Cobalt in PDB 1cia: Replacement of Catalytic Histidine-195 of Chloramphenicol Acetyltransferase: Evidence For A General Base Role For Glutamate

All present enzymatic activity of Replacement of Catalytic Histidine-195 of Chloramphenicol Acetyltransferase: Evidence For A General Base Role For Glutamate:
2.3.1.28;

The structure of Replacement of Catalytic Histidine-195 of Chloramphenicol Acetyltransferase: Evidence For A General Base Role For Glutamate, PDB code: 1cia was solved by A.G.W.Leslie, M.R.Gibbs, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	6.00 / 2.50
Space group	H 3 2
Cell size a, b, c (Å), α, β, γ (°)	107.640, 107.640, 124.130, 90.00, 90.00, 120.00
R / Rfree (%)	n/a / n/a

The binding sites of Cobalt atom in the Replacement of Catalytic Histidine-195 of Chloramphenicol Acetyltransferase: Evidence For A General Base Role For Glutamate (pdb code 1cia). This binding sites where shown within 5.0 Angstroms radius around Cobalt atom.
In total 2 binding sites of Cobalt where determined in the Replacement of Catalytic Histidine-195 of Chloramphenicol Acetyltransferase: Evidence For A General Base Role For Glutamate, PDB code: 1cia:
Jump to Cobalt binding site number: 1; 2;

Cobalt binding site 1 out of 2 in the Replacement of Catalytic Histidine-195 of Chloramphenicol Acetyltransferase: Evidence For A General Base Role For Glutamate


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 1 of Replacement of Catalytic Histidine-195 of Chloramphenicol Acetyltransferase: Evidence For A General Base Role For Glutamate within 5.0Å range: probe atom residue distance (Å) B Occ A:Co222 b:11.4 occ:0.50 OE2 A:GLU23 1.9 18.0 1.0 ND1 A:HIS27 2.0 19.7 1.0 CD A:GLU23 2.6 12.2 1.0 OE1 A:GLU23 2.7 14.5 1.0 CE1 A:HIS27 2.9 17.9 1.0 CG A:HIS27 3.0 17.5 1.0 CB A:HIS27 3.5 15.7 1.0 NE2 A:HIS27 4.0 18.6 1.0 CG A:GLU23 4.1 9.6 1.0 O A:HOH267 4.1 38.9 1.0 CD2 A:HIS27 4.1 17.5 1.0 O A:HOH256 4.4 24.7 1.0 O A:HOH268 4.4 38.2 1.0 CA A:HIS27 5.0 15.6 1.0

Cobalt binding site 2 out of 2 in the Replacement of Catalytic Histidine-195 of Chloramphenicol Acetyltransferase: Evidence For A General Base Role For Glutamate


Mono view


Stereo pair view

A full contact list of Cobalt with other atoms in the Co binding site number 2 of Replacement of Catalytic Histidine-195 of Chloramphenicol Acetyltransferase: Evidence For A General Base Role For Glutamate within 5.0Å range: probe atom residue distance (Å) B Occ A:Co223 b:61.7 occ:0.50 O A:HOH341 1.4 69.5 0.5 O A:ASN68 4.0 18.3 1.0 O A:ASP87 4.3 23.9 1.0

A.Lewendon, I.A.Murray, W.V.Shaw, M.R.Gibbs, A.G.Leslie. Replacement of Catalytic Histidine-195 of Chloramphenicol Acetyltransferase: Evidence For A General Base Role For Glutamate. Biochemistry V. 33 1944 1994.
ISSN: ISSN 0006-2960
PubMed: 7906544
DOI: 10.1021/BI00173A043