Cobalt in PDB 8c76: Light-State 2.5 Angstrom Wild-Type X-Ray Crystal Structure of the Cobalamin Binding Domain Belonging to A Light-Dependent Transcription Regulator Ttcarh Obtained Under Aerobic Conditions From A Form 2 Crystal Illuminated During 5 S

The structure of Light-State 2.5 Angstrom Wild-Type X-Ray Crystal Structure of the Cobalamin Binding Domain Belonging to A Light-Dependent Transcription Regulator Ttcarh Obtained Under Aerobic Conditions From A Form 2 Crystal Illuminated During 5 S, PDB code: 8c76 was solved by R.Rios-Santacruz, J.P.Colletier, G.Schiro, M.Weik, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.89 / 2.50
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	61.856, 73.157, 204.86, 90, 90, 90
R / Rfree (%)	18.6 / 23.5

The binding sites of Cobalt atom in the Light-State 2.5 Angstrom Wild-Type X-Ray Crystal Structure of the Cobalamin Binding Domain Belonging to A Light-Dependent Transcription Regulator Ttcarh Obtained Under Aerobic Conditions From A Form 2 Crystal Illuminated During 5 S (pdb code 8c76). This binding sites where shown within 5.0 Angstroms radius around Cobalt atom.
In total 4 binding sites of Cobalt where determined in the Light-State 2.5 Angstrom Wild-Type X-Ray Crystal Structure of the Cobalamin Binding Domain Belonging to A Light-Dependent Transcription Regulator Ttcarh Obtained Under Aerobic Conditions From A Form 2 Crystal Illuminated During 5 S, PDB code: 8c76:
Jump to Cobalt binding site number: 1; 2; 3; 4;

Cobalt binding site 1 out of 4 in the Light-State 2.5 Angstrom Wild-Type X-Ray Crystal Structure of the Cobalamin Binding Domain Belonging to A Light-Dependent Transcription Regulator Ttcarh Obtained Under Aerobic Conditions From A Form 2 Crystal Illuminated During 5 S


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 1 of Light-State 2.5 Angstrom Wild-Type X-Ray Crystal Structure of the Cobalamin Binding Domain Belonging to A Light-Dependent Transcription Regulator Ttcarh Obtained Under Aerobic Conditions From A Form 2 Crystal Illuminated During 5 S within 5.0Å range: probe atom residue distance (Å) B Occ A:Co301 b:61.9 occ:1.00 CO A:B12301 0.0 61.9 1.0 N21 A:B12301 1.8 65.1 1.0 N23 A:B12301 1.8 63.9 1.0 N24 A:B12301 1.9 53.4 1.0 N22 A:B12301 1.9 70.5 1.0 C19 A:B12301 2.7 56.9 1.0 C1 A:B12301 2.7 66.3 1.0 C4 A:B12301 2.8 71.2 1.0 C14 A:B12301 2.8 60.3 1.0 C11 A:B12301 2.9 59.1 1.0 C16 A:B12301 2.9 55.2 1.0 C6 A:B12301 2.9 73.7 1.0 C9 A:B12301 2.9 71.0 1.0 NE2 A:HIS132 3.2 76.8 1.0 C5 A:B12301 3.2 70.0 1.0 C10 A:B12301 3.2 62.8 1.0 C15 A:B12301 3.3 64.0 1.0 C20 A:B12301 3.4 57.9 1.0 O A:HOH411 3.7 60.6 1.0 C2 A:B12301 4.0 78.2 1.0 C18 A:B12301 4.0 59.7 1.0 CE1 A:HIS132 4.1 87.0 1.0 C3 A:B12301 4.1 78.0 1.0 C17 A:B12301 4.1 57.9 1.0 C13 A:B12301 4.1 57.8 1.0 C7 A:B12301 4.2 78.5 1.0 C12 A:B12301 4.2 64.8 1.0 CD2 A:HIS132 4.2 91.6 1.0 C8 A:B12301 4.2 72.6 1.0 C26 A:B12301 4.4 75.6 1.0 C37 A:B12301 4.6 92.8 1.0 C46 A:B12301 4.7 59.2 1.0 C35 A:B12301 4.7 70.0 1.0 C53 A:B12301 4.8 50.6 1.0 C48 A:B12301 4.8 58.3 1.0 C55 A:B12301 4.9 67.2 1.0

