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Cobalt in PDB 8yes: Crystal Structure of Adenosylcobinamide Kinase / Adenosylcobinamide Phosphate Guanylyltransferase Complexed with Adenosylcobinamide- Phosphate

Enzymatic activity of Crystal Structure of Adenosylcobinamide Kinase / Adenosylcobinamide Phosphate Guanylyltransferase Complexed with Adenosylcobinamide- Phosphate

All present enzymatic activity of Crystal Structure of Adenosylcobinamide Kinase / Adenosylcobinamide Phosphate Guanylyltransferase Complexed with Adenosylcobinamide- Phosphate:
2.7.1.156; 2.7.7.62;

Protein crystallography data

The structure of Crystal Structure of Adenosylcobinamide Kinase / Adenosylcobinamide Phosphate Guanylyltransferase Complexed with Adenosylcobinamide- Phosphate, PDB code: 8yes was solved by Y.Nam, H.Do, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 47.96 / 2.60
Space group P 2 21 21
Cell size a, b, c (Å), α, β, γ (°) 99.213, 102.922, 129.228, 90, 90, 90
R / Rfree (%) 19.3 / 26.3

Cobalt Binding Sites:

The binding sites of Cobalt atom in the Crystal Structure of Adenosylcobinamide Kinase / Adenosylcobinamide Phosphate Guanylyltransferase Complexed with Adenosylcobinamide- Phosphate (pdb code 8yes). This binding sites where shown within 5.0 Angstroms radius around Cobalt atom.
In total 6 binding sites of Cobalt where determined in the Crystal Structure of Adenosylcobinamide Kinase / Adenosylcobinamide Phosphate Guanylyltransferase Complexed with Adenosylcobinamide- Phosphate, PDB code: 8yes:
Jump to Cobalt binding site number: 1; 2; 3; 4; 5; 6;

Cobalt binding site 1 out of 6 in 8yes

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Cobalt binding site 1 out of 6 in the Crystal Structure of Adenosylcobinamide Kinase / Adenosylcobinamide Phosphate Guanylyltransferase Complexed with Adenosylcobinamide- Phosphate


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 1 of Crystal Structure of Adenosylcobinamide Kinase / Adenosylcobinamide Phosphate Guanylyltransferase Complexed with Adenosylcobinamide- Phosphate within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Co201

b:45.0
occ:1.00
CO A:B12201 0.0 45.0 1.0
N23 A:B12201 1.8 48.5 1.0
N22 A:B12201 1.8 42.2 1.0
N21 A:B12201 1.9 48.0 1.0
N24 A:B12201 2.0 45.0 1.0
C5' A:5AD202 2.1 60.8 1.0
C9 A:B12201 2.8 47.6 1.0
C14 A:B12201 2.8 47.0 1.0
C4 A:B12201 2.8 45.3 1.0
C19 A:B12201 2.8 44.6 1.0
C11 A:B12201 2.8 46.2 1.0
C6 A:B12201 2.8 46.8 1.0
C1 A:B12201 2.9 49.3 1.0
C16 A:B12201 3.0 50.0 1.0
C5 A:B12201 3.1 48.9 1.0
C10 A:B12201 3.2 44.1 1.0
C15 A:B12201 3.3 50.1 1.0
C4' A:5AD202 3.3 60.3 1.0
C20 A:B12201 3.6 46.7 1.0
O4' A:5AD202 3.8 60.4 1.0
C2 A:B12201 4.0 48.9 1.0
C18 A:B12201 4.1 62.0 1.0
C7 A:B12201 4.1 47.9 1.0
C3 A:B12201 4.1 45.1 1.0
C8 A:B12201 4.1 47.8 1.0
C12 A:B12201 4.1 44.2 1.0
C13 A:B12201 4.1 42.5 1.0
C17 A:B12201 4.2 58.8 1.0
CG1 C:VAL136 4.3 48.8 1.0
C26 A:B12201 4.4 60.1 1.0
C35 A:B12201 4.5 57.7 1.0
C37 A:B12201 4.5 56.4 1.0
C3' A:5AD202 4.7 61.4 1.0
C53 A:B12201 4.7 48.2 1.0
C46 A:B12201 4.8 40.1 1.0
C54 A:B12201 4.8 61.9 1.0
C41 A:B12201 4.8 51.4 1.0
C48 A:B12201 4.9 43.1 1.0

