C20 B:B12301 3.5 21.0 1.0 O4' B:5AD302 3.6 26.8 1.0 C18 B:B12301 4.1 22.8 1.0 C2 B:B12301 4.2 23.5 1.0 C3 B:B12301 4.2 21.5 1.0 C7 B:B12301 4.2 22.4 1.0 C8 B:B12301 4.2 21.0 1.0 C12 B:B12301 4.2 22.1 1.0 C17 B:B12301 4.3 23.1 1.0 C13 B:B12301 4.3 20.7 1.0 CG B:HIS177 4.4 21.3 1.0 ND1 B:HIS177 4.4 22.6 1.0 C3' B:5AD302 4.5 28.0 1.0 C26 B:B12301 4.6 21.9 1.0 C37 B:B12301 4.7 23.3 1.0 C41 B:B12301 4.9 22.6 1.0 C35 B:B12301 4.9 22.6 1.0 C54 B:B12301 4.9 22.3 1.0 C53 B:B12301 5.0 20.5 1.0 C1' B:5AD302 5.0 25.9 1.0 C46 B:B12301 5.0 22.2 1.0

Cobalt binding site 3 out of 4 in the Dark State 1.8 Angstrom Crystal Structure of Cobalamin Binding Domain Belonging to A Light-Dependent Transcription Regulator Ttcarh Obtained Under Aerobic Condition Form Ll


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 3 of Dark State 1.8 Angstrom Crystal Structure of Cobalamin Binding Domain Belonging to A Light-Dependent Transcription Regulator Ttcarh Obtained Under Aerobic Condition Form Ll within 5.0Å range: probe atom residue distance (Å) B Occ C:Co301 b:23.7 occ:1.00 CO C:B12301 0.0 23.7 1.0 N21 C:B12301 1.9 23.3 1.0 N24 C:B12301 1.9 22.9 1.0 N22 C:B12301 1.9 22.8 1.0 N23 C:B12301 1.9 22.2 1.0 C5' C:5AD302 2.0 31.2 1.0 NE2 C:HIS177 2.3 25.4 1.0 C19 C:B12301 2.8 25.6 1.0 C4 C:B12301 2.8 26.9 1.0 C14 C:B12301 2.8 20.6 1.0 C1 C:B12301 2.9 24.0 1.0 C9 C:B12301 2.9 22.8 1.0 C11 C:B12301 2.9 21.7 1.0 C6 C:B12301 2.9 25.2 1.0 C16 C:B12301 2.9 23.6 1.0 C4' C:5AD302 3.1 32.9 1.0 CE1 C:HIS177 3.3 28.1 1.0 C5 C:B12301 3.3 25.0 1.0 CD2 C:HIS177 3.3 23.3 1.0 C15 C:B12301 3.3 23.8 1.0 C10 C:B12301 3.3 23.3 1.0 C20 C:B12301 3.5 23.4 1.0 O4' C:5AD302 3.6 28.9 1.0 C18 C:B12301 4.1 22.7 1.0 C2 C:B12301 4.1 23.6 1.0 C3 C:B12301 4.2 22.2 1.0 C17 C:B12301 4.2 22.6 1.0 C8 C:B12301 4.2 24.3 1.0 C13 C:B12301 4.2 22.7 1.0 C7 C:B12301 4.2 26.3 1.0 C12 C:B12301 4.3 23.4 1.0 ND1 C:HIS177 4.4 23.6 1.0 CG C:HIS177 4.4 23.4 1.0 C3' C:5AD302 4.4 31.3 1.0 C26 C:B12301 4.6 25.3 1.0 C37 C:B12301 4.8 26.8 1.0 C35 C:B12301 4.8 29.9 1.0 C53 C:B12301 4.8 22.0 1.0 O3' C:5AD302 4.9 33.0 1.0 C1' C:5AD302 4.9 27.7 1.0 C54 C:B12301 5.0 21.2 1.0 C41 C:B12301 5.0 23.9 1.0 C46 C:B12301 5.0 24.1 1.0

Cobalt binding site 4 out of 4 in the Dark State 1.8 Angstrom Crystal Structure of Cobalamin Binding Domain Belonging to A Light-Dependent Transcription Regulator Ttcarh Obtained Under Aerobic Condition Form Ll


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 4 of Dark State 1.8 Angstrom Crystal Structure of Cobalamin Binding Domain Belonging to A Light-Dependent Transcription Regulator Ttcarh Obtained Under Aerobic Condition Form Ll within 5.0Å range: probe atom residue distance (Å) B Occ D:Co301 b:28.8 occ:1.00 CO D:B12301 0.0 28.8 1.0 N21 D:B12301 1.9 29.1 1.0 N23 D:B12301 1.9 25.7 1.0 N22 D:B12301 2.0 30.3 1.0 N24 D:B12301 2.0 30.1 1.0 C5' D:5AD302 2.1 37.9 1.0 NE2 D:HIS177 2.2 28.3 1.0 C19 D:B12301 2.8 33.0 1.0 C4 D:B12301 2.8 28.7 1.0 C14 D:B12301 2.8 27.7 1.0 C1 D:B12301 2.8 28.4 1.0 C11 D:B12301 2.9 26.4 1.0 C9 D:B12301 2.9 24.2 1.0 C6 D:B12301 2.9 28.7 1.0 C16 D:B12301 3.0 27.5 1.0 CD2 D:HIS177 3.1 26.4 1.0 C4' D:5AD302 3.2 37.4 1.0 C10 D:B12301 3.2 28.7 1.0 C5 D:B12301 3.3 30.3 1.0 CE1 D:HIS177 3.3 28.2 1.0 C15 D:B12301 3.3 29.0 1.0 C20 D:B12301 3.7 27.6 1.0 O4' D:5AD302 3.7 35.1 1.0 C2 D:B12301 4.0 30.4 1.0 C3 D:B12301 4.1 29.3 1.0 C18 D:B12301 4.1 32.8 1.0 C13 D:B12301 4.1 24.4 1.0 C12 D:B12301 4.2 25.4 1.0 C8 D:B12301 4.2 26.1 1.0 C17 D:B12301 4.2 30.0 1.0 C7 D:B12301 4.2 29.1 1.0 CG D:HIS177 4.3 26.3 1.0 ND1 D:HIS177 4.4 26.7 1.0 C26 D:B12301 4.4 30.9 1.0 C3' D:5AD302 4.5 34.8 1.0 C35 D:B12301 4.7 31.3 1.0 C37 D:B12301 4.7 31.5 1.0 C53 D:B12301 4.8 29.3 1.0 C41 D:B12301 4.9 24.3 1.0 C54 D:B12301 4.9 32.9 1.0 O3' D:5AD302 5.0 39.5 1.0 C46 D:B12301 5.0 26.1 1.0

H.Poddar, R.Rios-Santacruz, D.J.Heyes, M.Shanmugam, A.Brookfield, L.O.Johannissen, C.Levy, S.Zhang, M.Sakuma, J.P.Colletier, S.Hay, G.Schiro, M.Weik, N.Scrutton, D.Leys. Redox Driven B12-Ligand Switch Drives Carh Photoresponse To Be Published.