Cobalt binding site 2 out of 4 in the Light-State 2.5 Angstrom Wild-Type X-Ray Crystal Structure of the Cobalamin Binding Domain Belonging to A Light-Dependent Transcription Regulator Ttcarh Obtained Under Aerobic Conditions From A Form 2 Crystal Illuminated During 5 S


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 2 of Light-State 2.5 Angstrom Wild-Type X-Ray Crystal Structure of the Cobalamin Binding Domain Belonging to A Light-Dependent Transcription Regulator Ttcarh Obtained Under Aerobic Conditions From A Form 2 Crystal Illuminated During 5 S within 5.0Å range: probe atom residue distance (Å) B Occ B:Co301 b:73.9 occ:1.00 CO B:B12301 0.0 73.9 1.0 N23 B:B12301 1.8 75.8 1.0 N21 B:B12301 1.8 89.4 1.0 N24 B:B12301 1.9 81.6 1.0 N22 B:B12301 1.9 93.9 1.0 C19 B:B12301 2.7 77.8 1.0 C14 B:B12301 2.8 73.2 1.0 C11 B:B12301 2.8 82.9 1.0 C1 B:B12301 2.8 78.4 1.0 C4 B:B12301 2.8 86.5 1.0 C9 B:B12301 2.8 92.0 1.0 C6 B:B12301 2.9 96.3 1.0 C16 B:B12301 2.9 72.7 1.0 C10 B:B12301 3.2 85.6 1.0 C15 B:B12301 3.2 74.0 1.0 C5 B:B12301 3.3 95.0 1.0 NE2 B:HIS132 3.3 67.5 1.0 C20 B:B12301 3.6 54.7 1.0 C2 B:B12301 4.0 85.4 1.0 C18 B:B12301 4.1 80.9 1.0 C13 B:B12301 4.1 74.3 1.0 C3 B:B12301 4.1 86.3 1.0 CE1 B:HIS132 4.1 88.6 1.0 C12 B:B12301 4.1 82.1 1.0 C7 B:B12301 4.1 93.3 1.0 C17 B:B12301 4.2 70.8 1.0 C8 B:B12301 4.2 89.4 1.0 CD2 B:HIS132 4.3 73.4 1.0 C26 B:B12301 4.4 86.9 1.0 CE1 B:HIS177 4.5 88.3 1.0 C37 B:B12301 4.6 92.0 1.0 C48 B:B12301 4.7 65.2 1.0 C35 B:B12301 4.7 80.0 1.0 C53 B:B12301 4.8 55.3 1.0 C42 B:B12301 4.8 97.3 1.0 C46 B:B12301 4.8 71.5 1.0

Cobalt binding site 3 out of 4 in the Light-State 2.5 Angstrom Wild-Type X-Ray Crystal Structure of the Cobalamin Binding Domain Belonging to A Light-Dependent Transcription Regulator Ttcarh Obtained Under Aerobic Conditions From A Form 2 Crystal Illuminated During 5 S