Cobalt binding site 2 out of 6 in 8yes

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Cobalt binding site 2 out of 6 in the Crystal Structure of Adenosylcobinamide Kinase / Adenosylcobinamide Phosphate Guanylyltransferase Complexed with Adenosylcobinamide- Phosphate


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 2 of Crystal Structure of Adenosylcobinamide Kinase / Adenosylcobinamide Phosphate Guanylyltransferase Complexed with Adenosylcobinamide- Phosphate within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Co201

b:50.5
occ:1.00
CO B:B12201 0.0 50.5 1.0
N21 B:B12201 1.8 51.7 1.0
N23 B:B12201 1.9 47.9 1.0
N22 B:B12201 1.9 55.7 1.0
N24 B:B12201 1.9 45.6 1.0
C5' B:5AD202 2.0 59.6 1.0
C19 B:B12201 2.7 45.3 1.0
C4 B:B12201 2.8 53.4 1.0
C1 B:B12201 2.8 47.7 1.0
C14 B:B12201 2.8 46.2 1.0
C11 B:B12201 2.9 47.5 1.0
C16 B:B12201 2.9 46.6 1.0
C9 B:B12201 2.9 51.9 1.0
C6 B:B12201 2.9 54.0 1.0
C4' B:5AD202 3.2 58.8 1.0
C5 B:B12201 3.2 51.8 1.0
C15 B:B12201 3.3 54.8 1.0
C10 B:B12201 3.3 47.9 1.0
C20 B:B12201 3.5 43.0 1.0
O4' B:5AD202 3.6 58.3 1.0
C18 B:B12201 3.9 58.6 1.0
C2 B:B12201 4.0 47.5 1.0
C3 B:B12201 4.0 52.4 1.0
C17 B:B12201 4.1 52.8 1.0
C13 B:B12201 4.2 45.5 1.0
C12 B:B12201 4.2 49.5 1.0
C7 B:B12201 4.2 54.3 1.0
C8 B:B12201 4.2 49.5 1.0
CG1 A:VAL136 4.3 51.8 1.0
C26 B:B12201 4.4 49.4 1.0
C3' B:5AD202 4.5 54.5 1.0
C35 B:B12201 4.7 48.4 1.0
C46 B:B12201 4.7 48.4 1.0
C53 B:B12201 4.8 50.6 1.0
C37 B:B12201 4.8 58.6 1.0
C54 B:B12201 4.8 58.5 1.0
C42 B:B12201 4.8 52.9 1.0
C48 B:B12201 5.0 44.2 1.0
C30 B:B12201 5.0 55.5 1.0

Cobalt binding site 3 out of 6 in 8yes

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Cobalt binding site 3 out of 6 in the Crystal Structure of Adenosylcobinamide Kinase / Adenosylcobinamide Phosphate Guanylyltransferase Complexed with Adenosylcobinamide- Phosphate


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 3 of Crystal Structure of Adenosylcobinamide Kinase / Adenosylcobinamide Phosphate Guanylyltransferase Complexed with Adenosylcobinamide- Phosphate within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Co203

b:45.2
occ:1.00
CO B:B12203 0.0 45.2 1.0
N21 B:B12203 1.8 47.2 1.0
N23 B:B12203 1.9 46.5 1.0
N22 B:B12203 1.9 45.8 1.0
N24 B:B12203 1.9 47.4 1.0
C5' C:5AD201 2.0 49.5 1.0
C19 B:B12203 2.7 45.5 1.0
C4 B:B12203 2.8 47.0 1.0
C14 B:B12203 2.8 45.7 1.0
C1 B:B12203 2.8 50.1 1.0
C6 B:B12203 2.9 50.0 1.0
C11 B:B12203 2.9 48.3 1.0
C9 B:B12203 2.9 53.4 1.0
C16 B:B12203 2.9 50.9 1.0
C4' C:5AD201 3.1 57.8 1.0
C5 B:B12203 3.2 52.1 1.0
C15 B:B12203 3.2 47.8 1.0
C10 B:B12203 3.3 49.0 1.0
C20 B:B12203 3.6 42.4 1.0
O4' C:5AD201 3.7 58.4 1.0
C18 B:B12203 3.9 56.9 1.0
C2 B:B12203 4.0 47.7 1.0
C3 B:B12203 4.1 49.1 1.0
C7 B:B12203 4.2 55.0 1.0
C13 B:B12203 4.2 44.3 1.0
C12 B:B12203 4.2 44.2 1.0
C8 B:B12203 4.2 54.2 1.0
C17 B:B12203 4.2 59.0 1.0
CG2 B:VAL136 4.2 55.6 1.0
C3' C:5AD201 4.5 57.1 1.0
C26 B:B12203 4.5 49.7 1.0
C37 B:B12203 4.6 65.0 1.0
C35 B:B12203 4.7 50.2 1.0
C53 B:B12203 4.7 54.1 1.0
C46 B:B12203 4.8 43.7 1.0
C54 B:B12203 4.9 57.2 1.0
C41 B:B12203 5.0 53.3 1.0
O3' C:5AD201 5.0 58.2 1.0