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 3 of Light-State 2.5 Angstrom Wild-Type X-Ray Crystal Structure of the Cobalamin Binding Domain Belonging to A Light-Dependent Transcription Regulator Ttcarh Obtained Under Aerobic Conditions From A Form 2 Crystal Illuminated During 5 S within 5.0Å range: probe atom residue distance (Å) B Occ C:Co301 b:106.8 occ:1.00 CO C:B12301 0.0 106.8 1.0 N21 C:B12301 1.8 109.0 1.0 N23 C:B12301 1.8 112.8 1.0 N24 C:B12301 1.9 99.0 1.0 N22 C:B12301 1.9 126.6 1.0 C19 C:B12301 2.8 98.0 1.0 C4 C:B12301 2.8 116.2 1.0 C1 C:B12301 2.8 100.2 1.0 C14 C:B12301 2.8 103.2 1.0 C11 C:B12301 2.8 104.1 1.0 C9 C:B12301 2.8 120.3 1.0 C6 C:B12301 2.8 136.0 1.0 C16 C:B12301 2.9 95.9 1.0 C10 C:B12301 3.1 123.8 1.0 C5 C:B12301 3.2 131.3 1.0 C15 C:B12301 3.3 101.6 1.0 C20 C:B12301 3.4 88.0 1.0 NE2 C:HIS132 3.4 100.0 1.0 C2 C:B12301 4.0 96.0 1.0 C3 C:B12301 4.1 100.3 1.0 C13 C:B12301 4.1 92.3 1.0 C18 C:B12301 4.1 88.6 1.0 C7 C:B12301 4.1 129.5 1.0 C12 C:B12301 4.1 97.8 1.0 C8 C:B12301 4.2 119.9 1.0 C17 C:B12301 4.3 93.4 1.0 CE1 C:HIS132 4.3 113.5 1.0 CD2 C:HIS132 4.5 98.9 1.0 C26 C:B12301 4.6 101.0 1.0 C35 C:B12301 4.6 91.1 1.0 C37 C:B12301 4.6 125.7 1.0 C53 C:B12301 4.8 85.2 1.0 C30 C:B12301 4.9 113.5 1.0

Cobalt binding site 4 out of 4 in the Light-State 2.5 Angstrom Wild-Type X-Ray Crystal Structure of the Cobalamin Binding Domain Belonging to A Light-Dependent Transcription Regulator Ttcarh Obtained Under Aerobic Conditions From A Form 2 Crystal Illuminated During 5 S


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 4 of Light-State 2.5 Angstrom Wild-Type X-Ray Crystal Structure of the Cobalamin Binding Domain Belonging to A Light-Dependent Transcription Regulator Ttcarh Obtained Under Aerobic Conditions From A Form 2 Crystal Illuminated During 5 S within 5.0Å range: probe atom residue distance (Å) B Occ D:Co301 b:100.5 occ:1.00 CO D:B12301 0.0 100.5 1.0 N21 D:B12301 1.8 134.2 1.0 N23 D:B12301 1.9 109.3 1.0 N24 D:B12301 1.9 104.2 1.0 N22 D:B12301 1.9 105.5 1.0 C19 D:B12301 2.7 123.3 1.0 C9 D:B12301 2.8 92.1 1.0 C1 D:B12301 2.8 147.2 1.0 C4 D:B12301 2.8 132.4 1.0 C6 D:B12301 2.8 112.7 1.0 C14 D:B12301 2.8 94.2 1.0 C11 D:B12301 2.9 95.1 1.0 C16 D:B12301 2.9 100.4 1.0 C5 D:B12301 3.2 102.6 1.0 C10 D:B12301 3.3 80.2 1.0 C15 D:B12301 3.3 87.0 1.0 C20 D:B12301 3.6 146.3 1.0 C7 D:B12301 3.9 117.0 1.0 C8 D:B12301 3.9 110.8 1.0 C2 D:B12301 3.9 169.8 1.0 C3 D:B12301 4.0 164.4 1.0 C18 D:B12301 4.0 109.1 1.0 C17 D:B12301 4.1 104.5 1.0 C12 D:B12301 4.1 93.5 1.0 C13 D:B12301 4.1 96.9 1.0 C37 D:B12301 4.2 147.6 1.0 C26 D:B12301 4.5 174.7 1.0 C46 D:B12301 4.6 95.0 1.0 C35 D:B12301 4.6 79.8 1.0 C48 D:B12301 4.8 98.8 1.0 C53 D:B12301 4.8 68.8 1.0 C56 D:B12301 4.8 101.8 1.0

H.Poddar, R.Rios-Santacruz, D.J.Heyes, M.Shanmugam, A.Brookfield, L.O.Johannissen, C.Levy, S.Zhang, M.Sakuma, J.P.Colletier, S.Hay, G.Schiro, M.Weik, N.Scrutton, D.Leys. Redox Driven B12-Ligand Switch Drives Carh Photoresponse To Be Published.