Cobalt binding site 4 out of 6 in 8yes

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Cobalt binding site 4 out of 6 in the Crystal Structure of Adenosylcobinamide Kinase / Adenosylcobinamide Phosphate Guanylyltransferase Complexed with Adenosylcobinamide- Phosphate


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 4 of Crystal Structure of Adenosylcobinamide Kinase / Adenosylcobinamide Phosphate Guanylyltransferase Complexed with Adenosylcobinamide- Phosphate within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Co201

b:50.9
occ:1.00
CO D:B12201 0.0 50.9 1.0
N21 D:B12201 1.8 54.4 1.0
N23 D:B12201 1.9 54.2 1.0
N22 D:B12201 1.9 47.7 1.0
N24 D:B12201 1.9 46.5 1.0
C5' D:5AD202 2.0 63.0 1.0
C19 D:B12201 2.7 48.7 1.0
C4 D:B12201 2.8 53.0 1.0
C1 D:B12201 2.8 53.0 1.0
C6 D:B12201 2.8 58.5 1.0
C14 D:B12201 2.8 48.5 1.0
C9 D:B12201 2.9 53.3 1.0
C11 D:B12201 2.9 53.3 1.0
C16 D:B12201 2.9 56.5 1.0
C4' D:5AD202 3.2 64.7 1.0
C5 D:B12201 3.2 58.1 1.0
C15 D:B12201 3.3 53.9 1.0
C10 D:B12201 3.3 48.2 1.0
C20 D:B12201 3.4 51.4 1.0
O4' D:5AD202 3.6 62.3 1.0
C18 D:B12201 3.9 60.2 1.0
C2 D:B12201 4.0 49.7 1.0
C3 D:B12201 4.1 50.8 1.0
C7 D:B12201 4.1 63.7 1.0
C12 D:B12201 4.1 49.6 1.0
C8 D:B12201 4.2 59.8 1.0
C13 D:B12201 4.2 51.4 1.0
C17 D:B12201 4.2 57.5 1.0
C37 D:B12201 4.4 71.0 1.0
C26 D:B12201 4.5 48.5 1.0
C3' D:5AD202 4.5 60.6 1.0
C46 D:B12201 4.6 52.9 1.0
C35 D:B12201 4.6 57.4 1.0
C53 D:B12201 4.8 52.1 1.0
C41 D:B12201 4.9 58.0 1.0
C54 D:B12201 4.9 65.0 1.0

Cobalt binding site 5 out of 6 in 8yes

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Cobalt binding site 5 out of 6 in the Crystal Structure of Adenosylcobinamide Kinase / Adenosylcobinamide Phosphate Guanylyltransferase Complexed with Adenosylcobinamide- Phosphate


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 5 of Crystal Structure of Adenosylcobinamide Kinase / Adenosylcobinamide Phosphate Guanylyltransferase Complexed with Adenosylcobinamide- Phosphate within 5.0Å range:
probe atom residue distance (Å) B Occ
E:Co201

b:58.4
occ:1.00
CO E:B12201 0.0 58.4 1.0
N21 E:B12201 1.8 63.9 1.0
N23 E:B12201 1.9 53.2 1.0
N22 E:B12201 1.9 57.4 1.0
N24 E:B12201 1.9 54.6 1.0
C5' E:5AD202 2.0 78.3 1.0
C19 E:B12201 2.7 57.0 1.0
C4 E:B12201 2.8 59.5 1.0
C1 E:B12201 2.8 62.2 1.0
C9 E:B12201 2.8 58.7 1.0
C14 E:B12201 2.8 51.0 1.0
C11 E:B12201 2.8 54.7 1.0
C6 E:B12201 2.9 62.3 1.0
C16 E:B12201 2.9 59.4 1.0
C10 E:B12201 3.2 56.0 1.0
C5 E:B12201 3.2 66.2 1.0
C15 E:B12201 3.2 49.2 1.0
C4' E:5AD202 3.3 81.1 1.0
C20 E:B12201 3.4 61.5 1.0
O4' E:5AD202 3.7 81.1 1.0
C18 E:B12201 3.9 70.8 1.0
C2 E:B12201 4.0 58.5 1.0
C3 E:B12201 4.1 60.0 1.0
C8 E:B12201 4.1 63.7 1.0
C7 E:B12201 4.2 63.0 1.0
C12 E:B12201 4.2 51.3 1.0
C13 E:B12201 4.2 50.9 1.0
C17 E:B12201 4.2 65.7 1.0
C26 E:B12201 4.4 66.8 1.0
C3' E:5AD202 4.6 84.7 1.0
C35 E:B12201 4.6 72.0 1.0
C37 E:B12201 4.7 73.8 1.0
C53 E:B12201 4.7 49.1 1.0
C46 E:B12201 4.8 52.1 1.0
C41 E:B12201 4.9 64.5 1.0
C54 E:B12201 4.9 61.7 1.0

Cobalt binding site 6 out of 6 in 8yes

Go back to Cobalt Binding Sites List in 8yes
Cobalt binding site 6 out of 6 in the Crystal Structure of Adenosylcobinamide Kinase / Adenosylcobinamide Phosphate Guanylyltransferase Complexed with Adenosylcobinamide- Phosphate


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 6 of Crystal Structure of Adenosylcobinamide Kinase / Adenosylcobinamide Phosphate Guanylyltransferase Complexed with Adenosylcobinamide- Phosphate within 5.0Å range:
probe atom residue distance (Å) B Occ
F:Co201

b:60.3
occ:1.00
CO F:B12201 0.0 60.3 1.0
N21 F:B12201 1.8 54.2 1.0
N23 F:B12201 1.9 52.8 1.0
N22 F:B12201 1.9 61.8 1.0
N24 F:B12201 1.9 57.3 1.0
C5' F:5AD202 2.0 73.2 1.0
C19 F:B12201 2.7 57.8 1.0
C4 F:B12201 2.8 60.1 1.0
C14 F:B12201 2.8 53.4 1.0
C1 F:B12201 2.8 53.9 1.0
C11 F:B12201 2.9 56.1 1.0
C9 F:B12201 2.9 62.2 1.0
C16 F:B12201 2.9 55.2 1.0
C6 F:B12201 2.9 64.3 1.0
C15 F:B12201 3.2 54.9 1.0
C10 F:B12201 3.2 64.1 1.0
C5 F:B12201 3.3 60.8 1.0
C4' F:5AD202 3.3 74.5 1.0
C20 F:B12201 3.5 59.2 1.0
O4' F:5AD202 3.8 70.3 1.0
C18 F:B12201 3.9 71.3 1.0
C2 F:B12201 4.0 57.1 1.0
C3 F:B12201 4.1 58.2 1.0
C13 F:B12201 4.2 52.1 1.0
C8 F:B12201 4.2 64.3 1.0
C12 F:B12201 4.2 52.3 1.0
C7 F:B12201 4.2 67.6 1.0
C17 F:B12201 4.2 67.5 1.0
C26 F:B12201 4.6 64.3 1.0
C3' F:5AD202 4.6 74.7 1.0
C37 F:B12201 4.7 77.5 1.0
C53 F:B12201 4.7 57.1 1.0
C35 F:B12201 4.7 61.0 1.0
C41 F:B12201 4.8 61.5 1.0
C54 F:B12201 4.9 72.6 1.0
C48 F:B12201 4.9 57.1 1.0
C46 F:B12201 5.0 52.9 1.0

Reference:

Y.Nam, Y.Y.Ahn, B.M.Kim, K.Kim, J.H.Lee, H.Do. A Structure-Based Mechanism of Adenosylcobinamide Kinase/Adenosylcobinamide Phosphate Guanylyltransferase (Mpacobu) From Methylocapsa Palsarum. Int.J.Biol.Macromol. V. 280 36021 2024.
ISSN: ISSN 0141-8130
PubMed: 39326622
DOI: 10.1016/J.IJBIOMAC.2024.136021
Page generated: Sat Feb 8 17:38:14 2025

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